Search Results - "Smith, Hamilton O."

Enzymatic assembly of DNA molecules up to several hundred kilobases by Gibson, Daniel G, Young, Lei, Chuang, Ray-Yuan, Venter, J Craig, Hutchison, Clyde A, Smith, Hamilton O

“…We describe an isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA…”
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Design and synthesis of a minimal bacterial genome by Hutchison, Clyde A., Chuang, Ray-Yuan, Noskov, Vladimir N., Assad-Garcia, Nacyra, Deerinck, Thomas J., Ellisman, Mark H., Gill, John, Kannan, Krishna, Karas, Bogumil J., Ma, Li, Pelletier, James F., Qi, Zhi-Qing, Richter, R. Alexander, Strychalski, Elizabeth A., Sun, Lijie, Suzuki, Yo, Tsvetanova, Billyana, Wise, Kim S., Smith, Hamilton O., Glass, John I., Merryman, Chuck, Gibson, Daniel G., Venter, J. Craig

“…We used whole-genome design and complete chemical synthesis to minimize the 1079-kilobase pair synthetic genome of Mycoplasma mycoides JCVI-syn1.0. An initial…”
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Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome by Gibson, Daniel G, Glass, John I, Lartigue, Carole, Noskov, Vladimir N, Chuang, Ray-Yuan, Algire, Mikkel A, Benders, Gwynedd A, Montague, Michael G, Ma, Li, Moodie, Monzia M, Merryman, Chuck, Vashee, Sanjay, Krishnakumar, Radha, Assad-Garcia, Nacyra, Andrews-Pfannkoch, Cynthia, Denisova, Evgeniya A, Young, Lei, Qi, Zhi-Qing, Segall-Shapiro, Thomas H, Calvey, Christopher H, Parmar, Prashanth P, Hutchison, Clyde A. III, Smith, Hamilton O, Venter, J. Craig

“…We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence…”
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Chemical synthesis of the mouse mitochondrial genome by Gibson, Daniel G, Merryman, Chuck, Smith, Hamilton O, Hutchison, Clyde A, Venter, J Craig

“…We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro…”
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Designer diatom episomes delivered by bacterial conjugation by Karas, Bogumil J., Diner, Rachel E., Lefebvre, Stephane C., McQuaid, Jeff, Phillips, Alex P.R., Noddings, Chari M., Brunson, John K., Valas, Ruben E., Deerinck, Thomas J., Jablanovic, Jelena, Gillard, Jeroen T.F., Beeri, Karen, Ellisman, Mark H., Glass, John I., Hutchison III, Clyde A., Smith, Hamilton O., Venter, J. Craig, Allen, Andrew E., Dupont, Christopher L., Weyman, Philip D.

“…Eukaryotic microalgae hold great promise for the bioproduction of fuels and higher value chemicals. However, compared with model genetic organisms such as…”
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Essential metabolism for a minimal cell by Breuer, Marian, Earnest, Tyler M, Merryman, Chuck, Wise, Kim S, Sun, Lijie, Lynott, Michaela R, Hutchison, Clyde A, Smith, Hamilton O, Lapek, John D, Gonzalez, David J, de Crécy-Lagard, Valérie, Haas, Drago, Hanson, Andrew D, Labhsetwar, Piyush, Glass, John I, Luthey-Schulten, Zaida

“…JCVI-syn3A, a robust minimal cell with a 543 kbp genome and 493 genes, provides a versatile platform to study the basics of life. Using the vast amount of…”
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One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome by Gibson, Daniel G, Benders, Gwynedd A, Axelrod, Kevin C, Zaveri, Jayshree, Algire, Mikkel A, Moodie, Monzia, Montague, Michael G, Venter, J. Craig, Smith, Hamilton O, Hutchison, Clyde A. III

“…We previously reported assembly and cloning of the synthetic Mycoplasma genitalium JCVI-1.0 genome in the yeast Saccharomyces cerevisiae by recombination of…”
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Genome Transplantation in Bacteria: Changing One Species to Another by Lartigue, Carole, Glass, John I, Alperovich, Nina, Pieper, Rembert, Parmar, Prashanth P, Hutchison, Clyde A. III, Smith, Hamilton O, Venter, J. Craig

“…As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole…”
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Essential Genes of a Minimal Bacterium by Glass, John I., Assad-Garcia, Nacyra, Alperovich, Nina, Yooseph, Shibu, Lewis, Matthew R., Maruf, Mahir, Hutchison, Clyde A., Smith, Hamilton O., Venter, J. Craig

“…Mycoplasma genitalium has the smallest genome of any organism that can be grown in pure culture. It has a minimal metabolism and little genomic redundancy…”
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Environmental Genome Shotgun Sequencing of the Sargasso Sea by Venter, J. Craig, Remington, Karin, Heidelberg, John F., Halpern, Aaron L., Rusch, Doug, Eisen, Jonathan A., Wu, Dongying, Paulsen, Ian, Nelson, Karen E., Nelson, William, Fouts, Derrick E., Levy, Samuel, Knap, Anthony H., Lomas, Michael W., Nealson, Ken, White, Owen, Peterson, Jeremy, Hoffman, Jeff, Parsons, Rachel, Baden-Tillson, Holly, Pfannkoch, Cynthia, Rogers, Yu-Hui, Smith, Hamilton O.

“…We have applied "whole-genome shotgun sequencing" to microbial populations collected en masse on tangential flow and impact filters from seawater samples…”
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Direct transfer of whole genomes from bacteria to yeast by Karas, Bogumil J, Jablanovic, Jelena, Sun, Lijie, Ma, Li, Goldgof, Gregory M, Stam, Jason, Ramon, Adi, Manary, Micah J, Winzeler, Elizabeth A, Venter, J Craig, Weyman, Philip D, Gibson, Daniel G, Glass, John I, Hutchison, Clyde A, Smith, Hamilton O, Suzuki, Yo

“…A direct transfer of bacterial genomic DNA to yeast enables genome engineering in genetically intractable bacteria. Transfer of genomes into yeast facilitates…”
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Heterologous expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] hydrogenases in Synechococcus elongatus by Weyman, Philip D, Vargas, Walter A, Tong, Yingkai, Yu, Jianping, Maness, Pin-Ching, Smith, Hamilton O, Xu, Qing

“…Oxygen-tolerant [NiFe] hydrogenases may be used in future photobiological hydrogen production systems once the enzymes can be heterologously expressed in host…”
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Gross Chromosomal Rearrangements in Kluyveromyces marxianus Revealed by Illumina and Oxford Nanopore Sequencing by Ding, Lin, Macdonald, Harrison D, Smith, Hamilton O, Hutchison Iii, Clyde A, Merryman, Chuck, Michael, Todd P, Abramson, Bradley W, Kannan, Krishna, Liang, Joe, Gill, John, Gibson, Daniel G, Glass, John I

“…( ) is an increasingly popular industrially relevant yeast. It is known to possess a highly efficient non-homologous end joining (NHEJ) pathway that promotes…”
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Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling by Suzuki, Yo, Assad-Garcia, Nacyra, Kostylev, Maxim, Noskov, Vladimir N, Wise, Kim S, Karas, Bogumil J, Stam, Jason, Montague, Michael G, Hanly, Timothy J, Enriquez, Nico J, Ramon, Adi, Goldgof, Gregory M, Richter, R Alexander, Vashee, Sanjay, Chuang, Ray-Yuan, Winzeler, Elizabeth A, Hutchison, 3rd, Clyde A, Gibson, Daniel G, Smith, Hamilton O, Glass, John I, Venter, J Craig

“…The availability of genetically tractable organisms with simple genomes is critical for the rapid, systems-level understanding of basic biological processes…”
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Tuning Gene Activity by Inducible and Targeted Regulation of Gene Expression in Minimal Bacterial Cells by Mariscal, Ana M, Kakizawa, Shigeyuki, Hsu, Jonathan Y, Tanaka, Kazuki, González-González, Luis, Broto, Alicia, Querol, Enrique, Lluch-Senar, Maria, Piñero-Lambea, Carlos, Sun, Lijie, Weyman, Philip D, Wise, Kim S, Merryman, Chuck, Tse, Gavin, Moore, Adam J, Hutchison, Clyde A, Smith, Hamilton O, Tomita, Masaru, Venter, J. Craig, Glass, John I, Piñol, Jaume, Suzuki, Yo

“…Functional genomics studies in minimal mycoplasma cells enable unobstructed access to some of the most fundamental processes in biology. Conventional…”
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[NiFe] Hydrogenase from Alteromonas macleodii with Unusual Stability in the Presence of Oxygen and High Temperature by Vargas, Walter A, Weyman, Philip D, Tong, Yingkai, Smith, Hamilton O, Xu, Qing

“…Hydrogenases are enzymes involved in the bioproduction of hydrogen, a clean alternative energy source whose combustion generates water as the only end product…”
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Global Transposon Mutagenesis and a Minimal Mycoplasma Genome by Hutchison, Clyde A., Peterson, Scott N., Gill, Steven R., Cline, Robin T., White, Owen, Fraser, Claire M., Smith, Hamilton O., Venter, J. Craig

“…Mycoplasma genitalium with 517 genes has the smallest gene complement of any independently replicating cell so far identified. Global transposon mutagenesis…”
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Kinetic Modeling of the Genetic Information Processes in a Minimal Cell by Thornburg, Zane R, Melo, Marcelo C R, Bianchi, David, Brier, Troy A, Crotty, Cole, Breuer, Marian, Smith, Hamilton O, Hutchison, 3rd, Clyde A, Glass, John I, Luthey-Schulten, Zaida

“…JCVI-syn3A is a minimal bacterial cell with a 543 kbp genome consisting of 493 genes. For this slow growing minimal cell with a 105 min doubling time, we…”
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Designed surface residue substitutions in [NiFe] hydrogenase that improve electron transfer characteristics by Yonemoto, Isaac T, Smith, Hamilton O, Weyman, Philip D

“…Photobiological hydrogen production is an attractive, carbon-neutral means to convert solar energy to hydrogen. We build on previous research improving the…”
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A systems biology tour de force for a near-minimal bacterium by Glass, John I, Venter, J Craig, Hutchison III, Clyde A, Smith, Hamilton O

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