Sequence-specific binding of Taz1p dimers to fission yeast telomeric DNA

The fission yeast (Schizosaccharomyces pombe) taz1 gene encodes a telomere-associated protein. It contains a single copy of a Myb-like motif termed the telobox that is also found in the human telomere binding proteins TRF1 and TRF2, and Tbf1p, a protein that binds to sequences found within the sub-t...

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Bibliographic Details
Published in:	Nucleic acids research Vol. 28; no. 2; pp. 527 - 533
Main Authors:	Spink, K.G, Evans, R.J, Chambers, A
Format:	Journal Article
Language:	English
Published:	England Oxford Publishing Limited (England) 15-01-2000 Oxford University Press
Subjects:	Amino Acid Sequence Bacterial Outer Membrane Proteins - chemistry Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - metabolism Base Sequence binding Cloning, Molecular Dimerization DNA DNA Primers DNA, Fungal - metabolism DNA-binding domains DNA-binding proteins Escherichia coli Proteins genes Humans Molecular Sequence Data nucleotide sequences Phosphoprotein Phosphatases Protein Binding Protein Kinases Proto-Oncogene Proteins c-myb - metabolism Saccharomyces cerevisiae Schizosaccharomyces - genetics Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid taz1 gene Taz1 protein Tbf1 protein Telomere telomere-associated protein telomere-binding protein Telomere-Binding Proteins telomeres TRF1 protein TRF2 protein Two-Hybrid System Techniques
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Summary:	The fission yeast (Schizosaccharomyces pombe) taz1 gene encodes a telomere-associated protein. It contains a single copy of a Myb-like motif termed the telobox that is also found in the human telomere binding proteins TRF1 and TRF2, and Tbf1p, a protein that binds to sequences found within the sub-telomeric regions of budding yeast (Saccharomyces cerevisiae) chromosomes. Taz1p was synthesised in vitro and shown to bind to a fission yeast telomeric DNA fragment in a sequence specific manner that required the telobox motif. Like the mammalian TRF proteins, Taz1p bound to DNA as a preformed homodimer. The isolated Myb-like domain was also capable of sequence specific DNA binding, although with less specificity than the full-length dimer. Surprisingly, a protein extract produced from a taz1- fission yeast strain still contained the major telomere binding activity (complex I) we have characterised previously, suggesting that there could be other abundant telomere binding proteins in fission yeast. One candidate, SpX, was also synthesised in vitro, but despite the presence of two telobox domains, no sequence specific binding to telomeric DNA was detected.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 To whom correspondence should be addressed. Tel +44 115 970 9225; Fax: +44 115 970 9906; Email: alistair.chambers@nott.ac.uk
ISSN:	0305-1048 1362-4962 1362-4962
DOI:	10.1093/nar/28.2.527