Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts

The efficiency of the chemiluminescence luminol method and colorimetric DPPH and ABTS methods in evaluating the antiradical capacity of pure compounds and plant extracts with antioxidant potential is compared. In case of pure compounds, the values of parameter 'n' (number of radicals quenc...

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Bibliographic Details
Published in:	Química Nova Vol. 37; no. 3; pp. 497 - 503
Main Authors:	Oliveira, Sandro de, Souza, Glalci Alves de, Eckert, Camila Rodrigues, Silva, Thuany Alves, Sobral, Edmar Silva, Fávero, Oriana Aparecida, Ferreira, Marcelo José Pena, Romoff, Paulete, Baader, Wilhelm Josef
Format:	Journal Article
Language:	English
Published:	Sociedade Brasileira de Química 01-01-2014
Subjects:	antioxidant assays luminol chemiluminescence Trolox® percentage
Online Access:	Get full text
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Summary:	The efficiency of the chemiluminescence luminol method and colorimetric DPPH and ABTS methods in evaluating the antiradical capacity of pure compounds and plant extracts with antioxidant potential is compared. In case of pure compounds, the values of parameter 'n' (number of radicals quenched per molecule of antiradical) for ascorbic acid, p-hydroquinone, catechol, quercetin, and rutin are similar when measured by colorimetric assays; however, considerably lower values of n are obtained with the luminol assay. The antiradical activity of extracts from male and female individuals of Baccharis burchelli and Baccharis crispa were determined by the luminol assay and expressed using the new Trolox® percentage (%Trolox®) parameter.
ISSN:	0100-4042 1678-7064
DOI:	10.5935/0100-4042.20140076