Evaluation of Different Heating Systems for the Hydrolysis of Residual Frying Oil Catalyzed by Free and Immobilized Lipase

Abstract Large quantities of residual cooking oil are being increasingly generated in various sectors, as it is often improperly disposed of and causes serious environmental problems. A commonly used alternative is the use of lipases in enzymatic hydrolysis, since it is rather attractive to several...

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Published in:	Brazilian Archives of Biology and Technology Vol. 67
Main Authors:	Correia, Thais Bazilli de Almeida, Sousa, Grazielle Pereira Reis de, Reis, Willian de Souza Matias, Theodoro, Janaina Martins, Hidalgo, Vitor Bertolassi, Santos, Jorge Henrique Vieira, Rós, Patrícia Caroline Molgero Da, Pereira, Ernandes Benedito
Format:	Journal Article
Language:	English
Published:	Instituto de Tecnologia do Paraná (Tecpar) 2024
Subjects:	Burkholderia cepacia lipase 2 Hydrolysis 5 Immobilization 4 Porcine pancreas lipase 3 Residual cooking oil 1
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Summary:	Abstract Large quantities of residual cooking oil are being increasingly generated in various sectors, as it is often improperly disposed of and causes serious environmental problems. A commonly used alternative is the use of lipases in enzymatic hydrolysis, since it is rather attractive to several sectors, in addition to allowing the use of products generated thereof, such as: free fatty acids, diacylglycerols, monoacylglycerols and free glycerol, moreover, it is capable of operating in mild temperature and pressure conditions. Therefore, the present study aimed to evaluate alternatives for the hydrolysis of residual frying oil by evaluating the efficiency of different types of supports and heating systems using free and immobilized forms of lipase from Burkholderia cepacia (BCL) and Porcine pancreas (PPL) as biocatalyst. Polyhydroxybutyrate (PHB), niobium oxide (Nb2O5) and chitosan (CHIT) were evaluated as support for immobilizing BCL and PPL lipases, and superior results were found regarding the hydrolytic activity of immobilization derivatives using PHB and Nb2O5. Among the heating systems under evaluation, the highest percentage of residual oil hydrolysis was found using the ultrasound system for both free and immobilized lipases, reaching 57.91% hydrolysis for lipases immobilized on Nb2O5 and 61.11% hydrolysis for the derivative immobilized on PHB. The operational stability of both biocatalysts was evaluated using similar half-life time values for both. Thus, it was observed that the ultrasound system was efficient in improving lipase performance in the hydrolysis of free fatty acids, once this unconventional heating system is quite promising for accelerating such enzymatic reactions.
ISSN:	1516-8913 1678-4324
DOI:	10.1590/1678-4324-2024220625