Renal effects induced by the lectin from Vatairea macrocarpa seeds

Lectins are glycoproteins that interact reversibly and specifically with carbohydrates. The renal effects of the galactose‐binding lectin from the seeds of Vatairea macrocarpa were investigated. Isolated kidneys from Wistar rats (240–280 g) were perfused with Krebs‐Henseleit solution containing 6% b...

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Bibliographic Details
Published in:	Journal of pharmacy and pharmacology Vol. 57; no. 10; pp. 1329 - 1333
Main Authors:	Monteiro, Ana M. O., Havt, Alexandre, Barbosa, Paulo S. F., Soares, Taís F., Evangelista, Janaína S. A. M., Alencar, Nylane M. N., Monteiro, Helena S. A., Martins, Alice M. C., de Menezes, Dalgimar B., Fonteles, Manassés C., Teixeira, Edson H., Pinto, Vicente P. T., Nascimento, Kyria S., Cavada, Benildo S.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-10-2005
Subjects:	Animals Dose-Response Relationship, Drug Fabaceae - chemistry Female Galactose - chemistry Galactose - pharmacology Galectins - chemistry Galectins - isolation & purification Galectins - pharmacology Glomerular Filtration Rate - drug effects Glucose - pharmacology In Vitro Techniques Kidney Glomerulus - blood supply Kidney Glomerulus - drug effects Kidney Tubules - drug effects Kidney Tubules - pathology Male Plant Lectins - chemistry Plant Lectins - isolation & purification Plant Lectins - pharmacology Rats Rats, Wistar Renal Circulation - drug effects Seeds - chemistry Time Factors Tromethamine - pharmacology Urination - drug effects Urodynamics - drug effects Vascular Resistance - drug effects
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Summary:	Lectins are glycoproteins that interact reversibly and specifically with carbohydrates. The renal effects of the galactose‐binding lectin from the seeds of Vatairea macrocarpa were investigated. Isolated kidneys from Wistar rats (240–280 g) were perfused with Krebs‐Henseleit solution containing 6% bovine serum albumin. The V. macrocarpa lectin (10 μg mL−1) increased the perfusion pressure, renal vascular resistance, urinary flow and glomerular filtration rate. However, V. macrocarpa lectin did not change the percentage sodium, potassium or chloride tubular transport. Pretreatment with lectin‐galactose complex significantly blocked the increase in perfusion pressure, renal vascular resistance, urinary flow and glomerular filtration rate. The control group showed a small amount of a proteinaceous material in the urinary space, although no alteration in the renal tubules was detected. The administration of galactose alone did not modify the functional parameters of the kidney. Kidneys perfused with V. macrocarpa lectin showed moderate deposits of a proteinaceous material in the tubules and urinary space. Those pre‐treated with lectin‐galactose complex had only small amount of a proteinaceous material in the urinary space. No abnormalities were seen in renal tubules. The results suggest that lectin from V. macrocarpa seeds has important effects on the carbohydrate‐binding sites of the renal system, given the reversal of renal effects with the use of that specific inhibitor.
Bibliography:	istex:410CF58428940981372FBA63F0EEC01F11DC3B9D ark:/67375/WNG-ZFQD6CPZ-4 ArticleID:JPHP1608 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-3573 2042-7158
DOI:	10.1211/jpp.57.10.0012