Search Results - "Southworth, M W"

Control of protein splicing by intein fragment reassembly by Southworth, Maurice W., Adam, Eric, Panne, Daniel, Byer, Robyn, Kautz, Roger, Perler, Francine B.

“…Inteins are protein splicing elements that mediate their excision from precursor proteins and the joining of the flanking protein sequences (exteins). In this…”
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Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein by Southworth, M W, Amaya, K, Evans, T C, Xu, M Q, Perler, F B

“…The Mycobacterium xenopi gyrase A mini-intein has been engineered to yield a controllable N-terminal or C-terminal, single-splice-junction autocleavage…”
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An alternative protein splicing mechanism for inteins lacking an N-terminal nucleophile by Southworth, Maurice W., Benner, Jack, Perler, Francine B.

“…Variations in the intein‐mediated protein splicing mechanism are becoming more apparent as polymorphisms in conserved catalytic residues are identified. The…”
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Cloning of Thermostable DNA Polymerases from Hyperthermophilic Marine Archaea with Emphasis on Thermococcus sp. 9 degrees N-7 and Mutations Affecting 3′-5′ Exonuclease Activity by Southworth, M. W., Kong, H., Kucera, R. B., Ware, J., Jannasch, H. W., Perler, F. B.

“…Five extremely thermophilic Archaea from hydrothermal vents were isolated, and their DNA polymerases were cloned and expressed in Escherichia coli. Protein…”
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Rescue of protein splicing activity from a Magnetospirillum magnetotacticum intein-like element by Southworth, M W, Yin, J, Perler, F B

“…The self-catalytic protein splicing mechanism is mediated by the intein plus the first amino acid following the intein C-terminus (termed the +1 residue)…”
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In vitro protein splicing of purified precursor and the identification of a branched intermediate by Xu, M Q, Southworth, M W, Mersha, F B, Hornstra, L J, Perler, F B

“…Protein splicing is a posttranslational processing event in which an internal polypeptide is excised from a protein precursor and the terminal polypeptides are…”
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Identification and Purification of a Bacterial Ice-Nucleation Protein by Wolber, Paul K., Deininger, Caroline A., Southworth, Maurice W., Vandekerckhove, Joel, Van Montagu, Marc, Warren, Gareth J.

“…The protein product of a gene (inaZ) responsible for ice nucleation by Pseudomonas syringae S203 has been identified and purified after overexpression in…”
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Nonlinear relationship between concentration and activity of a bacterial ice nucleation protein by Southworth, M W, Wolber, P K, Warren, G J

“…The expression level of an ice nucleation gene (inaZ) was varied in Escherichia coli to observe the relationship between activity and gene product. The ice…”
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Rates of assembly and degradation of bacterial ice nuclei by Watanabe, N M, Southworth, M W, Warren, G J, Wolber, P K

“…The kinetics of ice-nucleus assembly from newly synthesized nucleation protein were observed following induction of nucleation gene expression in the…”
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Differential recognition of microfilarial chitinase, a transmission-blocking vaccine candidate antigen, by sera from patients with Brugian and Bancroftian filariasis by Dissanayake, S, Perler, F B, Xu, M, Southworth, M W, Yee, C K, Wang, S, Dreyer, G, Watawana, L, Kurniawan, L, Fuhrman, J A

“…We examined the reactivity of human sera with recombinant microfilarial chitinase and with the antigenic determinant on the native parasite molecule identified…”
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Expression of an Onchocerca volvulus Ov33 homolog in Dirofilaria immitis: potential in immunodiagnosis of heartworm infection by Santiago Mejia, J, Nkenfou, C, Southworth, M W, Perler, F B, Carlow, C K

“…In this study, the expression of an Onchocerca volvulus Ov33 homolog was demonstrated in Dirofilaria immitis. Rabbit antiserum raised against a recombinant…”
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Protein Splicing of the Deinococcus radiodurans Strain R1 Snf2 Intein by Southworth, Maurice W, Perler, Francine B

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Bacteriophage-Based Genetic System for Selection of Nonsplicing Inteins by CANN, Isaac K. O, AMAYA, Kensey R, SOUTHWORTH, Maurice W, PERLER, Francine B

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Influence of recF on spontaneous mutation in Escherichia coli by Southworth, M W, Bridges, B A

“…A recF mutation increased the spontaneous mutation rate for mutations to histidine and tryptophan independence, and to streptomycin resistance but not…”
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Gene cloning and production of active recombinant Brugia malayi microfilarial chitinase by Southworth, Maurice W., Fuhrman, Juliet A., Robbins, Phillips W., Beauregard, Kathryn, Perler, Francine B.

“…Canlas and coworkers [Canlas et al. (1984) Am. J. Trop. Med. Hyg. 33, 420–424] isolated a monoclonal antibody (MF1) which, upon passive transfer, led to the…”
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Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9degreesN-7 and mutations affecting 3'-5' exonuclease activity by Southworth, M W, Kong, H, Kucera, R B, Ware, J

“…Five extremely thermophilic Archaea from hydrothermal vents were isolated, and their DNA polymerases were cloned and expressed in Escherichia coli. Southworth…”
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Cloning of thermostable polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degree N-7 and mutations affecting 3'-5' exonuclease activity by Southworth, M W, Kong, H, Kucera, R B, Ware, J, Jannasch, H W, Perler, F B

“…Five extremely thermophilic Archaea from hydrothermal vents were isolated, and their DNA polymerases were cloned and expressed in Escherichia coli. Protein…”
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The effect of uv irradiation on mutagenesis in escherichia coli by Southworth, M W

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Mutagenic repair in Escherichia coli. VIII. Effect of gyrB mutations on ultraviolet light mutagenesis by Bridges, B A, Southworth, M W, Orr, E

“…Introduction into Escherichia coli WP2 bacteria of a mutation in the gyrB locus previously shown to reduce the degree of chromosomal superhelicity caused a…”
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