Analysis of polyphenols in brewer’s spent grain and its comparison with corn silage and cereal brans commonly used for animal nutrition

Analysis of polyphenols in brewer’s spent grain and its comparison with corn silage and cereal brans commonly used for animal nutrition

•Aqueous-alkaline extraction showed the highest recovery of polyphenol monomers.•Microwave was the best extraction procedure for brans.•Maceration was better than microwave for corn silage brewer’s spent grain.•The highest antioxidant activity was found when aqueous alkaline condition is used.•Brewe...

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Bibliographic Details
Published in:Food chemistry Vol. 239; pp. 385 - 401
Main Authors: Stefanello, Flávia Santi, dos Santos, Clarissa Obem, Bochi, Vivian Caetano, Fruet, Ana Paula Burin, Soquetta, Marcela Bromenberg, Dörr, Andréa Cristina, Nörnberg, José Laerte
Format: Journal Article
Language:English
Published: England Elsevier Ltd 15-01-2018
Subjects:
Agricultural and agro-industrial waste
Alkaline hydrolysis
Animals
Antioxidant capacity
Bioactive compounds
Edible Grain
HPLC-DAD
Plant Structures
Polyphenols
Silage
Zea mays
Alkaline hydrolysis
Antioxidant capacity
Agricultural and agro-industrial waste
HPLC-DAD
Bioactive compounds
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Summary:•Aqueous-alkaline extraction showed the highest recovery of polyphenol monomers.•Microwave was the best extraction procedure for brans.•Maceration was better than microwave for corn silage brewer’s spent grain.•The highest antioxidant activity was found when aqueous alkaline condition is used.•Brewer’s spent grain has higher content of polyphenols than silage and brans. Brewer’s spent grain (BSG) could be tested as an alternative source of polyphenols in animal nutrition. Proper extraction and analytical methods are critical for quantification. Thus, extraction for BSG, corn silage, and brans of rice, corn, and wheat were studied for the highest yield of polyphenols. A method for 18 phenolic monomers by HPLC-DAD was developed, validated, and applied to samples. An aqueous solution of NaOH (0.75% w/v) using integral samples for extraction resulted in the highest values for colorimetric measurements in all analyzed sources. Method by maceration showed the highest phenolic yield when applied in corn silage and BSG. However, for brans the best method was microwave assisted. Results from HPLC-DAD analysis clearly showed that native structures of phenolic compounds were simplified to its monomers allowing quantification and sample discrimination. BSG had the highest concentration of polyphenols and could be a promising and innovative source for animal feed studies.
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ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2017.06.130