Search Results - "Speer, Shannon L"

Macromolecular Crowding Is More than Hard-Core Repulsions by Speer, Shannon L, Stewart, Claire J, Sapir, Liel, Harries, Daniel, Pielak, Gary J

“…Cells are crowded, but proteins are almost always studied in dilute aqueous buffer. We review the experimental evidence that crowding affects the equilibrium…”
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The intracellular environment affects protein–protein interactions by Speer, Shannon L., Zheng, Wenwen, Jiang, Xin, Chu, I-Te, Guseman, Alex J., Liu, Maili, Pielak, Gary J., Li, Conggang

“…Protein–protein interactions are essential for life but rarely thermodynamically quantified in living cells. In vitro efforts show that protein complex…”
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Protein shape modulates crowding effects by Guseman, Alex J., Goncalves, Gerardo M. Perez, Speer, Shannon L., Young, Gregory B., Pielak, Gary J.

“…Protein−protein interactions are usually studied in dilute buffered solutions with macromolecule concentrations of <10 g/L. In cells, however, the…”
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Surface Charge Modulates Protein–Protein Interactions in Physiologically Relevant Environments by Guseman, Alex J, Speer, Shannon L, Perez Goncalves, Gerardo M, Pielak, Gary J

“…Protein–protein interactions are fundamental to biology yet are rarely studied under physiologically relevant conditions where the concentration of…”
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A Difference between In Vitro and In-Cell Protein Dimer Formation by Chu, I-Te, Stewart, Claire J, Speer, Shannon L, Pielak, Gary J

“…The high concentration of macromolecules in cells affects the stability of proteins and protein complexes via hard repulsions and chemical interactions, yet…”
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Rheostatic Control of Protein Expression Using Tuner Cells by Chu, I-Te, Speer, Shannon L, Pielak, Gary J

“…We assessed the ability of two strains of Escherichia coli, BL21 (DE3) and Tuner (DE3), to express a variant of the B1 domain of protein G, which forms a…”
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Controlling and quantifying protein concentration in Escherichia coli by Speer, Shannon L., Guseman, Alex J., Patteson, Jon B., Ehrmann, Brandie M., Pielak, Gary J.

“…The cellular environment is dynamic and complex, involving thousands of different macromolecules with total concentrations of hundreds of grams per liter…”
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Quantifying a Protein-Protein Interaction in Living Cells by Speer, Shannon L., Guseman, Alex J., Pielak, Gary J.

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Polyethylene Glycol Size and Protein-Complex Stability by Lauzier, Francis J., Stewart, Claire J., Harries, Daniel, Pielak, Gary J., Speer, Shannon L.

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Polyethylene Glycol Size and Protein Stability by Stewart, Claire J., Speer, Shannon L., Lauzier, Francis J., Harries, Daniel, Pielak, Gary J.

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Tardigrade Intrinsically Disordered Proteins Protect Enzymes from Desiccation-Induced Inactivation by Piszkiewicz, Samantha, Gunn, Kathryn H., Speer, Shannon L., Warmuth, Owen, Mehta, Aakash, Lauzier, Francis J., Nguyen, Kenny H., Kuhlman, Elizabeth, Neher, Saskia B., Pielak, Gary J.

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