Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil

Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil

Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African contine...

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Published in:Infection, genetics and evolution Vol. 85; p. 104550
Main Authors: Rabahi, Marcelo Fouad, Conceição, Emilyn Costa, de Paiva, Luísa Oliveira, Souto, Marcos Vinícius Muniz Lemes, Sisco, Maria Carolina, de Waard, Jacobus, de Souza Caldas, Paulo Cesar, Fandinho, Fatima, Ramos, Jesus Pais, de Carvalho, Luciana Distasio, Campos, Carlos Eduardo Dias, Lima, Karla Valéria Batista, da Silva, Sandro Patroca, Sharma, Abhinav, Robledo, Jaime, Paez, Uriel Alonso Hurtado, Duarte, Rafael Silva, da Silva, Marlei Gomes, Gomes, Lia Lima, Vasconcellos, Sidra Ezidio Gonçalves, Uwezeye, Cecile, de Jong, Bouke C., Junqueira-Kipnis, Ana Paula, Suffys, Philip Noel
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-11-2020
Subjects:
Brazil
Case report
Genome
Molecular epidemiology
Mycobacterium tuberculosis var. africanum
Tuberculosis
ATS
WGS
DST
MTBC
CTAB
RPM
TB
STR
Case report
RD
Tuberculosis
MAF
SNP
Mycobacterium tuberculosis var. africanum
Molecular epidemiology
Brazil
MTB
EMB
Genome
AFB
LJ
SIT
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Summary:Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case. •First case of tuberculosis caused by MAF in Brazil.•Extensive genotyping identification of the isolate.•Discussion of lack of detection of MAF due to incorrect procedures.•Close phylogenomics relationship with MAF isolated from The Gambia.
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ISSN:1567-1348
1567-7257
DOI:10.1016/j.meegid.2020.104550