Comparison of two analytical methods for detecting Clostridium spores in milk

A study involving analysis of raw milk samples naturally contaminated by Clostridium spores was organised and the analytical performance of AmpMedia 666 broth was evaluated in comparison with the conventional MPN method based on the use of reinforced clostridial medium (RCM) broth. The selectivity o...

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Bibliographic Details
Published in:	International dairy journal Vol. 142; p. 105649
Main Authors:	Andrighetto, Christian, Spolaor, Dino, De Dea, Paola, Fioravanzo, Elisa, Brazzale, Piercristiano, De Battista, Matteo, Dolci, Elisa, Maccà, Francesca, Sperotto, Davide, Lombardi, Angiolella
Format:	Journal Article
Language:	English
Published:	Elsevier Ltd 01-07-2023
Online Access:	Get full text
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Summary:	A study involving analysis of raw milk samples naturally contaminated by Clostridium spores was organised and the analytical performance of AmpMedia 666 broth was evaluated in comparison with the conventional MPN method based on the use of reinforced clostridial medium (RCM) broth. The selectivity of RCM medium in detecting Clostridium after 2 days, 5 days and 7 days of incubation at 37 °C was 88%, 78% and 65%, respectively, while the selectivity of AmpMedia 666 was 96%. Most of the colonies not belonging to the genus Clostridium were assigned to the spore forming genera Bacillus, Virgibacillus and Rummeliibacillus.
ISSN:	0958-6946 1879-0143
DOI:	10.1016/j.idairyj.2023.105649