Search Results - "Spagnoli, Roberto"

Total biosynthesis of hydrocortisone from a simple carbon source in yeast by Duport, Catherine, Testet, Eric, Pompon, Denis, Villeret, Coralie, Achstetter, Tilman, Cauet, Gilles, Spagnoli, Roberto, Szczebara, Florence Ménard, Costaglioli, Patricia, Winter, Jacques, Degryse, Eric, Groisillier, Agnès, Masurel, Amélie, Balbuena, David, Bourot, Stéphane, Blanchard, Sophie, Dumas, Bruno, Chandelier, Cathy

“…We report on the production of hydrocortisone, the major adrenal glucocorticoid of mammals and an important intermediate of steroidal drug synthesis, from a…”
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Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast by Duport, C. (CNRS, Gif-sur-Yvette, France.), Spagnoli, R, Degryse, E, Pompon, D

“…The first two steps of the steroidogenic pathway were reproduced in Saccharomyces cerevisiae. Engineering of sterol biosynthesis by disruption of the delta…”
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Cloning by Metabolic Interference in Yeast and Enzymatic Characterization of Arabidopsis thaliana Sterol Δ7-Reductase by Lecain, Eric, Chenivesse, Xavier, Spagnoli, Roberto, Pompon, Denis

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Sterol uptake in Saccharomyces cerevisiae heme auxotrophic mutants is affected by ergosterol and oleate but not by palmitoleate or by sterol esterification by Ness, F. (Transgene S.A., Strasbourg.), Achstetter, T, Duport, C, Karst, F, Spagnoll, R, Degryse, E

“…The relationship between sterol uptake and heme competence in two yeast strains impaired in heme synthesis, namely, G204 and H12-6A, was analyzed. To evaluate…”
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SUT1 is a putative Zn[II]2Cys6‐transcription factor whose upregulation enhances both sterol uptake and synthesis in aerobically growing Saccharomyces cerevisiae cells by Ness, Frédérique, Bourot, Stéphane, Régnacq, Matthieu, Spagnoli, Roberto, Bergès, Thierry, Karst, Francis

“…Budding yeast Saccharomyces cerevisiae is a facultative anaerobe whose growth upon oxygen starvation depends on its capacity to import exogenously supplied…”
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Pregnenolone esterification in Saccharomyces cerevisiae by Cauet, Gilles, Degryse, Eric, Ledoux, Catherine, Spagnoli, Roberto, Achstetter, Tilman

“…While studying the effect of steroids on the growth of the yeast Saccharomyces cerevisiae, we found that pregnenolone was converted into the acetate ester…”
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Erythronolide A glycosidation to erythromycin A by a blocked mutant of Streptomyces erythraeus by Spagnoli, R, Toscano, L

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Biological transformation of kanamycin A to amikacin (BBK-8) by Cappelletti, L M, Spagnoli, R

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Biological conversion of erythronolide B, an intermediate of erythromycin biogenesis, into new "hybrid" macrolide antibiotics by Spagnoli, R, Cappelletti, L, Toscano, L

“…Transformation of erythronolide B to new antibiotics was attempted by feeding this compound during the fermentation of Streptomyces antibioticus ATCC31771, a…”
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New fluorinated erythromycins obtained by mutasynthesis by Toscano, L, Fioriello, G, Spagnoli, R, Cappelletti, L, Zanuso, G

“…Following the previously described semisynthetic preparation of new aglycones (8S)-8-fluoroerythronolide A (I), (8S)-8-fluoroerythronolide B (II) and the…”
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New semisynthetic fluorinated "hybrid" macrolides by Toscano, L, Fioriello, G, Spagnoli, R, Cappelletti, L

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Soluble Fc gamma receptor type III (Fc gamma RIII, CD16) triggers cell activation through interaction with complement receptors by Galon, J, Gauchat, J-F, Mazieres, N, Spagnoli, R, Storkus, W, Loetze, M, Bonnefoy, J-Y, Fridman, W-H, Sautes, C

“…The type III-B Fc gamma receptor (Fc gamma RIII-B) is a glycosyl-phosphatidylinositol-linked receptor found on human neutrophils. A soluble form of Fc gamma…”
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Pregnenolone esterification in Saccharomyces cerevisiae a potential detoxification mechanism by Cauet, G, Degryse, E, Ledoux, C, Spagnoli, R, Achstetter, T

“…While studying the effect of steroids on the growth of the yeast Saccharomyces cerevisiae, we found that pregnenolone was converted into the acetate ester…”
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Cloning by Metabolic Interference in Yeast and Enzymatic Characterization of Arabidopsis thaliana Sterol 7-Reductase by Eric Lecain, Xavier Chenivesse, Roberto Spagnoli, Denis Pompon

“…Reduction of the Δ7 double bond of sterols, a key biosynthetic step in higher eukaryotes, is lacking in lower eukaryotes like the yeast Saccharomyces…”
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Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast by Duport, Catherine, Schoepp, Barbara, Chatelain, Elise, Spagnoli, Roberto, Dumas, Bruno, Pompon, Denis

“…Engineered yeast cells efficiently convert ergosta‐5‐eneol to pregnenolone and progesterone provided that endogenous pregnenolone acetylase activity is…”
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Biosynthesis of hydrocortisone in yeast by Dumas, Bruno, Spagnoli, Roberto

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Soluble CD16 binds peripheral blood mononuclear cells and inhibits pokeweed-mitogen-induced responses by TEILLAUD, C, GALON, J, ZILBER, M.-T, MAZIERES, N, SPAGNOLI, R, KURRLE, R, FRIDMAN, W. H, SAUTES, C

“…Human neutrophils express two types of low affinity receptors for IgG, Fc gamma RII or CD32 and Fc gamma RIIIB or CD16. Human serum contains soluble CD16…”
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11β‐Hydroxylase Activity in Recombinant Yeast Mitochondria: In vivo Conversion of 11‐Deoxycortisol to Hydrocortisone by Dumas, Bruno, Cauet, Gilles, Lacour, Thierry, Degryse, Eric, Laruelle, Laurence, Ledoux, Catherine, Spagnoli, Roberto, Achstetter, Tilman

“…In mammals, the final 11β‐hydroxylation step of the hydrocortisone biosynthesis pathway is performed by a mitochondrial enzyme, namely cytochrome P ‐450 11β ,…”
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Recombinant soluble Fc gamma receptors: production, purification and biological activities by Sautès, C, Galinha, A, Bouchard, C, Mazières, N, Spagnoli, R, Fridman, W H

“…Soluble forms of low affinity receptors for the Fc portion of IgG circulate in body fluids and regulate immune functions. We describe the transfection,…”
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SUT1 is a putative Zn[II]2Cys6-transcription factor whose upregulation enhances both sterol uptake and synthesis in aerobically growing Saccharomyces cerevisiae cells by Ness, F, Bourot, S, Régnacq, M, Spagnoli, R, Bergès, T, Karst, F

“…Budding yeast Saccharomyces cerevisiae is a facultative anaerobe whose growth upon oxygen starvation depends on its capacity to import exogenously supplied…”
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