Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)

Global proteomic analysis of the follicular fluid from brown brocket deer (Mazama gouazoubira; Fisher, 1814)

Understanding the protein composition of the follicular fluid from brown brocket deer would aid the development of a medium for in vitro embryo production, which would in turn contribute toward programs for species preservation. Proteomic shotgun is a sensitive tool for analysis of complex protein m...

Full description

Saved in:
Bibliographic Details
Published in:European journal of wildlife research Vol. 68; no. 2
Main Authors: Souza, Thais T. S., van Tilburg, Maurício F., Bezerra, Maria J. B., Rola, Luciana D., Pereira, Leda M. C., Duarte, José M. B., Chaves, Maiana S., Melo, Luciana M., Moura, Arlindo A. A. N., Freitas, Vicente J. F.
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-04-2022
Springer Nature B.V
Subjects:
Biological activity
Biomedical and Life Sciences
Catalytic activity
Coagulation
Ecology
Embryos
Extracellular matrix
Fish & Wildlife Biology & Management
Follicles
Follicular fluid
Laparoscopy
Life Sciences
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Original Article
Protein composition
Proteins
Proteomes
Proteomics
Quantitative analysis
Shotguns
Zoology
Cervid
Follicle
Shotgun
Protein
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Understanding the protein composition of the follicular fluid from brown brocket deer would aid the development of a medium for in vitro embryo production, which would in turn contribute toward programs for species preservation. Proteomic shotgun is a sensitive tool for analysis of complex protein mixtures. Thus, this study aimed to use shotgun technique to investigate the proteome of fluid from small/medium and large follicles from brocket brown deer. The fluid was obtained by laparoscopy-guided follicular puncture from five females. Quantitative proteomic analysis was performed by multidimensional liquid chromatography coupled with mass spectrometry. A total of 226 proteins were identified in the follicular fluid, 53 and 60 of which were found only in small/medium and large follicles, respectively. One hundred and thirteen proteins were common to both groups of follicles. Quantitative analysis showed no significant differences ( P  > 0.05) in protein abundances in the follicular fluid from small/medium and large follicles. The proteins identified were classified by gene ontology terms, in silico interaction and assigned to 12 pathways indicated that proteins are involved in protein binding, catalytic activity, regulation of biological processes, extracellular matrix organization, and complement and coagulation cascades. In conclusion, these data add knowledge on the follicular development and provide original information on the follicular environment, which can contribute in the future to formulation of culture medium to use in embryo technology in brown brocket deer.
ISSN:1612-4642
1439-0574
DOI:10.1007/s10344-022-01563-0