Carbapenemase Production of Clinical Isolates Acinetobacter baumannii and Pseudomonas aeruginosa from a Bulgarian University Hospital

Production of Bla OXA-23, OXA-24, OXA-58 and hyperexpression of OXA-51 due to ISAba1 insertion sequence are the leading causes of carbapenem resistance in Acinetobacter baumannii. The loss of OprD transmembrane protein and the overexpression of some effl ux pumps are considered to be the main factor...

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Published in:	Folia Medica Vol. 59; no. 4; pp. 413 - 422
Main Authors:	Petrova, Atanaska P, Stanimirova, Irina D, Ivanov, Ivan N, Petrov, Michael M, Miteva-Katrandzhieva, Tsonka M, Grivnev, Vasil I, Kardjeva, Velichka S, Kantardzhiev, Todor V, Murdjeva, Mariana A
Format:	Journal Article
Language:	English
Published:	Bulgaria Pensoft Publishers 20-12-2017
Subjects:	Acinetobacter baumannii - drug effects Acinetobacter baumannii - enzymology Bacterial Proteins - biosynthesis beta-Lactamases - biosynthesis Bulgaria carbapenemases Carbapenems - pharmacology clonality Drug Resistance, Bacterial genetic methods Gram negative non-fermenters Hospitals, University Humans Microbial Sensitivity Tests phenotypic tests Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - enzymology Bulgaria phenotypic tests clonality carbapenemases Gram negative non-fermenters genetic methods
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Summary:	Production of Bla OXA-23, OXA-24, OXA-58 and hyperexpression of OXA-51 due to ISAba1 insertion sequence are the leading causes of carbapenem resistance in Acinetobacter baumannii. The loss of OprD transmembrane protein and the overexpression of some effl ux pumps are considered to be the main factors for carbapenem resistance in Pseudomonas aeruginosa whereas metallo-enzymes' production has a secondary role. Тo examine the carbapenem resistance due to carbapenemase production among clinically signifi cant Gram-negative non-fermenters from St George University hospital, Plovdiv: A. baumannii and P. aeruginosa. Forty three A. baumannii and 43 P. aeruginosa isolates, resistant or with intermediate resistance to imipenem and/or meropenem were included in the study. They were collected from patients admitted in 14 various hospital wards between 2010 and 2014. Both phenotypic and genetic methods were used for identifi cation and antimicrobial susceptibility testing. All A. baumannii demonstrated carbapenemase production determined by a modifi ed Hodge test whereas P. aeruginosa isolates did not show this phenomenon. OXA-23 genes were determined in 97.7% (42 out of 43) of A. baumannii isolates indistinguishable from the sequence of the classical ARI-1 gene. OXA-24, OXA-58 and overexpression of OXA-51 were not registered in any of the isolates. All P. aeruginosa were negative for blaVIM and blaIMP genes. The leading cause of carbapenem resistance in A. baumannii isolates from our hospital is the carbapenemase production due to the expression of OXA- 23 gene, whereas in P. aeruginosa - the loss of transmembrane OprD protein and the effl ux pumps' hyperexpression are suspected to be the main mechanisms.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0204-8043 1314-2143
DOI:	10.1515/folmed-2017-0060