Search Results - "Stedtfeld, Tiffany M."

In-feed antibiotic effects on the swine intestinal microbiome by Looft, Torey, Johnson, Timothy A., Allen, Heather K., Bayles, Darrell O., Alt, David P., Stedtfeld, Robert D., Sul, Woo Jun, Stedtfeld, Tiffany M., Chai, Benli, Cole, James R., Hashsham, Syed A., Tiedje, James M., Stanton, Thad B.

“…Antibiotics have been administered to agricultural animals for disease treatment disease prevention, and growth promotion for over 50 y. The impact of such…”
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Primer set 2.0 for highly parallel qPCR array targeting antibiotic resistance genes and mobile genetic elements by Stedtfeld, Robert D, Guo, Xueping, Stedtfeld, Tiffany M, Sheng, Hongjie, Williams, Maggie R, Hauschild, Kristin, Gunturu, Santosh, Tift, Leo, Wang, Fang, Howe, Adina, Chai, Benli, Yin, Daqiang, Cole, James R, Tiedje, James M, Hashsham, Syed A

“…The high-throughput antibiotic resistance gene (ARG) qPCR array, initially published in 2012, is increasingly used to quantify resistance and mobile…”
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Antimicrobial resistance dashboard application for mapping environmental occurrence and resistant pathogens by Stedtfeld, Robert D., Williams, Maggie R., Fakher, Umama, Johnson, Timothy A., Stedtfeld, Tiffany M., Wang, Fang, Khalife, Walid T., Hughes, Mary, Etchebarne, Brett E., Tiedje, James M., Hashsham, Syed A.

“…An antibiotic resistance (AR) Dashboard application is being developed regarding the occurrence of antibiotic resistance genes (ARG) and bacteria (ARB) in…”
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TCDD administered on activated carbon eliminates bioavailability and subsequent shifts to a key murine gut commensal by Stedtfeld, Robert D., Brett Sallach, J., Crawford, Robert B., Stedtfeld, Tiffany M., Williams, Maggie R., Waseem, Hassan, Johnston, Cliff T., Li, Hui, Teppen, Brian J., Kaminski, Norbert E., Boyd, Stephen A., Tiedje, James M., Hashsham, Syed A.

“…Activated carbon (AC) is an increasingly attractive remediation alternative for the sequestration of dioxins at contaminated sites globally. However, the…”
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Evaluation of Nucleic Acid Isothermal Amplification Methods for Human Clinical Microbial Infection Detection by Etchebarne, Brett E, Li, Zenggang, Stedtfeld, Robert D, Nicholas, Michael C, Williams, Maggie R, Johnson, Timothy A, Stedtfeld, Tiffany M, Kostic, Tanja, Khalife, Walid T, Tiedje, James M, Hashsham, Syed A, Hughes, Mary J

“…Battling infection is a major healthcare objective. Untreated infections can rapidly evolve toward the condition of sepsis in which the body begins to fail and…”
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Development and Experimental Validation of a Predictive Threshold Cycle Equation for Quantification of Virulence and Marker Genes by High-Throughput Nanoliter-Volume PCR on the OpenArray Platform by Stedtfeld, Robert D, Baushke, Samuel W, Tourlousse, Dieter M, Miller, Sarah M, Stedtfeld, Tiffany M, Gulari, Erdogan, Tiedje, James M, Hashsham, Syed A

“…Development of quantitative PCR (QPCR) assays typically requires extensive screening within and across a given species to ensure specific detection and lucid…”
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Modulatory Influence of Segmented Filamentous Bacteria on Transcriptomic Response of Gnotobiotic Mice Exposed to TCDD by Stedtfeld, Robert D, Chai, Benli, Crawford, Robert B, Stedtfeld, Tiffany M, Williams, Maggie R, Xiangwen, Shao, Kuwahara, Tomomi, Cole, James R, Kaminski, Norbert E, Tiedje, James M, Hashsham, Syed A

“…Environmental toxicants such as 2,3,7,8-tetrachlorodibenzo- -dioxin (TCDD), an aryl hydrocarbon receptor (AhR), are known to induce host toxicity and…”
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Influence of Soil Characteristics and Proximity to Antarctic Research Stations on Abundance of Antibiotic Resistance Genes in Soils by Wang, Fang, Stedtfeld, Robert D, Kim, Ok-Sun, Chai, Benli, Yang, Luxi, Stedtfeld, Tiffany M, Hong, Soon Gyu, Kim, Dockyu, Lim, Hyoun Soo, Hashsham, Syed A, Tiedje, James M, Sul, Woo Jun

“…Soil is an important environmental reservoir of antibiotic resistance genes (ARGs), which are increasingly recognized as environmental contaminants. Methods to…”
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Gene-Z: a device for point of care genetic testing using a smartphone by Stedtfeld, Robert D, Tourlousse, Dieter M, Seyrig, Gregoire, Stedtfeld, Tiffany M, Kronlein, Maggie, Price, Scott, Ahmad, Farhan, Gulari, Erdogan, Tiedje, James M, Hashsham, Syed A

“…By 2012, point of care (POC) testing will constitute roughly one third of the $59 billion in vitro diagnostics market. The ability to carry out multiplexed…”
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MicroRNA-based host response to toxicant exposure is influenced by the presence of gut microbial populations by Williams, Maggie R., Stedtfeld, Robert D., Stedtfeld, Tiffany M., Crawford, Robert B., Kuwahara, Tomomi, Kaminski, Norbert E., Tiedje, James M., Hashsham, Syed A.

“…Segmented filamentous bacteria (SFB) and Bacteroides fragilis are known to interact with the host immune response through the aryl hydrocarbon receptor (Ahr)…”
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Alterations in the Endophyte-Enriched Root-Associated Microbiome of Rice Receiving Growth-Promoting Treatments of Urea Fertilizer and Rhizobium Biofertilizer by Jha, Prabhat N., Gomaa, Abu-Bakr, Yanni, Youssef G., El-Saadany, Abd-Elgawad Y., Stedtfeld, Tiffany M., Stedtfeld, Robert D., Gantner, Stephan, Chai, Benli, Cole, James, Hashsham, Syed A., Dazzo, Frank B.

“…We examined the bacterial endophyte–enriched root-associated microbiome within rice (Oryza sativa) 55 days after growth in soil with and without urea…”
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On-filter direct amplification of Legionella pneumophila for rapid assessment of its abundance and viability by Samhan, Farag A., Stedtfeld, Tiffany M., Waseem, Hassan, Williams, Maggie R., Stedtfeld, Robert D., Hashsham, Syed A.

“…Guidelines and regulations to control Legionella pneumophila in cooling water systems of large buildings are evolving due to the increasing number of…”
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Thirty-minute screening of antibiotic resistance genes in bacterial isolates with minimal sample preparation in static self-dispensing 64 and 384 assay cards by Kostić, Tanja, Ellis, Michael, Williams, Maggie R, Stedtfeld, Tiffany M, Kaneene, John B, Stedtfeld, Robert D, Hashsham, Syed A

“…In a clinical setting, molecular assays such as polymerase chain reaction offer a rapid means to infer or confirm identity and therapeutic decisions…”
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TCDD influences reservoir of antibiotic resistance genes in murine gut microbiome by Stedtfeld, Robert D., Stedtfeld, Tiffany M., Fader, Kelly A., Williams, Maggie R., Bhaduri, Prianca, Quensen, John, Zacharewski, Timothy R., Tiedje, James M., Hashsham, Syed A.

“…Abstract Dysbiosis of the gut microbiome via antibiotics, changes in diet and infection can select for bacterial groups that more frequently harbor…”
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Isothermal assay targeting class 1 integrase gene for environmental surveillance of antibiotic resistance markers by Stedtfeld, Robert D., Stedtfeld, Tiffany M., Waseem, Hassen, Fitschen-Brown, Meridith, Guo, Xueping, Chai, Benli, Williams, Maggie R., Shook, Trevor, Logan, Amanda, Graham, Ally, Chae, Jong-Chan, Sul, Woo-Jun, VanHouten, Jacob, Cole, James R., Zylstra, Gerben J., Tiedje, James M., Upham, Brad L., Hashsham, Syed A.

“…Antimicrobial resistance genes (ARGs) present in the environment pose a risk to human health due to potential for transfer to human pathogens. Surveillance is…”
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DNA Extraction-Free Quantification of Dehalococcoides spp. in Groundwater Using a Hand-Held Device by Stedtfeld, Robert D, Stedtfeld, Tiffany M, Kronlein, Maggie, Seyrig, Gregoire, Steffan, Robert J, Cupples, Alison M, Hashsham, Syed A

“…Nucleic acid amplification of biomarkers is increasingly used to measure microbial activity and predict remedial performance in sites with trichloroethene…”
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Direct loop mediated isothermal amplification on filters for quantification of Dehalobacter in groundwater by Stedtfeld, Robert D, Stedtfeld, Tiffany M, Samhan, Farag, Kanitkar, Yogendra H, Hatzinger, Paul B., Cupples, Alison M, Hashsham, Syed A

“…Nucleic acid amplification of biomarkers is increasingly used to monitor microbial activity and assess remedial performance in contaminated aquifers. Previous…”
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Quantification of microRNAs directly from body fluids using a base-stacking isothermal amplification method in a point-of-care device by Williams, Maggie R., Stedtfeld, Robert D., Stedtfeld, Tiffany M., Tiedje, James M., Hashsham, Syed A.

“…MicroRNAs have been proposed to be a class of biomarkers of disease as expression levels are significantly altered in various tissues and body fluids when…”
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Static self-directed sample dispensing into a series of reaction wells on a microfluidic card for parallel genetic detection of microbial pathogens by Stedtfeld, Robert D., Liu, Yen-Cheng, Stedtfeld, Tiffany M., Kostic, Tanja, Kronlein, Maggie, Srivannavit, Onnop, Khalife, Walid T., Tiedje, James M., Gulari, Erdogan, Hughes, Mary, Etchebarne, Brett, Hashsham, Syed A.

“…A microfluidic card is described for simultaneous and rapid genetic detection of multiple microbial pathogens. The hydrophobic surface of native acrylic and a…”
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Alterations in the Endophyte-Enriched Root-Associated Microbiome of Rice Receiving Growth-Promoting Treatments of Urea Fertilizer and Rhizobium Biofertilizer by Jha, Prabhat N., Gomaa, Abu-Bakr, Yanni, Youssef G., El-Saadany, Abd-Elgawad Y., Stedtfeld, Tiffany M., Stedtfeld, Robert D., Gantner, Stephan, Chai, Benli, Cole, James, Hashsham, Syed A., Dazzo, Frank B.

“…Not provided…”
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