Locus-specific de novo methylation down-regulates MHC class I in S49 lymphomas
To investigate mechanisms involved in the regulation of MHC class I expression, we studied a series of tumor variants that were originally isolated from the S49 BALB/c lymphoma. These S49 sublines express unusual combinations of their MHC class I molecules. This aberrant class I expression was found...
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| Published in: | Immunogenetics (New York) Vol. 43; no. 1-2; pp. 63 - 67 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
1995
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| Online Access: | Get full text |
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| Summary: | To investigate mechanisms involved in the regulation of MHC class I expression, we studied a series of tumor variants that were originally isolated from the S49 BALB/c lymphoma. These S49 sublines express unusual combinations of their MHC class I molecules. This aberrant class I expression was found to be stable during passage and to be non-reversible by treatment with gamma interferon. Four different class I phenotypes have been characterized at the molecular level: wild-type, two intermediates, and a null phenotype. These sublines provide a unique resource to define mechanisms involved with locus-specific regulation in a large multigene family. Extensive characterization of the class I genes in the S49 sublines indicated that aberrant H2 class I expression resulted from a cis-acting transcriptional defect. In the present study we characterized these S49 variants at the genomic level to elucidate potential mechanisms responsible for the locus-specific down-regulation of their class I genes. |
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| Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| ISSN: | 0093-7711 1432-1211 |
| DOI: | 10.1007/BF00186605 |