Search Results - "Stacey, G N"

Guidelines for the use of cell lines in biomedical research by GERAGHTY, R. J, CAPES-DAVIS, A, THRAVES, P, VIAS, M, DAVIS, J. M, DOWNWARD, J, FRESHNEY, R. I, KNEZEVIC, I, LOVELL-BADGE, R, MASTERS, J. R. W, MEREDITH, J, STACEY, G. N

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Guidelines for the use of cell lines in biomedical research by Geraghty, R J, Capes-Davis, A, Davis, J M, Downward, J, Freshney, R I, Knezevic, I, Lovell-Badge, R, Masters, J R W, Meredith, J, Stacey, G N, Thraves, P, Vias, M

“…Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic and phenotypic, are among the…”
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Cell contamination leads to inaccurate data: we must take action now by Stacey, G. N

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Assessment of mumps virus growth on various continuous cell lines by virological, immunological, molecular and morphological investigations by Afzal, M.A., Dussupt, V., Minor, P.D., Pipkin, P.A., Fleck, R., Hockley, D.J., Stacey, G.N.

“…Vero cells have been used as a convenient laboratory substrate for the isolation of mumps virus but may not be very sensitive and may select for particular…”
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Standardisation of cell lines by Stacey, G N

“…Standardisation of cell-based assays is significantly enhanced through the preparation and quality control of master and working cell banks. Validation of the…”
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An integrated strategy for the process development of a recombinant antibody-cytokine fusion protein expressed in BHK cells by BURGER, C, CARRONDO, M. J. T, MOREIRA, J.-L, RIEKE, E, SAVAGE, A. V, STACEY, G. N, WELGE, T, CRUZ, H, CUFFE, M, DIAS, E, GRIFFITHS, J. B, HAYES, K, HAUSER, H, LOOBY, D, MIELKE, C

“…Recombinant fusion proteins offer important new therapeutic approaches for the future. This report describes the use of three different genetic strategies…”
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Standardization in animal cell technology by Stacey, G N

“…Continuous cell lines offer a level of reproducibility, and thus standardization, which cannot normally be achieved using primary cells. However, even with…”
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New cell substrates for in vitro evaluation of microcystin hepato-cytotoxicity by Battle, T., Touchard, C., Moulsdale, H.J., Dowsett, B., Stacey, G.N.

“…Algal toxins, as Microcystins released in water supplies, may represent a serious health hazard. The behaviour of primary hepatocytes was compared with that of…”
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Hybridoma cell cultures continuously undergo apoptosis and reveal a novel 100 bp DNA fragment by Solis-Recendez, M.G., Perani, A., D'habit, B., Stacey, G.N., Maugras, M.

“…This report represents an investigation into the nature of apoptosis in hybridoma cultures and its significance to their utilization in biotechnology. To this…”
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DNA fingerprinting transforms the art of cell authentication by Stacey, Glyn N, Bolton, Bryan J, Doyle, Alan

“…The increasing diversity of new cell cultures is seriously stretching the capabilities of traditional methods of identification. DNA fingerprinting is set to…”
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Authentication of Animal Cell Cultures by Direct Visualization of Repetitive DNA, Aldolase Gene PCR and Isoenzyme Analysis by Stacey, G.N., Hoelzl, H., Stephenson, J.R., Doyle, A.

“…In studies utilizing animal cells as biological substrates, the authenticity of the cells used is critical to the validity of the derived experimental data…”
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Phenotypic and genotypic heterogeneity of peripheral T-cell lymphoma by SMITH, J. L, HAEGERT, D. G, HODGES, E, STACEY, G. N, HOWELL, W. M, WRIGHT, D. H, JONES, D. B

“…A series of 21 phenotypically characterised T-cell lymphomas histologically defined as lymphocytic, lymphoblastic, immunoblastic, AILD type, pleomorphic,…”
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Clonality of T cell and phenotypically undefined lymphoid neoplasms: the value of genotypic analyses by Hodges, E, Stacey, G N, Howell, W M, Jones, D B, Smith, J L

“…The value of genotypic analysis for routine assessment of leukaemia and lymphoma was shown by the findings in a selected series of 30 cases. T cell receptor…”
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Selection of host cell lines for scale-up growth conditions of a recombinant baculovirus vector expressing the measles virus nucleocapsid protein by HUTCHINSON, I, FOOKS, A. R, SMITH, R, STACEY, G. N

“…Various insect cell lines were grown as suspension cultures in spinner vessels and infected with a recombinant baculovirus vector expressing the measles virus…”
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Multilocus DNA fingerprint analysis of cellbanks: stability studies and culture identification in human B-lymphoblastoid and mammalian cell lines by STACEY, G. N, BOLTON, B. J, MORGAN, D, CLARK, S. A, DOYLE, A

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Quality assurance for cell substrates by Stacey, G N, Phillips, P

“…Animal cell lines are increasingly used in the manufacture of viral vaccines. They may play a key role in the preparation of seed stock virus and vaccine…”
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Host cells and cell banking by Stacey, Glyn N, Merten, Otto-Wilhelm

“…Gene therapy based on the use of viral vectors is entirely dependent on the use of animal cell lines, mainly of mammalian origin, but also of insect origin. As…”
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The quality control of cell banks using DNA fingerprinting by Stacey, G N, Bolton, B J, Doyle, A

“…Reproducibility in animal cell culture technology requires careful preparation and characterisation of banks of cell cultures. The two standard techniques used…”
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Neurological gene therapy by FOOKS, A.R., JENNINGS, A.D., DOYLE, A., CLEGG, J.C.S., STACEY, G.N.

“…Gene therapy applied to neurodegenerative disorders such as Alzheimer's and Parkinson's disease could become a reality, though ethical issues could delay…”
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Have bio-safety issues in cell culture been overlooked? by Stacey, G N, Sheeley, H J

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