Search Results - "Soga, Keisuke"
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Rapid Screening Detection of Genetically Modified Papaya by Loop-Mediated Isothermal Amplification
Published in Biological & pharmaceutical bulletin (01-05-2023)“…A loop-mediated isothermal amplification (LAMP)-mediated screening detection method for genetically modified (GM) papaya was developed targeting the 35S…”
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Unbiased prediction of off‐target sites in genome‐edited rice using SITE‐Seq analysis on a web‐based platform
Published in Genes to cells : devoted to molecular & cellular mechanisms (01-12-2022)“…Genome‐editing using the CRISPR‐Cas9 system has the potential to substantially accelerate crop breeding. Since off‐target editing is one of problems, a…”
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Development and Interlaboratory Validation of a Novel Reproducible Qualitative Method for GM Soybeans Using Comparative Cq-Based Analysis for the Revised Non-GMO Labeling System in Japan
Published in Analytical chemistry (Washington) (04-10-2022)“…Many countries have implemented the labeling system of genetically modified organisms (GMO). In Japan, the regulatory threshold for non-GMO labeling will be…”
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Development of a Novel Detection Method Targeting an Ultrashort 25 bp Sequence Found in Agrobacterium-Mediated Transformed GM Plants
Published in Journal of agricultural and food chemistry (23-12-2020)“…Agrobacterium-mediated transformation is the most commonly used technique for plant genetic engineering. During the transformation, a T-DNA region, which is…”
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Providing appropriate information to consumers boosts the acceptability of genome-edited foods in Japan
Published in GM crops & food (31-12-2023)“…The Japanese Health Ministry recently granted permission for the market distribution of genome-edited (GE) foods, yet there remains a lack of full…”
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PERFORMANCE EVALUATION OF THE EQUIPMENT FOR MEASURING RADIOACTIVITY IN WHOLE FOODSTUFFS WITHOUT DESTRUCTIVE SAMPLE PREPARATION DEVELOPED AFTER THE FUKUSHIMA NPP ACCIDENT
Published in Radiation protection dosimetry (01-10-2019)“…Abstract Recently, several types of instruments for measuring radioactivity in whole foodstuff were developed by manufacturers, in which any sample preparation…”
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Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction
Published in Food chemistry (01-02-2020)“…•A real-time PCR detection method for GM Atlantic salmon (AquAdvantage) was developed.•Detection limit of the method developed was 12.5–25 copies of targeted…”
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Phytohemagglutinin from Phaseolus vulgaris (PHA-E) displays a novel glycan recognition mode using a common legume lectin fold
Published in Glycobiology (Oxford) (01-04-2014)“…Phytohemagglutinin from Phaseolus vulgaris (PHA-E), a legume lectin, has an unusual specificity toward biantennary galactosylated N-glycan with bisecting…”
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Rapid DNA template preparation directly from a rice sample without purification for loop-mediated isothermal amplification (LAMP) of rice genes
Published in Bioscience, biotechnology, and biochemistry (02-04-2020)“…Rapid DNA template preparation directly from a single rice (Oryza sativa) grain or rice flour of its equivalent weight was developed for loop-mediated…”
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Terminal N-acetylgalactosamine-specific leguminous lectin from Wisteria japonica as a probe for human lung squamous cell carcinoma
Published in PloS one (13-12-2013)“…Millettia japonica was recently reclassified into the genus Wisteria japonica based on chloroplast and nuclear DNA sequences. Because the seed of Wisteria…”
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Mutated Leguminous Lectin Containing a Heparin-Binding like Motif in a Carbohydrate-Binding Loop Specifically Binds to Heparin
Published in PloS one (29-12-2015)“…We previously introduced random mutations in the sugar-binding loops of a leguminous lectin and screened the resulting mutated lectins for novel specificities…”
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Development and Testing of an Individual Kernel Detection System for Genetically Modified Soybean Events in Non-identity-preserved Soybean Samples
Published in Biological & pharmaceutical bulletin (2020)“…A genetically modified (GM) soybean kernel detection system using combination of DNA preparation from individual soybean kernels and event-specific real-time…”
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Verification Study of the Detection Method for Unauthorized Genetically Modified Papaya by Combining DNA Polymerases and Real-time PCR Instruments
Published in Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) (25-06-2024)“…In the Japanese official detection method for unauthorized genetically modified (GM) papayas, one of two types of real-time PCR reagents with DNA polymerase…”
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Laboratory Performance Study of the Japanese Official Method to Detect Genetically Modified Papaya Line PRSV-YK
Published in Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) (25-06-2024)“…Since the establishment of procedures for the safety assessment of food products that use recombinant DNA technology, the manufacture, import, and sale of…”
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大豆およびとうもろこし加工食品の遺伝子組換えDNA検査におけるDNA抽出精製方法の同等性確認試験
Published in 食品衛生学雑誌 (25-04-2024)Get full text
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Investigation of Genetically Modified Maize Imported into Japan in 2021/2022 and the Applicability of Japanese Official Methods
Published in Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) (25-12-2023)“…Given that the number of genetically modified (GM) maize events that have been announced as having undergone safety assessment procedures in Japan is…”
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Data representing applicability of developed growth hormone 1 (GH1) gene detection method for detecting Atlantic salmon (Salmo salar) at high specificity to processed salmon commodities
Published in Data in brief (01-12-2019)“…This article is referred to the research article entitled “Development of a novel method for specific detection of genetically modified Atlantic salmon,…”
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Mammalian Cell Surface Display as a Novel Method for Developing Engineered Lectins with Novel Characteristics
Published in Biomolecules (Basel, Switzerland) (20-07-2015)“…Leguminous lectins have a conserved carbohydrate recognition site comprising four loops (A-D). Here, we randomly mutated the sequence and length of loops C and…”
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遺伝子組換えパパイヤPRSV-YK系統特異的定性検査法を対象とした外部精度管理調査
Published in 食品衛生学雑誌 (25-06-2024)Get full text
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安全性未審査遺伝子組換えパパイヤ検知法にDNAポリメラーゼの種類とリアルタイムPCR機種の組み合わせが与える影響の検証
Published in 食品衛生学雑誌 (25-06-2024)“…安全性未審査遺伝子組換えパパイヤ検知法では,主に2種類のDNAポリメラーゼ(TaqMan Gene,FastGene)のいずれかが使用される.令和4年度に安全性未審査遺伝子組換えパパイヤPRSV-YK系統を対象に外部精度管理調査を実施したところ,PCR機種ABI7500とQS12KでTaqMan…”
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