Identification of Streptococcus sanguinis genes producing biofilm from gingivitis

Streptococcus sanguinis is a teeth commensal frontier colonizer and among the most common species in the oral biofilm. Dental plaque, caries, and gingivitis/periodontitis are caused by dysbiosis of oral flora. A biofilm assay was developed to investigate biofilm formation in S. sanguinis using the m...

Full description

Saved in:

Bibliographic Details
Published in:	Cellular and Molecular Biology Vol. 68; no. 8; pp. 34 - 40
Main Authors:	Hassan, Pakhshan Abdullah, Hameed Saeed, Chiman, Rashid, Sirwan Ahmed, Sorchee, Sawsan Mohammed, Shareef, Suhayla Hamad
Format:	Journal Article
Language:	English
Published:	France 31-08-2022
Subjects:	Biofilms Gingivitis - genetics Humans Microbiota Streptococcus sanguis - genetics
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Streptococcus sanguinis is a teeth commensal frontier colonizer and among the most common species in the oral biofilm. Dental plaque, caries, and gingivitis/periodontitis are caused by dysbiosis of oral flora. A biofilm assay was developed to investigate biofilm formation in S. sanguinis using the microtiter plate, tube, and Congo red agar methods in order to identify causing bacteria and determine responsible genes. Three genes, including pur B, thr B, and pyre E, were suspected of playing a role in forming in vivo biofilms in S. sanguinis. The present study shows these genes to be responsible for increased biofilm formation in gingivitis patients.
ISSN:	0145-5680 1165-158X
DOI:	10.14715/cmb/2022.68.8.6