Full-genome analysis and pathogenicity of a genetically distinct Russian PRRSV-1 Tyu16 strain

•The full genome sequence of the Russian group subtype 1 PRRSV-1 Tyu16 strain is determined.•The Tyu16 strain genome demonstrated a varying nucleotide identity to the reference PRRSV-1.•Demonstrated low pathogenicity of the Russian group subtype 1 PRRSV-1 Tyu16 strain. Porcine reproductive and respi...

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Published in:	Veterinary microbiology Vol. 247; p. 108784
Main Authors:	Yuzhakov, Anton G., Raev, Sergei A., Shchetinin, Alexey M., Gushchin, Vladimir A., Alekseev, Konstantin P., Stafford, Viсtoria V., Komina, Alina K., Zaberezhny, Alexei D., Gulyukin, Alexey M., Aliper, Taras I.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-08-2020 Elsevier BV
Subjects:	Animal diseases Animals Antibodies, Viral - blood Cells, Cultured Dyspnea Experimental infection Fever Genome Genome, Viral Genomes Genotype Macrophages, Alveolar - virology Nucleotide sequence Open Reading Frames Pathogenicity Phylogeny Porcine Reproductive and Respiratory Syndrome - virology Porcine respiratory and reproductive syndrome virus - classification Porcine respiratory and reproductive syndrome virus - genetics Porcine respiratory and reproductive syndrome virus - pathogenicity PRRSV Recombination Russia Sequence Analysis, DNA Strains (organisms) Subtype Swine Swine Diseases - virology Viremia Viremia - veterinary Virulence Virulence - genetics Virus Shedding Whole Genome Sequencing Russia aa ORF RNA nt PRRS Virulence Genotype nsp PCV2 TCID50 Pathogenicity RT-PCR CPE IPMA cDNA dpi Genome PAM Subtype PCR PRRSV ELISA
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Summary:	•The full genome sequence of the Russian group subtype 1 PRRSV-1 Tyu16 strain is determined.•The Tyu16 strain genome demonstrated a varying nucleotide identity to the reference PRRSV-1.•Demonstrated low pathogenicity of the Russian group subtype 1 PRRSV-1 Tyu16 strain. Porcine reproductive and respiratory syndrome virus-1 (PRRSV-1) strains from Eastern Europe have a high diversity. All three known subtypes (1, 2, 3) of PRRSV-1 have been detected in Russia. There are two different groups of viruses belonging to the subtype 1: pan-European subtype 1 strains, and insufficiently studied Russian strains. The main objective of this study was to characterize the full genomic structure of the atypical Tyu16 strain of the Russian group subtype 1 PRRSV-1 and to assess its pathogenicity. Complete sequencing of the Tyu16 strain revealed that it did not belong to any existing subtype. Comparison of the whole genome sequence of the Tyu16 strain with that of PRRSV-1 prototype strains revealed 78.1 % (subtype 1 Lelystad), 78.1 % (subtype 2 WestSib13) and 77.7 % (subtype 3 Lena) nucleotide identity level, respectively. The coding sequence of different parts of the Tyu16 strain genome demonstrated a varying percentage identity to the different reference PRRSV-1 strains, which may indicate recombination events in its evolutionary history. We assume that among PRRSV-1 isolates, the Tyu16 is the closest relative to the common ancestor of PRRSV-1 and PRRSV-2. Low pathogenicity of the Tyu16 was demonstrated by experimental infection of 70-day-old piglets. Infected animals showed fever not exceeding 7 days, dyspnea in two out of five pigs and reduced weight gain. The virus shedding was undetectable and viremia was at low level.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-1135 1873-2542
DOI:	10.1016/j.vetmic.2020.108784