Search Results - "St.Onge, Robert P."

Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design by Smith, Justin D, Suresh, Sundari, Schlecht, Ulrich, Wu, Manhong, Wagih, Omar, Peltz, Gary, Davis, Ronald W, Steinmetz, Lars M, Parts, Leopold, St Onge, Robert P

“…Genome-scale CRISPR interference (CRISPRi) has been used in human cell lines; however, the features of effective guide RNAs (gRNAs) in different organisms have…”
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Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes by Hillenmeyer, Maureen E, Fung, Eula, Wildenhain, Jan, Pierce, Sarah E, Hoon, Shawn, Lee, William, Proctor, Michael, St.Onge, Robert P, Tyers, Mike, Koller, Daphne, Altman, Russ B, Davis, Ronald W, Nislow, Corey, Giaever, Guri

“…Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes (~80%) has no obvious…”
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Defining genetic interaction by Mani, Ramamurthy, St.Onge, Robert P, Hartman, John L. IV, Giaever, Guri, Roth, Frederick P

“…Sometimes mutations in two genes produce a phenotype that is surprising in light of each mutation's individual effects. This phenomenon, which defines genetic…”
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Quantitative analysis of protein interaction network dynamics in yeast by Celaj, Albi, Schlecht, Ulrich, Smith, Justin D, Xu, Weihong, Suresh, Sundari, Miranda, Molly, Aparicio, Ana Maria, Proctor, Michael, Davis, Ronald W, Roth, Frederick P, St.Onge, Robert P

“…Many cellular functions are mediated by protein–protein interaction networks, which are environment dependent. However, systematic measurement of interactions…”
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A method for high‐throughput production of sequence‐verified DNA libraries and strain collections by Smith, Justin D, Schlecht, Ulrich, Xu, Weihong, Suresh, Sundari, Horecka, Joe, Proctor, Michael J, Aiyar, Raeka S, Bennett, Richard A O, Chu, Angela, Li, Yong Fuga, Roy, Kevin, Davis, Ronald W, Steinmetz, Lars M, Hyman, Richard W, Levy, Sasha F, St.Onge, Robert P

“…The low costs of array‐synthesized oligonucleotide libraries are empowering rapid advances in quantitative and synthetic biology. However, high synthesis error…”
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Multiplex Identification of Microbes by Hyman, Richard W, St.Onge, Robert P, Allen, Edward A, Miranda, Molly, Aparicio, Ana Maria, Fukushima, Marilyn, Davis, Ronald W

“…We have adapted molecular inversion probe technology to identify microbes in a highly multiplexed procedure. This procedure does not require growth of the…”
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Mapping the Cellular Response to Small Molecules Using Chemogenomic Fitness Signatures by Lee, Anna Y., St.Onge, Robert P., Proctor, Michael J., Wallace, Iain M., Nile, Aaron H., Spagnuolo, Paul A., Jitkova, Yulia, Gronda, Marcela, Wu, Yan, Kim, Moshe K., Cheung-Ong, Kahlin, Torres, Nikko P., Spear, Eric D., Han, Mitchell K. L., Schlecht, Ulrich, Suresh, Sundari, Duby, Geoffrey, Heisler, Lawrence E., Surendra, Anuradha, Fung, Eula, Urbanus, Malene L., Gebbia, Marinella, Lissina, Elena, Miranda, Molly, Chiang, Jennifer H., Aparicio, Ana Maria, Zeghouf, Mahel, Davis, Ronald W., Cherfils, Jacqueline, Boutry, Marc, Kaiser, Chris A., Cummins, Carolyn L., Trimble, William S., Brown, Grant W., Schimmer, Aaron D., Bankaitis, Vytas A., Nislow, Corey, Bader, Gary D., Giaever, Guri

“…Genome-wide characterization of the in vivo cellular response to perturbation is fundamental to understanding how cells survive stress. Identifying the…”
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Improved discovery of genetic interactions using CRISPRiSeq across multiple environments by Jaffe, Mia, Dziulko, Adam, Smith, Justin D, St Onge, Robert P, Levy, Sasha F, Sherlock, Gavin

“…Large-scale genetic interaction (GI) screens in yeast have been invaluable for our understanding of molecular systems biology and for characterizing novel gene…”
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Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples by Smith, Andrew M, Heisler, Lawrence E, St.Onge, Robert P, Farias-Hesson, Eveline, Wallace, Iain M, Bodeau, John, Harris, Adam N, Perry, Kathleen M, Giaever, Guri, Pourmand, Nader, Nislow, Corey

“…Next-generation sequencing has proven an extremely effective technology for molecular counting applications where the number of sequence reads provides a…”
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A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions by Schlecht, Ulrich, Liu, Zhimin, Blundell, Jamie R., St.Onge, Robert P., Levy, Sasha F.

“…Several large-scale efforts have systematically catalogued protein-protein interactions (PPIs) of a cell in a single environment. However, little is known…”
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Yeast chemical genomics and drug discovery: an update by Hoon, Shawn, St.Onge, Robert P, Giaever, Guri, Nislow, Corey

“…The Saccharomyces cerevisiae sequencing project (the first eukaryotic genome decoded) was completed in 1995 and, subsequently, the first version of the yeast…”
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Multiplex assay for condition-dependent changes in protein–protein interactions by Schlecht, Ulrich, Miranda, Molly, Suresh, Sundari, Davis, Ronald W., St.Onge, Robert P.

“…Changes in protein–protein interactions that occur in response to environmental cues are difficult to uncover and have been poorly characterized to date. Here…”
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Exploring gene function and drug action using chemogenomic dosage assays by Ericson, Elke, Hoon, Shawn, St Onge, Robert P, Giaever, Guri, Nislow, Corey

“…In this chapter, we describe a series of genome-wide, cell-based assays that provide a solid basis for understanding drug-gene interactions, gene function, and…”
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Systematic Mapping of Chemical-Genetic Interactions in Saccharomyces cerevisiae by Suresh, Sundari, Schlecht, Ulrich, Xu, Weihong, Bray, Walter, Miranda, Molly, Davis, Ronald W, Nislow, Corey, Giaever, Guri, Lokey, R Scott, St Onge, Robert P

“…Chemical-genetic interactions (CGIs) describe a phenomenon where the effects of a chemical compound (i.e., a small molecule) on cell growth are dependent on a…”
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Identification of Chemical-Genetic Interactions via Parallel Analysis of Barcoded Yeast Strains by Suresh, Sundari, Schlecht, Ulrich, Xu, Weihong, Miranda, Molly, Davis, Ronald W, Nislow, Corey, Giaever, Guri, St Onge, Robert P

“…The Yeast Knockout Collection is a complete set of gene deletion strains for the budding yeast, Saccharomyces cerevisiae In each strain, one of approximately…”
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A Role for the Phosphorylation of hRad9 in Checkpoint Signaling by St.Onge, Robert P., Besley, Blair D.A., Pelley, Jennifer L., Davey, Scott

“…The integrity of the human genome is preserved by signal transduction pathways called checkpoints, which delay progression through the cell cycle when DNA…”
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Genome-wide requirements for resistance to functionally distinct DNA-damaging agents by Lee, William, St Onge, Robert P, Proctor, Michael, Flaherty, Patrick, Jordan, Michael I, Arkin, Adam P, Davis, Ronald W, Nislow, Corey, Giaever, Guri

“…The mechanistic and therapeutic differences in the cellular response to DNA-damaging compounds are not completely understood, despite intense study. To expand…”
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DNA Damage-dependent and -independent Phosphorylation of the hRad9 Checkpoint Protein by St.Onge, Robert P., Besley, Blair D.A., Park, Minwoo, Casselman, Richard, Davey, Scott

“…Cell cycle checkpoints are regulatory mechanisms that maintain genomic integrity by preventing cell cycle progression when genetic anomalies are present. The…”
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Abstract 1604: Highly sensitive digital detection of circulating DNA cancer mutations using synthetic genome standards by Wood-Bouwens, Christina M., St.Onge, Robert P., Ji, Hanlee P.

“…Abstract With the emergence of precision cancer medicine, there is an increasing need for new longitudinal diagnostic tests to evaluate patients at the initial…”
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Multiplexed precision genome editing with trackable genomic barcodes in yeast by Roy, Kevin R, Smith, Justin D, Vonesch, Sibylle C, Lin, Gen, Tu, Chelsea Szu, Lederer, Alex R, Chu, Angela, Suresh, Sundari, Nguyen, Michelle, Horecka, Joe, Tripathi, Ashutosh, Burnett, Wallace T, Morgan, Maddison A, Schulz, Julia, Orsley, Kevin M, Wei, Wu, Aiyar, Raeka S, Davis, Ronald W, Bankaitis, Vytas A, Haber, James E, Salit, Marc L, St.Onge, Robert P, Steinmetz, Lars M

“…A method to introduce defined mutations into the yeast genome enables saturation mutagenesis of a gene and genome-scale introduction of genetic variants. Our…”
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