Effect of incubation temperature on nutrient transporters and small intestine morphology of broiler chickens

Effect of incubation temperature on nutrient transporters and small intestine morphology of broiler chickens

This study evaluated the effects of elevated incubation temperature on posthatch nutrient transporter gene expression, integrity of the intestinal epithelium, organ development, and performance in Ross 308 broiler chickens. Ross x Ross 308 fertile eggs (n = 900) were incubated at different eggshell...

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Bibliographic Details
Published in:Poultry science Vol. 90; no. 1; pp. 118 - 125
Main Authors: Barri, A, Honaker, C.F, Sottosanti, J.R, Hulet, R.M, McElroy, A.P
Format: Journal Article
Language:English
Published: Oxford, UK Poultry Science Association 2011
Oxford University Press
Subjects:
animal reproduction
Animals
broiler chickens
Chick Embryo - physiology
Chickens - growth & development
chicks
gene expression
hatching
incubation
incubators (equipment)
Intestine, Small - anatomy & histology
Intestine, Small - growth & development
messenger RNA
morphogenesis
nutrient transport
ova
physiological response
postnatal development
Ross (chicken breed)
small intestine
Temperature
temperature profiles
Time Factors
tissue weight
transporters
nutrient transporter
morphology
incubation
broiler
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Summary:This study evaluated the effects of elevated incubation temperature on posthatch nutrient transporter gene expression, integrity of the intestinal epithelium, organ development, and performance in Ross 308 broiler chickens. Ross x Ross 308 fertile eggs (n = 900) were incubated at different eggshell temperatures during development. From embryonic day (ED) 1 to ED12, all eggs were incubated at 37.1°C, whereas from ED13 to ED21, the eggs were divided into 2 groups for incubation at 37.4°C (S) or 39.6°C (H). Performance characteristics were measured at day of hatch (DOH) and d 7, 14, 21, 30, and 42. Small intestine and residual yolk sacs were collected at DOH and d 2, 4, 6, and 10 and weighed individually. Intestinal samples from the duodenum, jejunum, and ileum were evaluated for mucosal morphology and relative nutrient transporter gene expression. No significant differences were found in performance or organ weights. The intestinal morphology results showed a temperature x age interaction in duodenum villus height (P = 0.02) and crypt depth (P = 0.05) and in ileum villus height-to-crypt depth ratios (P = 0.02). There was a main effect of temperature, resulting in deeper crypts (P = 0.02) in the jejunum of chicks incubated at H compared with S. In the nutrient gene expression evaluation, peptide transporter (PepT1) showed a temperature x age interaction. On DOH and d 2, 4, and 10, PepT1 expression was similar between chicks incubated at S and H. However, on d 6, chicks incubated at S had significantly higher expression of PepT1 than those incubated at H. This study presents the effects of elevated incubation temperature on small intestine morphology and relative expression of nutrient transporter mRNA in high-yield broiler chicks, which can be important for the availability of nutrients and distribution of energy.
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content type line 23
ISSN:0032-5791
1525-3171
DOI:10.3382/ps.2010-00908