Search Results - "Springer, B. A."

High-Level Expression of Sperm Whale Myoglobin in Escherichia coli by Springer, Barry A., Sligar, Stephen G.

“…Sperm whale myoglobin was expressed in Escherichia coli from a totally synthetic gene inserted in the expression vector pUC19. The gene was constructed as 23…”
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Energetic Characterization of the Basic Fibroblast Growth Factor-Heparin Interaction: Identification of the Heparin Binding Domain by Thompson, Leo D, Pantoliano, Michael W, Springer, Barry A

“…Fibroblast growth factors (FGF's) interact on cell surfaces with "low-affinity" heparan sulfate proteoglycans (HSPG) and "high-affinity" FGF receptors (FGFR)…”
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Discrimination between oxygen and carbon monoxide and inhibition of autooxidation by myoglobin. Site-directed mutagenesis of the distal histidine by SPRINGER, B. A, EGEBERG, K. D, SLIGAR, S. G, ROHLFS, R. J, MATHEWS, A. J, OLSON, J. S

“…Sperm whale myoglobin mutants were constructed using site-directed mutagenesis to replace the highly conserved distal histidine residue (His(E7)-64). His-64…”
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The effects of amino acid substitution at position E7 (residue 64) on the kinetics of ligand binding to sperm whale myoglobin by Rohlfs, R J, Mathews, A J, Carver, T E, Olson, J S, Springer, B A, Egeberg, K D, Sligar, S G

“…Association and dissociation rate constants were measured for O2, CO, and alkyl isocyanide binding to a set of genetically engineered sperm whale myoglobins…”
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Analysis of the kinetic barriers for ligand binding to sperm whale myoglobin using site-directed mutagenesis and laser photolysis techniques by Carver, T E, Rohlfs, R J, Olson, J S, Gibson, Q H, Blackmore, R S, Springer, B A, Sligar, S G

“…Time courses for NO, O2, CO, methyl and ethyl isocyanide rebinding to native and mutant sperm whale myoglobins were measured at 20 degrees C following 17-ns…”
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Transient spectroscopy of the reaction of cyanide with ferrous myoglobin. Effect of distal side residues by Bellelli, A, Antonini, G, Brunori, M, Springer, B.A., Sligar, S.G.

“…The reaction of cyanide metmyoglobin with dithionite conforms to a two-step sequential mechanism with formation of an unstable intermediate, identified as…”
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Ligand binding to heme proteins: III. FTIR studies of His-E7 and Val-E11 mutants of carbonmonoxymyoglobin by Braunstein, D.P., Chu, K., Egeberg, K.D., Frauenfelder, H., Mourant, J.R., Nienhaus, G.U., Ormos, P., Sligar, S.G., Springer, B.A., Young, R.D.

“…Fouier-transform infrared (FTIR) difference spectra of several His-E7 and Val-E11 mutants of sperm whale carbonmonoxymyoglobin were obtained by…”
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The role of Val68(E11) in ligand binding to sperm whale myoglobin. Site-directed mutagenesis of a synthetic gene by EGEBERG, K. D, SPRINGER, B. A, SLIGAR, S. G, CARVER, T. E, ROHLFS, R. J, OLSON, J. S

“…Site-directed mutants of sperm whale myoglobin were prepared to probe the functional role of the highly conserved distal pocket valine residue, Val68(E11)…”
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The role of the distal histidine in myoglobin and haemoglobin by Olson, John S, Mathews, Antony J, Rohlfs, Ronald J, Springer, Barry A, Egeberg, Karen D, Sligar, Stephen G, Tame, Jeremy, Renaud, Jean-Paul, Nagai, Kiyoshi

“…The distal E7 histidine in vertebrate myoglobins and haemoglobins has been strongly conserved during evolution and is thought to be important in fine-tuning…”
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Resonance Raman studies of iron spin and axial coordination in distal pocket mutants of ferric myoglobin by MORIKIS, D, CHAMPION, P. M, SPRINGER, B. A, EGEBERG, K. D, SLIGAR, S. G

“…We have used resonance Raman spectroscopy to study 11 distal pocket mutants and the "wild type" and native ferric sperm whale myoglobin. The characteristic…”
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Crystal structure of myoglobin from a synthetic gene by Phillips, Jr, G N, Arduini, R M, Springer, B A, Sligar, S G

“…Crystals have been grown of myoglobin produced in Escherichia coli from a synthetic gene, and the structure has been solved to 1.9 A resolution. The space…”
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Alteration of sperm whale myoglobin heme axial ligation by site-directed mutagenesis by Egeberg, Karen D, Springer, Barry A, Martinis, Susan A, Sligar, Stephen G, Morikis, Dimitrios, Champion, Paul M

“…Three mutant proteins of sperm whale myoglobin (Mb) that exhibit altered axial ligations were constructed by site-directed mutagenesis of a synthetic gene for…”
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Site-Directed Mutagenesis of Histidine Residues Involved in Cu(II) Binding and Reduction by Sperm Whale Myoglobin by Van Dyke, B. R., Bakan, D. A., Glover, K. A. M., Hegenauer, J. C., Saltman, P., Springer, B. A., Sligar, S. G.

“…Sperm whale myoglobin (Mb) reduces Cu(II) through a site-specific mechanism involving complexation by one or more surface histidine residues. Three mutants of…”
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Identification and concerted function of two receptor binding surfaces on basic fibroblast growth factor required for mitogenesis by Springer, B A, Pantoliano, M W, Barbera, F A, Gunyuzlu, P L, Thompson, L D, Herblin, W F, Rosenfeld, S A, Book, G W

“…Members of the fibroblast growth factor (FGF) family promote angiogenesis and wound repair, modulate early developmental events and survival of neurons, and…”
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Ligand Binding to Synthetic Mutant Myoglobin (His-E7 → Gly): Role of the Distal Histidine by Braunstein, D., Ansari, A., Berendzen, J., Cowen, B. R., Egeberg, K. D., Frauenfelder, H., Hong, M. K., Ormos, P., Sauke, T. B., Scholl, R., Schulte, A., Sligar, S. G., Springer, B. A., Steinbach, P. J., Young, R. D.

“…Low-temperature flash photolysis with IR and visible spectroscopy was used to probe the influence of the distal histidine His-64(E7) of sperm-whale myoglobin…”
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Multivalent Ligand-Receptor Binding Interactions in the Fibroblast Growth Factor System Produce a Cooperative Growth Factor and Heparin Mechanism for Receptor Dimerization by Pantoliano, Michael W, Horlick, Robert A, Springer, Barry A, Van Dyk, Drew E, Tobery, Timothy, Wetmore, Diana R, Lear, James D, Nahapetian, Ara T, Bradley, Jodi D, Sisk, William P

“…The binding interactions for the three primary reactants of the fibroblast growth factor (FGF) system, basic FGF (bFGF), an FGF receptor, FGFR1, and the…”
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High-density miniaturized thermal shift assays as a general strategy for drug discovery by Pantoliano, M W, Petrella, E C, Kwasnoski, J D, Lobanov, V S, Myslik, J, Graf, E, Carver, T, Asel, E, Springer, B A, Lane, P, Salemme, F R

“…More general and universally applicable drug discovery assay technologies are needed in order to keep pace with the recent advances in combinatorial chemistry…”
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A Prospective Study of Modified Ottawa Ankle Rules in a Military Population: Interobserver Agreement Between Physical Therapists and Orthopaedic Surgeons by Springer, Barbara A., Arciero, Robert A., Tenuta, Joachim J., Taylor, Dean C.

“…To determine the necessity of ankle and foot radiographs, we used modified Ottawa Ankle Rules to evaluate all cadets seen with an acute ankle or midfoot injury…”
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Resonance Raman investigations of site-directed mutants of myoglobin: effects of distal histidine replacement by Morikis, Dimitrios, Champion, Paul M, Springer, Barry A, Sligar, Stephen G

“…The resonance Raman spectra of met-, deoxy-, and (carbonmonoxy)myoglobin (MbCO) are studied as a function of amino acid replacement at the distal histidine-E7…”
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Levels of Evidence in the Journal of Music Therapy from 2000-2009: Descriptive Analyses by Year and Clinical Population by Yun-Springer, A. B., Silverman, M. J.

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