Search Results - "Smith, Jake L. R."
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Cryo-plasma FIB/SEM volume imaging of biological specimens
Published in eLife (21-02-2023)“…Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm)…”
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Ot2Rec: A semi-automatic, extensible, multi-software tomographic reconstruction workflow
Published in Biological imaging (Cambridge, England) (2023)“…Electron cryo-tomography is an imaging technique for probing 3D structures with at the nanometer scale. This technique has been used extensively in the…”
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The Mechanism of SARS-CoV-2 Nucleocapsid Protein Recognition by the Human 14-3-3 Proteins
Published in Journal of molecular biology (16-04-2021)“…[Display omitted] •SARS-CoV-2 nucleocapsid protein (N) binds to all seven human 14-3-3 isoforms.•This association with 14-3-3 strictly depends on…”
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Structural atlas of a human gut crassvirus
Published in Nature (London) (11-05-2023)“…CrAssphage and related viruses of the order Crassvirales (hereafter referred to as crassviruses) were originally discovered by cross-assembly of metagenomic…”
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Human 14-3-3 Proteins Site-selectively Bind the Mutational Hotspot Region of SARS-CoV-2 Nucleoprotein Modulating its Phosphoregulation
Published in Journal of molecular biology (30-01-2023)“…[Display omitted] •S197 and T205 are mutually exclusive 14-3-3-binding phosphosites in SARS-CoV-2 N.•T205 is the preferred binding site by all seven human…”
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The DNA polymerase of bacteriophage YerA41 replicates its T-modified DNA in a primer-independent manner
Published in Nucleic acids research (22-04-2022)“…Abstract Yersinia phage YerA41 is morphologically similar to jumbo bacteriophages. The isolated genomic material of YerA41 could not be digested by restriction…”
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A protocol for cryogenic volumetric imaging using serial plasma FIB/SEM
Published in Methods in cell biology (2023)“…Cryogenic volumetric imaging using serial plasma focused ion beam scanning electron microscopy (serial pFIB/SEM) is a new and exciting correlative volume…”
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