Search Results - "Spassky, Annick"

Chemical Probing Shows That the Intron-encoded Endonuclease I-SceI Distorts DNA through Binding in Monomeric Form to Its Homing Site by Beylot, B, Spassky, A

“…Despite its small size (27.6 kDa), the group I intron-encoded I- Sce I endonuclease initiates intron homing by recognizing and specifically cleaving a large…”
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Nuclease activity of 1,10-phenanthroline-copper ion. Conformational analysis and footprinting of the lac operon by Spassky, Annick, Sigman, David S

“…The nuclease activity of 1,10-phenanthroline-copper [(OP)2Cu+] preferentially nicks the wild-type, Ps, and L8-UV-5 lac promoters in the conserved promoter…”
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Chemical nuclease activity of 5-phenyl-1,10-phenanthroline-copper ion detects intermediates in transcription initiation by E. Coli RNA polymerase by Thederahn, T, Spassky, A, Kuwabara, M D, Sigman, D S

“…The nuclease activity of the copper complex of 5-phenyl-1,10-phenanthroline (5-phi-OP-Cu) detects conformational changes in the lac UV-5 promoter caused by E…”
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Sequence specificity of the deoxyribonuclease activity of 1,10-phenanthroline-copper ion by Yoon, Chun, Kuwabara, Michio D, Spassky, Annick, Sigman, David S

“…A statistical analysis of a data set composed of over 1600 scission events of DNA produced by the 2:1 1,10-phenanthroline-copper complex (OP-Cu) has…”
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Influence of the Local Helical Conformation on the Guanine Modifications Generated from One-Electron DNA Oxidation by Spassky, Annick, Angelov, Dimitar

“…Two major products, 2,2-diamino-4-[(2-deoxy-β-d-erythro-pentafuranosyl)amino]-5-(2H)-oxazolone and its imidazole derivative have been generated from…”
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Origin of the Heterogeneous Distribution of the Yield of Guanyl Radical in UV Laser Photolyzed DNA by Angelov, Dimitar, Beylot, Benedicte, Spassky, Annick

“…Oxidative guanine lesions were analyzed, at the nucleotide level, within DNA exposed to nanosecond ultraviolet (266 nm) laser pulses of variable intensity…”
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Temperature-dependence of UV Laser One-electron Oxidative Guanine Modifications as a Probe of Local Stacking Fluctuations and Conformational Transitions by Spassky, Annick, Angelov, Dimitar

“…By monitoring R pip/ R Fpg, i.e. the relative sensitivity to hot piperidine and to formamidopyrimidine DNA glycosylase (Fpg protein) of the guanine lesions…”
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Passage of RNA polymerase from open complex to elongation mode at the Escherichia coli lacUV5 promoter: nucleolytic hypersensitivity as a probe for complex conformational changes by Spassky, Annick

“…In transcriptionally active complexes between RNA polymerase and promoters, the center of the melted region is hyperreactive to the nucleolytic activity of the…”
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DNA-stacking interactions Determine the Sequence Specificity of the Deoxyribonuclease Activity of 1,10-Phenanthroline-copper Ion by Schaeffer, Francis, Rimsky, Sylvie, Spassky, Annick

“…Bis(1,10-phenanthroline)-copper(I) ion (OP 2Cu +) binds reversibly to B-DNA and makes single-stranded cuts by oxidative attack on the deoxyribose moiety. The…”
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Visualization of the movement of the Escherichia coli RNA polymerase along the lac UV5 promoter during the initiation of the transcription by Spassky, A

“…Three probes have been used to detect changes in the contacts between Escherichia coli RNA polymerase and lac UV5 promoter during the formation of the open…”
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Hla, an E. coli DNA-binding protein which accumulates in stationary phase, strongly compacts DNA in vitro by Spassky, Annick, Rimsky, Sylvie, Garreau, Hervé, Buc, Henri

“…We characterize a component of the E. coli bacterial nucleoid Hla, which accumulates in stationary phase. This protein, identical with the major component of a…”
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Sequence determinants for H1 binding on Escherichia coli lac and gal promoters by Rimsky, Sylvie, Spassky, Annick

“…The H1 protein is a likely candidate for structuring DNA in the bacterial nucleoid. We have studied determinants leading to its binding to DNA (and in…”
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A Picosecond Flash-Photolysis Study of the Biphotonic Ionization of Psoralen Derivatives and Ethidium Bromide by Shaquiri, Zechir, Keskinova, Elka, Spassky, Annick, Angelov, Dimitar

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Formation of cyclobutane thymine dimers from UVA photosensitization of pyridopsoralen monoadducted DNA by Guillo, L A, Beylot, B, Vigny, P, Spassky, A

“…The present report provides evidence that thymine dimerization can be UVA photosensitized at a tetranucleotide, 5'-TATT-3', by a 7-methyl-pyrido(3,4-c)psoralen…”
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Histone-like protein H1 (H-NS), DNA supercoiling, and gene expression in bacteria by Hulton, C S, Seirafi, A, Hinton, J C, Sidebotham, J M, Waddell, L, Pavitt, G D, Owen-Hughes, T, Spassky, A, Buc, H, Higgins, C F

“…Changes in DNA supercoiling in response to environmental signals such as osmolarity, temperature, or anaerobicity appear to play an underlying role in the…”
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Mapping of Single-Stranded Regions in Duplex DNA at the Sequence Level: Single-Strand-Specific Cytosine Methylation in RNA Polymerase-Promoter Complexes by Kirkegaard, Karla, Buc, Henri, Spassky, Annick, Wang, James C.

“…A method based on the differential rate of cytosine methylation in single- and double-stranded nucleic acids by dimethyl sulfate [Peattie, D. A. & Gilbert, W…”
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RNA polymerase makes important contacts upstream from base pair -49 at the Escherichia coli galactose operon P1 promoter by Busby, S, Spassky, A, Chan, B

“…A G:C to T:A transversion at bp position -19 in the gal operon promoter region relieves the dependence of galP1 promoter activity on the cAMP-CRP complex…”
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Selective DNA thymine dimerization during UVA irradiation in the presence of a saturated pyridopsoralen by Guillo, L A, Blais, J, Vigny, P, Spassky, A

“…It has been recently shown that UVA (320-400 nm) irradiation of DNA in the presence of pyridopsoralens induces the formation of thymine cyclobutane dimers in…”
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Studies with the Escherichia coli galactose operon regulatory region carrying a point mutation that simultaneously inactivates the two overlapping promoters Interactions with RNA polymerase and the cyclic AMP receptor protein by Ponnambalam, Sreenivasan, Spassky, Annick, Busby, Stephen

“…We report in vitro studies of the interactions between purified E. coli RNA polymerase and DNA from the regulatory region of the E. coli galactose operon which…”
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RNA polymerase mutant able to express in vivo and in vitro the lactose operon in the absence of the cAMP-CRP complex by Guidi-Rontani, C, Spassky, A

“…By genetic analysis, we have localized a new mutation, isolated from rho-crp background, responsible for a carbohydrate-positive phenotype. The mutation maps…”
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