Cryopreserved human haematopoietic stem cells retain engraftment potential after extended (5–14 years) cryostorage

Harvesting of stem cells during the early phases of treatment with no immediate intention to perform a stem cell transplant is becoming an increasingly common practice. Such “insurance” harvests are often stored for many years before being needed for transplant in a subsequent relapse. The effect of...

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Bibliographic Details
Published in:	Cryobiology Vol. 44; no. 3; pp. 210 - 217
Main Authors:	Spurr, Elisabeth E, Wiggins, Nicole E, Marsden, Katherine A, Lowenthal, Raymond M, Ragg, Scott J
Format:	Journal Article
Language:	English
Published:	San Diego, CA Elsevier Inc 01-06-2002 Elsevier
Subjects:	Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Antigens, CD34 - metabolism Biological and medical sciences Bone Marrow Transplantation Bone marrow, stem cells transplantation. Graft versus host reaction Cell Survival Colony-Forming Units Assay Cryopreservation Engraftment potential Graft Survival Haematopoietic stem cells Hematopoietic Stem Cell Transplantation Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - immunology Humans In Vitro Techniques Long-term storage Medical sciences Time Factors Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transplantation, Autologous Haematopoietic stem cells Time factors Cryopreservation Long-term storage Engraftment potential Human Stem cell Hematopoietic cell Bone marrow Engraftment Long term Viability
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Summary:	Harvesting of stem cells during the early phases of treatment with no immediate intention to perform a stem cell transplant is becoming an increasingly common practice. Such “insurance” harvests are often stored for many years before being needed for transplant in a subsequent relapse. The effect of long-term cryostorage (5–14 years) on the viability and functional capacity of haematopoietic stem cells (HSCs) was investigated in 40 bone marrow and peripheral blood harvests using standard in vitro methods, the colony forming unit-granulocyte/macrophage (CFU-GM) assay and a single platform viable CD34 + cell absolute count by flow cytometry. Forty percent of harvests had CD34 + HSC counts of at least 0.7×10 6/kg bodyweight and 85% had CFU-GM counts of at least 1.0×10 5/kg bodyweight, these values representing our institutional minimum requirements for safe transplantation. Based on these results, it appears that HSC collections can remain adequate for safe transplantation after up to 14 years of cryostorage. However, as deterioration of HSC quality and viability may occur, some precautions may be warranted, namely harvesting higher than normal numbers of HSCs in collections intended for long-term storage and repeating in vitro assays on harvests after long-term storage prior to transplantation.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0011-2240 1090-2392
DOI:	10.1016/S0011-2240(02)00027-5