Search Results - "Stapleton, James"
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Development of an in vitro compartmentalization screen for high-throughput directed evolution of [FeFe] hydrogenases
Published in PloS one (06-12-2010)“…[FeFe] hydrogenase enzymes catalyze the formation and dissociation of molecular hydrogen with the help of a complex prosthetic group composed of common…”
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Changes of Fusarium oxysporum f.sp. lactucae levels and soil microbial community during soil biosolarization using chitin as soil amendment
Published in PloS one (05-05-2020)“…Regulatory pressure along with environmental and human health concerns drive the development of soil fumigation alternatives such as soil biosolarization…”
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3
High-resolution sequence-function mapping of full-length proteins
Published in PloS one (19-03-2015)“…Comprehensive sequence-function mapping involves detailing the fitness contribution of every possible single mutation to a gene by comparing the abundance of…”
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4
A cell-free microtiter plate screen for improved [FeFe] hydrogenases
Published in PloS one (10-05-2010)“…[FeFe] hydrogenase enzymes catalyze the production and dissociation of H(2), a potential renewable fuel. Attempts to exploit these catalysts in engineered…”
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5
Computational redesign of the lipid-facing surface of the outer membrane protein OmpA
Published in Proceedings of the National Academy of Sciences - PNAS (04-08-2015)“…Advances in computational design methods have made possible extensive engineering of soluble proteins, but designed β-barrel membrane proteins await…”
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6
Haplotype-Phased Synthetic Long Reads from Short-Read Sequencing
Published in PloS one (20-01-2016)“…Next-generation DNA sequencing has revolutionized the study of biology. However, the short read lengths of the dominant instruments complicate assembly of…”
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Assessment of tomato and wine processing solid wastes as soil amendments for biosolarization
Published in Waste management (Elmsford) (01-02-2016)“…[Display omitted] •The biosolarization potential of tomato and grape processing wastes were assessed.•Simulated biosolarization gauged pest inactivation…”
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Almond processing residues as a source of organic acid biopesticides during biosolarization
Published in Waste management (Elmsford) (01-01-2020)“…•Feasibility of almond by-products was demonstrated for use in biosolarization.•Treated soils experienced pH depression and organic acid accumulation.•Residue…”
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Assessment of Two Solid Anaerobic Digestate Soil Amendments for Effects on Soil Quality and Biosolarization Efficacy
Published in Journal of agricultural and food chemistry (03-05-2017)“…Anaerobic digestion is an organic waste bioconversion process that produces biofuel and digestates. Digestates have potential to be applied as soil amendment…”
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10
Tyrosine, cysteine, and S-adenosyl methionine stimulate in vitro [FeFe] hydrogenase activation
Published in PloS one (26-10-2009)“…[FeFe] hydrogenases are metalloenzymes involved in the anaerobic metabolism of H(2). These proteins are distinguished by an active site cofactor known as the…”
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11
Quantitative and simultaneous translational control of distinct mammalian mRNAs
Published in Nucleic acids research (01-07-2013)“…The introduction of multiple genes into cells is increasingly required for understanding and engineering biological systems. Small-molecule-responsive…”
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Weed seed inactivation in soil mesocosms via biosolarization with mature compost and tomato processing waste amendments
Published in Pest management science (01-05-2017)“…BACKGROUND Biosolarization is a fumigation alternative that combines passive solar heating with amendment‐driven soil microbial activity to temporarily create…”
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Characterization of bacterial communities in solarized soil amended with lignocellulosic organic matter
Published in Applied soil ecology : a section of Agriculture, ecosystems & environment (01-01-2014)“…•Microbial community structures in solarized soils are studied.•Soils were amended with mature compost and organic matter prior to solarization.•Bacterial…”
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The effect of circular soil biosolarization treatment on the physiology, metabolomics, and microbiome of tomato plants under certain abiotic stresses
Published in Frontiers in plant science (08-11-2022)“…Soil biosolarization (SBS) is an alternative technique for soil pest control to standard techniques such as soil fumigation and soil solarization (SS). By…”
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Effects of Short-Term Biosolarization Using Mature Compost and Industrial Tomato Waste Amendments on the Generation and Persistence of Biocidal Soil Conditions and Subsequent Tomato Growth
Published in Journal of agricultural and food chemistry (06-06-2018)“…Conventional solarization and biosolarization with mature compost and tomato processing residue amendments were compared with respect to generation of…”
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Managing compost stability and amendment to soil to enhance soil heating during soil solarization
Published in Waste management (Elmsford) (01-05-2013)“…► We examine heat generation during solarization of soil amended with organic matter. ► Biological activity and phytotoxicity in solarized amended soil were…”
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Fine Epitope Mapping of Two Antibodies Neutralizing the Bordetella Adenylate Cyclase Toxin
Published in Biochemistry (Easton) (07-03-2017)“…Adenylate cyclase toxin (ACT) is an important Bordetella pertussis virulence factor that is not included in current acellular pertussis vaccines. We previously…”
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Effect of management of organic wastes on inactivation of Brassica nigra and Fusarium oxysporum f.sp. lactucae using soil biosolarization
Published in Pest management science (01-08-2018)“…BACKGROUND Soil biosolarization is a promising alternative to conventional fumigation. Volatile fatty acids (VFAs) produced in the soil through fermentation of…”
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Plasmid-based one-pot saturation mutagenesis
Published in Nature methods (01-11-2016)“…Nicking mutagenesis is a streamlined protocol for plasmid-based single-pot comprehensive saturation mutagenesis. Deep mutational scanning is a foundational…”
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A life cycle assessment of biosolarization as a valorization pathway for tomato pomace utilization in California
Published in Journal of cleaner production (10-01-2017)“…The California tomato processing industry produced circa 388,856 t of tomato pomace in 2014. While currently used for animal feed, tomato pomace could be…”
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