Identification by methods of steady-state and kinetic spectrofluorimetry of endogenous porphyrins and flavins sensitizing the formation of reactive oxygen species in cancer cells

The question about acceptor molecules of optical radiation that determine the effects of photobiomodulation in relation to various types of cells still remains the focus of attention of researchers. This issue is most relevant for cancer cells, since, depending on the parameters of optical radiation...

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Published in:	Photochemistry and photobiology Vol. 100; no. 5; pp. 1310 - 1327
Main Authors:	Plavskii, Vitaly Yu, Sobchuk, Andrei N, Mikulich, Aliaksandr V, Dudinova, Olga N, Plavskaya, Ludmila G, Tretyakova, Antonina I, Nahorny, Raman K, Ananich, Tatsiana S, Svechko, Alexei D, Yakimchuk, Sergey V, Leusenka, Ihar A
Format:	Journal Article
Language:	English
Published:	United States Blackwell Publishing Ltd 01-09-2024
Subjects:	Cancer Cell death Cervical carcinoma Emission spectra Excitation spectra Flavin Fluorescence Optical properties Optical radiation Parameter identification Porphyrins Radiation Reactive oxygen species Sensitizing Spectrofluorimetry Tetrapyrroles cell extract fluorescence lifetime reactive oxygen species cancer cells steady‐state and kinetic spectrofluorimetry endogenous porphyrin flavin
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Summary:	The question about acceptor molecules of optical radiation that determine the effects of photobiomodulation in relation to various types of cells still remains the focus of attention of researchers. This issue is most relevant for cancer cells, since, depending on the parameters of optical radiation, light can either stimulate their growth or inhibit them and lead to death. This study shows that endogenous porphyrins, which have sensitizing properties, may play an important role in the implementation of the effects of photobiomodulation, along with flavins. For the first time, using steady-state and kinetic spectrofluorimetry, free-base porphyrins and their zinc complexes were discovered and identified in living human cervical epithelial carcinoma (HeLa) cells, as well as in their extracts. It has been shown that reliable detection of porphyrin fluorescence in cells is hampered by the intense fluorescence of flavins due to their high concentration (micromolar range) and higher (compared to tetrapyrroles) fluorescence quantum yield. Optimization of the spectral range of excitation and the use of extractants that provide multiple quenching of the flavin component while increasing the emission efficiency of tetrapyrroles makes it possible to weaken the contribution of the flavin component to the recorded fluorescence spectra.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0031-8655 1751-1097 1751-1097
DOI:	10.1111/php.13911