Search Results - "Spitsyn, M A"

Infrared fluorescent markers for microarray DNA analysis by Spitsyn, M. A., Shershov, V. E., Kuznetsova, V. E., Barsky, V. E., Egorov, E. E., Emelyanova, M. A., Kreindlin, E. Ya, Lysov, Yu. P., Guseinov, T. O., Fesenko, D. E., Lapa, S. A., Surzhikov, S. A., Abramov, I. S., Nasedkina, T. V., Zasedatelev, A. S., Chudinov, A. V.

“…In order to expand the informational capabilities of molecular genetic research, new indotricarbocyanine dyes that fluoresce in the near infrared (IR) spectral…”
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Comparative Study of Novel Fluorescent Cyanine Nucleotides: Hybridization Analysis of Labeled PCR Products Using a Biochip by Shershov, V. E., Lapa, S. A., Kuznetsova, V. E., Spitsyn, M. A., Guseinov, T. O., Polyakov, S. A., Stomahin, A. A., Zasedatelev, A. S., Chudinov, A. V.

“…This study investigated the synthesis and substrate properties of Cy5-labeled dUTP derivatives with different substituents, linkers between the dye unit and…”
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Preparation of Modified Combinatorial DNA Libraries via Emulsion PCR with Subsequent Strand Separation by Lapa, S. A., Romashova, K. S., Spitsyn, M. A., Shershov, V. E., Kuznetsova, V. E., Guseinov, T. O., Zasedateleva, O. A., Radko, S. P., Timofeev, E. N., Lisitsa, A. V., Chudinov, A. V.

“…— A modification of the enzymatic method for the preparation of combinatorial random DNA libraries, which combines amplification in isolated microvolumes with…”
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Enzymatic Preparation of Modified DNA: Study of the Kinetics by Real-Time PCR by Lapa, S. A., Pavlov, A. S., Kuznetsova, V. E., Shershov, V. E., Spitsyn, M. A., Guseinov, T. O., Radko, S. P., Zasedatelev, A. S., Lisitsa, A. V., Chudinov, A. V.

“…The effects of modified deoxyuridine triphosphates (mod-dUTPs) with different substituents at the C5 position of the pyrimidine cycle on the kinetics of PCR…”
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Amplification Efficiency and Substrate Properties of Fluorescently Labeled Deoxyuridine Triphosphates in PCR in the Presence of DNA Polymerases without 3'-5' Exonuclease Activity by Lapa, S. A., Volkova, O. S., Spitsyn, M. A., Shershov, V. E., Kuznetsova, V. E., Guseinov, T. O., Zasedatelev, A. S., Chudinov, A. V.

“…The kinetics of PCR amplification has been studied using fluorescently labeled zwitterionic deoxyuridine triphosphates that contain different fluorophores…”
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Fluorescent Labeling of Oligonucleotide Probes for Double Indicator Microarray Hybridization Analysis by Shershov, V. E., Miftakhov, R. A., Kuznetsova, V. E., Timofeev, E. N., Grechishnikova, I. V., Spitsyn, M. A., Guseinov, T. O., Lapa, S. A., Zasedatelev, A. S., Chudinov, A. V.

“…Herein, we present a synthesis of a molecular construct based on the polysulfonated indocarbocyanine dye and the lysine. The chemical structure of the…”
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Substrate Properties of New Fluorescently Labeled Deoxycytidine Triphosphates in Enzymatic Synthesis of DNA with Polymerases of Families A and B by Fesenko, D. O., Guseinov, T. O., Lapa, S. A., Kuznetsova, V. E., Shershov, V. E., Spitsyn, M. A., Nasedkina, T. V., Zasedatelev, A. S., Chudinov, A. V.

“…The efficiency of the incorporation of fluorescently labeled derivatives of 2'-deoxycytidine in DNA synthesized de novo has been studied using PCR with Taq and…”
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Novel 5-Alkylcarboxamide-2'-Deoxyuridine-5'-Triphosphates for Enzymatic Synthesis of Highly Modified DNA by Vasiliskov, V. A., Lapa, S. A., Kuznetsova, V. E., Surzhikov, S. A., Shershov, V. E., Spitsyn, M. A., Guseinov, T. O., Miftahov, R. A., Zasedateleva, O. A., Lisitsa, A. V., Radko, S. P., Zasedatelev, A. S., Timofeev, E. N., Chudinov, A. V.

“…The synthesis of deoxyuridine triphosphate derivatives with alkyl groups of various chain length attached to the pyrimidine base by the trans -alkene linker…”
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Substrate Properties of New Fluorescently Labeled Deoxycytidine Triphosphates in Enzymatic Synthesis of DNA with Polymerases of Families A and B by Fesenko, D O, Guseinov, T O, Lapa, S A, Kuznetsova, V E, Shershov, V E, Spitsyn, M A, Nasedkina, T V, Zasedatelev, A S, Chudinov, A V

“…The efficiency of the incorporation of fluorescently labeled derivatives of 2'-deoxycytidine in DNA synthesized de novo has been studied using PCR with Taq and…”
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Structural and functional analysis of biopolymers and their complexes: Enzymatic synthesis of high-modified DNA by Chudinov, A. V., Kiseleva, Y. Y., Kuznetsov, V. E., Shershov, V. E., Spitsyn, M. A., Guseinov, T. O., Lapa, S. A., Timofeev, E. N., Archakov, A. I., Lisitsa, A. V., Radko, S. P., Zasedatelev, A. S.

“…Here, we describe the synthesis and purification of six deoxyuridine triphosphate derivatives that contain protein-like functional groups and alkene linkers of…”
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New Synthetic Route to CY5-Labeled 2'-Deoxycytidine- 5'-Triphosphates Using Sonogashira Reaction by Guseinov, T. O., Kuznetsova, V. E., Shershov, V. E., Spitsyn, M. A., Lapa, S. A., Zasedatelev, A. S., Chudinov, A. V.

“…Herein, we present a new synthetic route to fluorescently-labeled nucleoside triphosphates via Sonogashira cross-coupling of iodinated deoxycytidine…”
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Cyanine-dye-modified 2′-deoxyuridine-5′-triphosphates: Synthesis, applications, and linker effect on substrate properties for Taq DNA polymerase by Spitsyn, M. A., Kuznetsova, V. E., Shershov, V. E., Lapa, S. A., Guseinov, T. O., Zasedatelev, A. S., Chudinov, A. V.

“…A number of fluorescently labeled nucleoside triphosphates containing electroneutral indodicarbocyanine dye (Cy) have been synthesized. The dye has been…”
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The Effect of the Chromophore Charge on the Efficiency of Incorporation of Fluorescently-labeled Nucleotides in Matrix Synthesis by Taq DNA Polymerase by Shershov, V E, Kuznetsova, V E, Lysov, Yu P, Guseinov, T O, Barsky, V E, Spitsyn, M A, Zasedateleva, O A, Vasiliskov, V A, Surzhikov, S A, Zasedatelev, A S, Chudinov, A V

“…In order to study the effect of an electrical charge of the chromophore, on the efficiency of incorporation of fluorescently-labeled nucleotides into DNA…”
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Generation of Active Carboxyl Groups on the Surface of a Polyethylene Terephthalate Film and Their Quantitation by Digital Fluorescence Microscopy by Miftakhov, R. A., Lapa, S. A., Shershov, V. E., Zasedateleva, O. A., Guseinov, T. O., Spitsyn, M. A., Kuznetsova, V. E., Mamaev, D. D., Lysov, Yu. P., Barsky, V. E., Timofeev, E. N., Zasedatelev, A. S., Chudinov, A. V.

“…A method to estimate the concentration of chemically accessible carboxylic groups on the surface of a polyethylene terephthalate (PET) film was developed using…”
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The Effect of the Structure of Fluorescently Labeled Nucleotide Derivatives on the Efficiency of Their Incorporation in DNA in the Polymerase Chain Reaction by Ikonnikova, A. Yu, Lisitsa, T. S., Shershov, V. E., Spitsyn, M. A., Guseinov, T. O., Fesenko, D. O., Lapa, S. A., Kuznetsova, V. E., Zasedatelev, A. S., Chudinov, A. V., Nasedkina, T. V.

“…The efficiency of fluorescence DNA labeling was estimated for four fluorescent 2′-deoxyuridine 5′-triphosphate derivatives differing in the orientation of the…”
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The kinetics of fluorescent DNA labeling using PCR with different Taq polymerases depends on the chemical structures of modified nucleotides by Lisitsa, T. S., Shershov, V. E., Spitsyn, M. A., Guseinov, T. O., Ikonnikova, A. Yu, Fesenko, D. O., Lapa, S. A., Zasedatelev, A. S., Chudinov, A. V., Nasedkina, T. V.

“…The kinetics of DNA labeling during PCR using six fluorescent derivatives of 2′-deoxyuridine 5′-triphosphate has been studied. These compounds differ in their…”
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The effect of chromophore charge on the incorporation efficiency of fluorescence-labeled nucleotides catalyzed by Taq DNA polymerase in matrix synthesis by Shershov, V. E., Kuznetsova, V. E., Lysov, Yu. P., Guseinov, T. O., Barsky, V. E., Spitsyn, M. A., Zasedateleva, O. A., Vasiliskov, V. A., Surzhikov, S. A., Zasedatelev, A. S., Chudinov, A. V.

“…The effect of the chromophore charge on the efficiency of incorporation of fluorescent-labeled nucleotides into DNA during PCR was studied using three dUTP…”
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Study of the polyaniline film degradation process by radiotracer and electrochemical methods by Kazarinov, V.E., Andreev, V.N., Spitsyn, M.A.

“…The electro-oxidative degradation of polyaniline films electrodeposited on the platinum electrode has been studied using radiotracer and potentiodynamic pulse…”
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Preparation of Modified Combinatorial DNA Libraries via Emulsion PCR with Subsequent Strand Separation by Lapa, S A, Romashova, K S, Spitsyn, M A, Shershov, V E, Kuznetsova, V E, Guseinov, T O, Zasedateleva, O A, Radko, S P, Timofeev, E N, Lisitsa, A V, Chudinov, A V

“…A modification of the enzymatic method for the preparation of combinatorial random DNA libraries, which combines amplification in isolated microvolumes with…”
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Enzymatic synthesis of high-modified DNA by Chudinov, A V, Kiseleva, Y Y, Kuznetsov, V E, Shershov, V E, Spitsyn, M A, Guseinov, T O, Lapa, S A, Timofeev, E N, Archakov, A I, Lisitsa, A V, Radko, S P, Zasedatelev, A S

“…Here, we describe the synthesis and purification of six deoxyuridine triphosphate derivatives that contain protein-like functional groups and alkene linkers of…”
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