Search Results - "Staals, Raymond H.J."

Structure and Activity of the RNA-Targeting Type III-B CRISPR-Cas Complex of Thermus thermophilus by Staals, Raymond H.J., Agari, Yoshihiro, Maki-Yonekura, Saori, Zhu, Yifan, Taylor, David W., van Duijn, Esther, Barendregt, Arjan, Vlot, Marnix, Koehorst, Jasper J., Sakamoto, Keiko, Masuda, Akiko, Dohmae, Naoshi, Schaap, Peter J., Doudna, Jennifer A., Heck, Albert J.R., Yonekura, Koji, van der Oost, John, Shinkai, Akeo

“…The CRISPR-Cas system is a prokaryotic host defense system against genetic elements. The Type III-B CRISPR-Cas system of the bacterium Thermus thermophilus,…”
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RNA Targeting by the Type III-A CRISPR-Cas Csm Complex of Thermus thermophilus by Staals, Raymond H.J., Zhu, Yifan, Taylor, David W., Kornfeld, Jack E., Sharma, Kundan, Barendregt, Arjan, Koehorst, Jasper J., Vlot, Marnix, Neupane, Nirajan, Varossieau, Koen, Sakamoto, Keiko, Suzuki, Takehiro, Dohmae, Naoshi, Yokoyama, Shigeyuki, Schaap, Peter J., Urlaub, Henning, Heck, Albert J.R., Nogales, Eva, Doudna, Jennifer A., Shinkai, Akeo, van der Oost, John

“…CRISPR-Cas is a prokaryotic adaptive immune system that provides sequence-specific defense against foreign nucleic acids. Here we report the structure and…”
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CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3 by Westra, Edze R., van Erp, Paul B.G., Künne, Tim, Wong, Shi Pey, Staals, Raymond H.J., Seegers, Christel L.C., Bollen, Sander, Jore, Matthijs M., Semenova, Ekaterina, Severinov, Konstantin, de Vos, Willem M., Dame, Remus T., de Vries, Renko, Brouns, Stan J.J., van der Oost, John

“…The prokaryotic CRISPR/Cas immune system is based on genomic loci that contain incorporated sequence tags from viruses and plasmids. Using small guide RNA…”
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Shooting the messenger: RNA-targetting CRISPR-Cas systems by Zhu, Yifan, Klompe, Sanne E, Vlot, Marnix, van der Oost, John, Staals, Raymond H J

“…Since the discovery of CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-associated genes) immune systems, astonishing progress has…”
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Structural plasticity and in vivo activity of Cas1 from the type I-F CRISPR-Cas system by Wilkinson, Max E, Nakatani, Yoshio, Staals, Raymond H J, Kieper, Sebastian N, Opel-Reading, Helen K, McKenzie, Rebecca E, Fineran, Peter C, Krause, Kurt L

“…CRISPR-Cas systems are adaptive immune systems in prokaryotes that provide protection against viruses and other foreign DNA. In the adaptation stage, foreign…”
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CRISPRDetect: A flexible algorithm to define CRISPR arrays by Biswas, Ambarish, Staals, Raymond H J, Morales, Sergio E, Fineran, Peter C, Brown, Chris M

“…CRISPR (clustered regularly interspaced short palindromic repeats) RNAs provide the specificity for noncoding RNA-guided adaptive immune defence systems in…”
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Keeping crispr in check: diverse mechanisms of phage-encoded anti-crisprs by Trasanidou, Despoina, Gerós, Ana Sousa, Mohanraju, Prarthana, Nieuwenweg, Anna Cornelia, Nobrega, Franklin L, Staals, Raymond H J

“…CRISPR-Cas represents the only adaptive immune system of prokaryotes known to date. These immune systems are widespread among bacteria and archaea, and provide…”
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Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR–Cas system by Staals, Raymond H. J., Jackson, Simon A., Biswas, Ambarish, Brouns, Stan J. J., Brown, Chris M., Fineran, Peter C.

“…CRISPR–Cas systems provide bacteria with adaptive immunity against foreign nucleic acids by acquiring short, invader-derived sequences called spacers. Here, we…”
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CRISPR-Cas-Mediated Phage Resistance Enhances Horizontal Gene Transfer by Transduction by Watson, Bridget N J, Staals, Raymond H J, Fineran, Peter C

“…A powerful contributor to prokaryotic evolution is horizontal gene transfer (HGT) through transformation, conjugation, and transduction, which can be…”
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The CRISPRs, They Are A-Changin': How Prokaryotes Generate Adaptive Immunity by Westra, Edze R, Swarts, Daan C, Staals, Raymond H.J, Jore, Matthijs M, Brouns, Stan J.J, van der Oost, John

“…All organisms need to continuously adapt to changes in their environment. Through horizontal gene transfer, bacteria and archaea can rapidly acquire new traits…”
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Bioinformatic evidence of widespread priming in type I and II CRISPR-Cas systems by Nicholson, Thomas J., Jackson, Simon A., Croft, Bradley I., Staals, Raymond H.J., Fineran, Peter C., Brown, Chris M.

“…CRISPR-Cas systems provide bacteria and archaea with adaptive immunity against invading genetic elements, such as plasmids, bacteriophages and archaeal…”
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Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility by Astuti, Dewi, Morris, Mark R, Cooper, Wendy N, Staals, Raymond H J, Wake, Naomi C, Fews, Graham A, Gill, Harmeet, Gentle, Dean, Shuib, Salwati, Ricketts, Christopher J, Cole, Trevor, van Essen, Anthonie J, van Lingen, Richard A, Neri, Giovanni, Opitz, John M, Rump, Patrick, Stolte-Dijkstra, Irene, Müller, Ferenc, Pruijn, Ger J M, Latif, Farida, Maher, Eamonn R

“…Eamonn Maher and colleagues report germline mutations in DIS3L2 causing the Perlman syndrome of overgrowth and susceptibility to Wilms tumor. DIS3L2 encodes a…”
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Analysis of protein–RNA interactions in CRISPR proteins and effector complexes by UV-induced cross-linking and mass spectrometry by Sharma, Kundan, Hrle, Ajla, Kramer, Katharina, Sachsenberg, Timo, Staals, Raymond H.J., Randau, Lennart, Marchfelder, Anita, van der Oost, John, Kohlbacher, Oliver, Conti, Elena, Urlaub, Henning

“…•Investigation of protein–RNA interaction sites by UV cross-linking and mass spectrometry.•Identification of RNA binding sites in RNA binding…”
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Priming in the Type I-F CRISPR-Cas system triggers strand-independent spacer acquisition, bi-directionally from the primed protospacer by Richter, Corinna, Dy, Ron L., McKenzie, Rebecca E., Watson, Bridget N.J., Taylor, Corinda, Chang, James T., McNeil, Matthew B., Staals, Raymond H.J., Fineran, Peter C.

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Structure of a type IV CRISPR-Cas ribonucleoprotein complex by Zhou, Yi, Bravo, Jack P.K., Taylor, Hannah N., Steens, Jurre A., Jackson, Ryan N., Staals, Raymond H.J., Taylor, David W.

“…We reveal the cryo-electron microscopy structure of a type IV-B CRISPR ribonucleoprotein (RNP) complex (Csf) at 3.9-Å resolution. The complex best resembles…”
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Ethylene regulates fast apoplastic acidification and expansin A transcription during submergence-induced petiole elongation in Rumex palustris by Vreeburg, R.A.M, Benschop, J.J, Peeters, A.J.M, Colmer, T.D, Ammerlaan, A.H.M, Staal, M, Elzenga, T.M, Staals, R.H.J, Darley, C.P, McQueen-Mason, S.J

“…The semi-aquatic dicot Rumex palustris responds to complete submergence by enhanced elongation of young petioles. This elongation of petiole cells brings leaf…”
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Alternative functions of CRISPR–Cas systems in the evolutionary arms race by Mohanraju, Prarthana, Saha, Chinmoy, van Baarlen, Peter, Louwen, Rogier, Staals, Raymond H. J., van der Oost, John

“…CRISPR–Cas systems of bacteria and archaea comprise chromosomal loci with typical repetitive clusters and associated genes encoding a range of Cas proteins…”
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Quorum Sensing Controls Adaptive Immunity through the Regulation of Multiple CRISPR-Cas Systems by Patterson, Adrian G., Jackson, Simon A., Taylor, Corinda, Evans, Gary B., Salmond, George P.C., Przybilski, Rita, Staals, Raymond H.J., Fineran, Peter C.

“…Bacteria commonly exist in high cell density populations, making them prone to viral predation and horizontal gene transfer (HGT) through transformation and…”
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Coevolution between bacterial CRISPR-Cas systems and their bacteriophages by Watson, Bridget N J, Steens, Jurre A, Staals, Raymond H J, Westra, Edze R, van Houte, Stineke

“…CRISPR-Cas systems provide bacteria and archaea with adaptive, heritable immunity against their viruses (bacteriophages and phages) and other parasitic genetic…”
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The diverse arsenal of type III CRISPR-Cas-associated CARF and SAVED effectors by Steens, Jurre A, Salazar, Carl Raymund P, Staals, Raymond H J

“…Type III CRISPR-Cas systems make use of a multi-subunit effector complex to target foreign (m)RNA transcripts complementary to the guide/CRISPR RNA (crRNA)…”
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