Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix

Canine mesenchymal cells (MSCs) derived from Wharton's jelly were co-cultured, then supplemented or not supplemented with platelet rich plasma (PRP) and demineralized bone matrix (DBM) to verify osteogenic differentiation. Osteoblastic differentiation followed by mineralized bone matrix product...

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Bibliographic Details
Published in:	Journal of veterinary science (Suwŏn-si, Korea) Vol. 16; no. 3; pp. 381 - 384
Main Authors:	Souza, Talita F B, Sakamoto, Silmara S, Ferreira, Gabriel T N M, Gameiro, Roberto, Marinho, Marcia, de Andrade, Alexandre L, Cardoso, Tereza C
Format:	Journal Article
Language:	English
Published:	Korea (South) 대한수의학회 2015 The Korean Society of Veterinary Science
Subjects:	Animals Bone Demineralization Technique - veterinary Bone Matrix - metabolism Cell Differentiation Cells, Cultured Coculture Techniques - veterinary Dogs Mesenchymal Stem Cells - metabolism Osteogenesis Platelet-Rich Plasma - metabolism Short Communication Umbilical Cord - metabolism 수의학 bone umbilical cord platelet-rich plasma mesenchymal stem cells
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Summary:	Canine mesenchymal cells (MSCs) derived from Wharton's jelly were co-cultured, then supplemented or not supplemented with platelet rich plasma (PRP) and demineralized bone matrix (DBM) to verify osteogenic differentiation. Osteoblastic differentiation followed by mineralized bone matrix production was found to be significantly higher (p < 0.05) when MSCs were associated with PRP/DBM in culture after 14-21-days of induction. Osteopontin and osteocalcin gene expression were significantly superior (p < 0.05) under the same culture conditions after 21 days of observation. In conclusion, addition of PRP to DBM co-cultured with MSCs successfully induced osteogenesis in vitro.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 G704-001401.2015.16.3.007 http://www.vetsci.org/journal/download_pdf.php?spage=381&volume=16&number=3
ISSN:	1229-845X 1976-555X
DOI:	10.4142/jvs.2015.16.3.381