Anti-STAT6 CTL activity in Stat6−/− mice A cautionary tale

The generation of germline gene mutations in mice has been an invaluable tool for experimental biology. However, studying immune responses that develop in the absence of a specific protein that could alter thymic selection complicates experimental interpretations. We observed that CD8 + T cells from...

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Bibliographic Details
Published in:	JAK-STAT Vol. 2; no. 2; p. e24554
Main Authors:	Kaplan, Mark H., Cundiff, Judy K., Smith, Jill Stader, Aldrich, Carla J.
Format:	Journal Article
Language:	English
Published:	United States Taylor & Francis 01-04-2013 Landes Bioscience
Subjects:	autoreactivity chromium release cytotoxic T cell MHC class I Research Paper STAT6 cytotoxic T cell MHC class I autoreactivity STAT6 chromium release
Online Access:	Get full text
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Summary:	The generation of germline gene mutations in mice has been an invaluable tool for experimental biology. However, studying immune responses that develop in the absence of a specific protein that could alter thymic selection complicates experimental interpretations. We observed that CD8 + T cells from Stat6 −/− mice displayed "autoreactivity" to STAT6-expressing cells, associated with specific STAT6 peptides binding to MHC class I molecules. These results suggest caution in interpreting experiments where STAT6-expressing cells are transferred into Stat6 −/− mice, or where adoptive transfer of Stat6 −/− lymphocytes is performed. Our results further highlight additional considerations when studying immune responses involving cell transfer into gene-deficient mice.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2162-3988 2162-3996 2162-3996
DOI:	10.4161/jkst.24554