Utilization of novel molecular multiplex methods for the detection and, epidemiological surveillance of dengue virus serotypes and chikungunya virus in Burkina Faso, West Africa

Background Dengue virus (DENV) and Chikungunya virus (CHIKV) are major arboviruses that are transmitted to humans by Aedes aegypti ( A. aegypti ) and Aedes Albopictus (A. Albopictus) mosquitoes. In absence of specific antivirals and vaccine against these two viruses, prompt diagnosis of acute infect...

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Published in:	Molecular biology reports Vol. 51; no. 1; p. 906
Main Authors:	Gomgnimbou, Michel Kiréopori, Belem, Louis Robert Wendyam, Some, Kouadou, Diallo, Madina, Barro, Bakari, Kaboré, Antoinette, Hafalla, Julius Clemence R., Sangaré, Ibrahim
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Netherlands 01-12-2024 Springer Nature B.V
Subjects:	Adolescent Adult Aedes - virology Aged Aged, 80 and over Animal Anatomy Animal Biochemistry Animals Antiviral agents Biomedical and Life Sciences Burkina Faso - epidemiology Chikungunya Fever - blood Chikungunya Fever - diagnosis Chikungunya Fever - epidemiology Chikungunya Fever - virology Chikungunya virus Chikungunya virus - genetics Chikungunya virus - isolation & purification Child Child, Preschool Dengue - blood Dengue - diagnosis Dengue - epidemiology Dengue - virology Dengue fever Dengue Virus - genetics Dengue Virus - isolation & purification Disease transmission Epidemiological Monitoring Epidemiology Female Histology Humans Life Sciences Male Middle Aged Morphology Multiplex Polymerase Chain Reaction - methods Original Article Patients Polymerase chain reaction Serogroup Serotypes Serotyping Vector-borne diseases Viruses Young Adult Burkina Faso Burkina Faso Surveillance Detection Dengue virus Molecular multiplex methods Chikungunya virus
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Summary:	Background Dengue virus (DENV) and Chikungunya virus (CHIKV) are major arboviruses that are transmitted to humans by Aedes aegypti ( A. aegypti ) and Aedes Albopictus (A. Albopictus) mosquitoes. In absence of specific antivirals and vaccine against these two viruses, prompt diagnosis of acute infections and robust surveillance for outbreak identification remain crucial. Therefore, rapid, robust, high-throughput, accessible, and low-cost assays are essential for endemic countries. This study evaluated our recently developed multiplex RT-PCR and RT-qPCR assays to screen for DENV1-4 and CHIKV circulation in Burkina Faso. Methods and results This study, conducted between June to August 2023, enrolled patients with suspected arbovirus infection presenting at healthcare facilities in three Burkina Faso cities (Bobo-Dioulasso, Houndé, and Ouagadougou). Serum samples were collected and screened for DENV serotypes and CHIKV using our newly multiplex RT-PCR and RT-q PCR techniques recently developed. A total of 408 patients (age median = 33, range from 3 to 84 years) participated in this study. Of these, 13.7% (56/408) had DENV infection; DENV-1 was 32.1% (18/56) and DENV-3 was 67.9% (38/56). DENV-2, DENV-4 and CHIKV were not detected. Conclusions This study demonstrates the effectiveness of our molecular methods for DENV detection and serotyping in Burkina Faso. The affordability of our methods makes them valuable for implementing widespread routine clinical diagnostics or arbovirus surveillance in resource-limited settings.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0301-4851 1573-4978 1573-4978
DOI:	10.1007/s11033-024-09847-1