Search Results - "Staros, J V"

Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions by Staros, J V, Wright, R W, Swingle, D M

“…Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However,…”
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Real-Time Kinetics of Ligand/Cell Surface Receptor Interactions in Living Cells:  Binding of Epidermal Growth Factor to the Epidermal Growth Factor Receptor by Wilkinson, John C, Stein, Richard A, Guyer, Cheryl A, Beechem, Joseph M, Staros, James V

“…We describe a system for extending stopped-flow analysis to the kinetics of ligand capture and release by cell surface receptors in living cells. While most…”
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A Kinase-Negative Epidermal Growth Factor Receptor that Retains the Capacity to Stimulate DNA Synthesis by Coker, Kenneth J., Staros, James V., Guyer, Cheryl A.

“…The residue proposed to serve as the catalytic base for phosphoryl transfer, Asp-813, of the human epidermal growth factor receptor (EGFR) was mutated to Ala,…”
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Affinity labeling of the protein kinase associated with the epidermal growth factor receptor in membrane vesicles from A431 cells by Buhrow, S A, Cohen, S, Staros, J V

“…Epidermal growth factor (EGF), a mitogenic polypeptide hormone, stimulates the phosphorylation of certain endogenous proteins in membrane preparations derived…”
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Direct Identification of Residues of the Epidermal Growth Factor Receptor in Close Proximity to the Amino Terminus of Bound Epidermal Growth Factor by Woltjer, Randall L., Lukas, Thomas J., Staros, James V.

“…We have recently developed a kinetically controlled, step-wise affinity cross-linking technique for specific, high-yield, covalent linkage of murine epidermal…”
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High-yield trapping of EGF-induced receptor dimers by chemical cross-linking by Fanger, B O, Stephens, J E, Staros, J V

“…The binding of epidermal growth factor (EGF) to its plasma membrane receptor results in the stimulation of a tyrosyl residue-specific protein kinase, which has…”
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Identification of residues in the nucleotide binding site of the epidermal growth factor receptor/kinase by Russo, M W, Lukas, T J, Cohen, S, Staros, J V

“…We have purified the epidermal growth factor receptor/kinase from A431 membrane vesicles which had been affinity labeled with the ATP analog,…”
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Binding specificity of the periplasmic oligopeptide-binding protein from Escherichia coli by GUYER, C. A, MORGAN, D. G, STAROS, J. V

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Evolutionary analysis of the ErbB receptor and ligand families by Stein, R A, Staros, J V

“…We have compared all available deduced protein sequences of the ErbB family of receptors and their ligands. Analysis of the aligned sequences of the receptors…”
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Characterization of the interaction of 5'-p-fluorosulfonylbenzoyl adenosine with the epidermal growth factor receptor/protein kinase in A431 cell membranes by Buhrow, S A, Cohen, S, Garbers, D L, Staros, J V

“…Treatment of membrane vesicles from A431 cells, a human epidermoid carcinoma line, with the affinity label 5'-p-fluorosulfonylbenzoyl [8-14C]adenosine…”
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Ligand- and kinase activity-independent cell survival mediated by the epidermal growth factor receptor expressed in 32D cells by Ewald, Jonathan A., Wilkinson, John C., Guyer, Cheryl A., Staros, James V.

“…To investigate the intrinsic activities of the epidermal growth factor receptor and the role of its kinase domain in these functions within a cellular…”
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Purification and characterization of a periplasmic oligopeptide binding protein from Escherichia coli by Guyer, C A, Morgan, D G, Osheroff, N, Staros, J V

“…We have purified and characterized an oligopeptide binding protein released from the periplasm of Escherichia coli W by mild osmotic shock. The purified…”
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Effect of ErbB2 Coexpression on the Kinetic Interactions of Epidermal Growth Factor with Its Receptor in Intact Cells by Wilkinson, John C, Staros, James V

“…We have extended the use of stopped-flow mixing and fluorescence anisotropy detection to investigate in real-time the effects of ErbB2 coexpression on the…”
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High-yield covalent attachment of epidermal growth factor to its receptor by kinetically controlled, stepwise affinity cross-linking by Woltjer, Randall L, Weclas-Henderson, Ludmila, Papayannopoulos, Ioannis A, Staros, James V

“…We report here the use of a stepwise affinity cross-linking technique in the specific covalent attachment of epidermal growth factor (EGF) to its receptor. A…”
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Preparation and characterization of a bifunctionally spin-labeled mutant of murine epidermal growth factor for saturation-transfer electron paramagnetic resonance studies of the growth factor/receptor complex by Rousseau, Dennis L, Guyer, Cheryl A, Beth, Albert H, Papayannopoulos, Ioannis A, Wang, Baiyang, Wu, Ray, Mroczkowski, Barbara, Staros, James V

“…In this report we describe the production of a [Lys3,Tyr22] murine epidermal growth factor (mEGF) mutant for spin-labeling with…”
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An Analytical Approach to the Measurement of Equilibrium Binding Constants:  Application to EGF Binding to EGF Receptors in Intact Cells Measured by Flow Cytometry by Stein, Richard A, Wilkinson, John C, Guyer, Cheryl A, Staros, James V

“…In ligand binding studies, ligand depletion often limits the accuracy of the results obtained. This problem is approached by employing the simple observation…”
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Demonstration by covalent cross-linking of a specific interaction between beta-endorphin and calmodulin by Giedroc, D P, Puett, D, Ling, N, Staros, J V

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Reactions of N-hydroxysulfosuccinimide active esters by Anjaneyulu, P S, Staros, J V

“…N-Hydroxysulfosuccinimide esters are reactive functional groups employed in a variety of protein modification reagents, especially cross-linking reagents. For…”
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Effects of diethyl ether on membrane lipid ordering and on rotational dynamics of the anion exchange protein in intact human erythrocytes: correlations with anion exchange function by Cobb, Charles E, Juliao, Saul F, Balasubramanian, K, Staros, James V, Beth, Albert H

“…The roles of lipid ordering and protein dynamics on the function of the anion exchange protein (band 3) in intact human erythrocytes have been investigated…”
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Dynamics and interactions of the anion channel in intact human erythrocytes: an electron paramagnetic resonance spectroscopic study employing a new membrane-impermeant bifunctional spin-label by Beth, Albert H, Conturo, Thomas E, Venkataramu, S. D, Staros, James V

“…We have developed a new membrane-impermeant, bifunctional spin-labeling reagent, bis-(sulfo-N-succinimidyl) doxyl-2-spiro-4'-pimelate (BSSDP), and employed it…”
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