Prenatal and postnatal diagnoses of thalassemias and hemoglobinopathies by HPLC

The conventional approach to qualitative and quantitative analyses of hemoglobin (Hb) molecules for the diagnoses of hemoglobinopathies requires a combination of tests. We used an automated HPLC (VARIANT) system to study alpha-thalassemia and beta-thalassemia syndromes in Thailand. The beta-thalasse...

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Bibliographic Details
Published in:	Clinical chemistry (Baltimore, Md.) Vol. 44; no. 4; pp. 740 - 748
Main Authors:	Fucharoen, Suthat, Winichagoon, Pranee, Wisedpanichkij, Raewadee, Sae-Ngow, Busara, Sriphanich, Rungrat, Oncoung, Warangkana, Muangsapaya, Wanna, Chowthaworn, Jew, Kanokpongsakdi, Sujin, Bunyaratvej, Ahnond, Piankijagum, Anong, Dewaele, Chris
Format:	Journal Article
Language:	English
Published:	Washington, DC Am Assoc Clin Chem 01-04-1998 American Association for Clinical Chemistry
Subjects:	Adult alpha-Thalassemia - blood alpha-Thalassemia - diagnosis Anemias. Hemoglobinopathies beta-Thalassemia - blood beta-Thalassemia - diagnosis Biological and medical sciences Chromatography, High Pressure Liquid - methods Diseases of red blood cells Female Fetal Blood - chemistry Hematologic and hematopoietic diseases Hematology Hemoglobin A2 - analysis Hemoglobinopathies - blood Hemoglobinopathies - diagnosis Hemoglobins, Abnormal - analysis Humans Infant, Newborn Investigative techniques, diagnostic techniques (general aspects) Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Phenotype Pregnancy Prenatal Diagnosis - methods Thailand Thailand Asia South east Asia Human Hemoglobinopathy Biological fluid Automation HPLC chromatography Hemopathy Blood Genetic disease Prenatal Hemolytic anemia Postnatal β-Thalassemia α-Thalassemia Clinical biology Diagnosis
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Summary:	The conventional approach to qualitative and quantitative analyses of hemoglobin (Hb) molecules for the diagnoses of hemoglobinopathies requires a combination of tests. We used an automated HPLC (VARIANT) system to study alpha-thalassemia and beta-thalassemia syndromes in Thailand. The beta-thalassemia short program is applicable to the diagnosis of alpha-thalassemia and beta-thalassemia disorders, including Hb H, EA Bart's disease, and EF Bart's disease, in adults, newborns, and fetuses. The system cannot quantify accurately certain Hb molecules, such as Hb H and Hb Bart's. The alpha-thalassemia short program was therefore developed and used to quantify Hb Bart's to detect alpha-thalassemia genotypes in cord blood. This automated HPLC system is an alternative approach to the diagnosis of complicated thalassemia syndromes in Thailand and Southeast Asia.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0009-9147 1530-8561
DOI:	10.1093/clinchem/44.4.740