Search Results - "Stahley, Mary R."

Optimization of mRNA untranslated regions for improved expression of therapeutic mRNA by Asrani, Kirtika H., Farelli, Jeremiah D., Stahley, Mary R., Miller, Rebecca L., Cheng, Christopher J., Subramanian, Romesh R., Brown, Jeffrey M.

“…mRNA based therapies hold great promise for the treatment of genetic diseases. However, this therapeutic approach suffers from multiple challenges including…”
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Structural Evidence for a Two-Metal-Ion Mechanism of Group I Intron Splicing by Stahley, Mary R, Strobel, Scott A

“…We report the 3.4 angstrom crystal structure of a catalytically active group I intron splicing intermediate containing the complete intron, both exons, the…”
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A Transient Kinetic Analysis of PRMT1 Catalysis by Feng, You, Xie, Nan, Jin, Miyeong, Stahley, Mary R, Stivers, James T, Zheng, Yujun George

“…Post-translational modifications (PTMs) are important strategies used by eukaryotic organisms to modulate their phenotypes. One of the well-studied PTMs,…”
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RNA splicing: group I intron crystal structures reveal the basis of splice site selection and metal ion catalysis by Stahley, Mary R, Strobel, Scott A

“…The group I intron has served as a model for RNA catalysis since its discovery 25 years ago. Four recently determined high-resolution crystal structures…”
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High Apparent Dielectric Constant Inside a Protein Reflects Structural Reorganization Coupled to the Ionization of an Internal Asp by Karp, Daniel A., Gittis, Apostolos G., Stahley, Mary R., Fitch, Carolyn A., Stites, Wesley E., García-Moreno E., Bertrand

“…The dielectric properties of proteins are poorly understood and difficult to describe quantitatively. This limits the accuracy of methods for structure-based…”
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Conformational Consequences of Ionization of Lys, Asp, and Glu Buried at Position 66 in Staphylococcal Nuclease by Karp, Daniel A, Stahley, Mary R, García-Moreno E, Bertrand

“…The pK a values measured previously for the internal Lys-66, Asp-66, and Glu-66 in variants of a highly stable form of staphylococcal nuclease are shifted by…”
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In vivo CRISPR base editing of PCSK9 durably lowers cholesterol in primates by Musunuru, Kiran, Chadwick, Alexandra C., Mizoguchi, Taiji, Garcia, Sara P., DeNizio, Jamie E., Reiss, Caroline W., Wang, Kui, Iyer, Sowmya, Dutta, Chaitali, Clendaniel, Victoria, Amaonye, Michael, Beach, Aaron, Berth, Kathleen, Biswas, Souvik, Braun, Maurine C., Chen, Huei-Mei, Colace, Thomas V., Ganey, John D., Gangopadhyay, Soumyashree A., Garrity, Ryan, Kasiewicz, Lisa N., Lavoie, Jennifer, Madsen, James A., Matsumoto, Yuri, Mazzola, Anne Marie, Nasrullah, Yusuf S., Nneji, Joseph, Ren, Huilan, Sanjeev, Athul, Shay, Madeleine, Stahley, Mary R., Fan, Steven H. Y., Tam, Ying K., Gaudelli, Nicole M., Ciaramella, Giuseppe, Stolz, Leslie E., Malyala, Padma, Cheng, Christopher J., Rajeev, Kallanthottathil G., Rohde, Ellen, Bellinger, Andrew M., Kathiresan, Sekar

“…Gene-editing technologies, which include the CRISPR–Cas nucleases 1 – 3 and CRISPR base editors 4 , 5 , have the potential to permanently modify…”
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Crystal structure of a group I intron splicing intermediate by Adams, Peter L, Stahley, Mary R, Gill, Michelle L, Kosek, Anne B, Wang, Jimin, Strobel, Scott A

“…A recently reported crystal structure of an intact bacterial group I self-splicing intron in complex with both its exons provided the first molecular view into…”
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Crystal structure of a self-splicing group I intron with both exons by Wang, Jimin, Strobel, Scott A, Adams, Peter L, Stahley, Mary R, Kosek, Anne B

“…The discovery of the RNA self-splicing group I intron provided the first demonstration that not all enzymes are proteins. Here we report the X-ray crystal…”
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Enzymatic Excision of Uracil Residues in Nucleosomes Depends on the Local DNA Structure and Dynamics by Ye, Yu, Stahley, Mary R, Xu, Jianqing, Friedman, Joshua I, Sun, Yan, McKnight, Jeffrey N, Gray, Jeffrey J, Bowman, Gregory D, Stivers, James T

“…The excision of uracil bases from DNA is accomplished by the enzyme uracil DNA glycosylase (UNG). Recognition of uracil bases in free DNA is facilitated by…”
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Leveraging Rational Protein Engineering to Improve mRNA Therapeutics by Farelli, Jeremiah D, Asrani, Kirtika H, Isaacs, Cleo, deBear, Joanna S, Stahley, Mary R, Shah, Anumeha, Lasaro, Melissa A, Cheng, Christopher J, Subramanian, Romesh R

“…Messenger RNA (mRNA) is a promising new class of therapeutics that has potential for treatment of diseases in fields such as immunology, oncology, vaccines,…”
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Mechanism and Specificity of DNA Strand Exchange Catalyzed by Vaccinia DNA Topoisomerase Type I by Stahley, Mary R, Stivers, James T

“…The type I DNA topoisomerase from vaccinia virus (vTopo) forms a reversible covalent 3′-phosphotyrosyl linkage with a single strand of duplex DNA at the…”
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Structural Metals in the Group I Intron: A Ribozyme with a Multiple Metal Ion Core by Stahley, Mary R., Adams, Peter L., Wang, Jimin, Strobel, Scott A.

“…Metal ions play key roles in the folding and function for many structured RNAs, including group I introns. We determined the X-ray crystal structure of the…”
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RNA kink turns to the left and to the right by Strobel, Scott A, Adams, Peter L, Stahley, Mary R, Wang, Jimin

“…A helix-loop-helix within the group I intron has most of the canonical sequence elements of a kink turn (K-turn), yet it bends in the opposite direction. The…”
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RNA kink turns to the left and to the right: FIGURE 1 by STROBEL, SCOTT A., ADAMS, PETER L., STAHLEY, MARY R., WANG, JIMIN

“…A helix-loop-helix within the group I intron has most of the canonical sequence elements of a kink turn (K-turn), yet it bends in the opposite direction. The…”
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