Search Results - "Smith, Anja van Brabant"

Systematic evaluation of antibody-mediated siRNA delivery using an industrial platform of THIOMAB-siRNA conjugates by Cuellar, Trinna L, Barnes, Dwight, Nelson, Christopher, Tanguay, Joshua, Yu, Shang-Fan, Wen, Xiaohui, Scales, Suzie J, Gesch, Julie, Davis, David, van Brabant Smith, Anja, Leake, Devin, Vandlen, Richard, Siebel, Christian W

“…Delivery of siRNA is a key hurdle to realizing the therapeutic promise of RNAi. By targeting internalizing cell surface antigens, antibody-siRNA complexes…”
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Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity by Basila, Megan, Kelley, Melissa L, Smith, Anja van Brabant

“…Since its initial application in mammalian cells, CRISPR-Cas9 has rapidly become a preferred method for genome engineering experiments. The Cas9 nuclease is…”
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Amide linkages mimic phosphates in RNA interactions with proteins and are well tolerated in the guide strand of short interfering RNAs by Mutisya, Daniel, Hardcastle, Travis, Cheruiyot, Samwel K, Pallan, Pradeep S, Kennedy, Scott D, Egli, Martin, Kelley, Melissa L, Smith, Anja van Brabant, Rozners, Eriks

“…While the use of RNA interference (RNAi) in molecular biology and functional genomics is a well-established technology, in vivo applications of synthetic short…”
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Conjugation and Evaluation of Triazole-Linked Single Guide RNA for CRISPR-Cas9 Gene Editing by He, Kaizhang, Chou, Eldon T., Begay, Shawn, Anderson, Emily M., van Brabant Smith, Anja

“…The CRISPR‐Cas9 gene editing system requires Cas9 endonuclease and guide RNAs (either the natural dual RNA consisting of crRNA and tracrRNA or a chimeric…”
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Lactobacillus gasseri CRISPR-Cas9 characterization In Vitro reveals a flexible mode of protospacer-adjacent motif recognition by Anderson, Emily M, McClelland, Shawn, Maksimova, Elena, Strezoska, Žaklina, Basila, Megan, Briner, Alexandra E, Barrangou, Rodolphe, Smith, Anja van Brabant

“…While the CRISPR-Cas9 system from S. pyogenes is a powerful genome engineering tool, additional programmed nucleases would enable added flexibility in…”
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Optimized PCR conditions and increased shRNA fold representation improve reproducibility of pooled shRNA screens by Strezoska, Žaklina, Licon, Abel, Haimes, Josh, Spayd, Katie Jansen, Patel, Kruti M, Sullivan, Kevin, Jastrzebski, Katarzyna, Simpson, Kaylene J, Leake, Devin, van Brabant Smith, Anja, Vermeulen, Annaleen

“…RNAi screening using pooled shRNA libraries is a valuable tool for identifying genetic regulators of biological processes. However, for a successful pooled…”
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Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference by Barrangou, Rodolphe, Birmingham, Amanda, Wiemann, Stefan, Beijersbergen, Roderick L, Hornung, Veit, Smith, Anja van Brabant

“…The discovery that the machinery of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 bacterial immune system can be re-purposed to…”
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Systematic analysis of CRISPR–Cas9 mismatch tolerance reveals low levels of off-target activity by Anderson, Emily M., Haupt, Amanda, Schiel, John A., Chou, Eldon, Machado, Hidevaldo B., Strezoska, Žaklina, Lenger, Steve, McClelland, Shawn, Birmingham, Amanda, Vermeulen, Annaleen, Smith, Anja van Brabant

“…•CRISPR–Cas9 with synthetic crRNA: tracrRNA is an efficient system for gene editing.•We tested 100s of mismatched-to-target crRNAs in a high-throughput…”
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An aptamer-mediated base editing platform for simultaneous knockin and multiple gene knockout for allogeneic CAR-T cells generation by Porreca, Immacolata, Blassberg, Robert, Harbottle, Jennifer, Joubert, Bronwyn, Mielczarek, Olga, Stombaugh, Jesse, Hemphill, Kevin, Sumner, Jonathan, Pazeraitis, Deividas, Touza, Julia Liz, Francescatto, Margherita, Firth, Mike, Selmi, Tommaso, Collantes, Juan Carlos, Strezoska, Zaklina, Taylor, Benjamin, Jin, Shengkan, Wiggins, Ceri M, van Brabant Smith, Anja, Lambourne, John J

“…Gene editing technologies hold promise for enabling the next generation of adoptive cellular therapies. In conventional gene editing platforms that rely on…”
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The ORFeome Collaboration: a genome-scale human ORF-clone resource by Wiemann, Stefan, Pennacchio, Christa, Hu, Yanhui, Hunter, Preston, Harbers, Matthias, Amiet, Alexandra, Bethel, Graeme, Busse, Melanie, Carninci, Piero, Dunham, Ian, Hao, Tong, Harper, J Wade, Hayashizaki, Yoshihide, Heil, Oliver, Hennig, Steffen, Hotz-Wagenblatt, Agnes, Jang, Wonhee, Jocker, Anika, Kawai, Jun, Koenig, Christoph, Korn, Bernhard, Lambert, Cristen, LeBeau, Anita, Lu, Sun, Maurer, Johannes, Moore, Troy, Ohara, Osamu, Park, Jin, Rolfs, Andreas, Salehi-Ashtiani, Kourosh, Seiler, Catherine, Simmons, Blake, van Brabant Smith, Anja, Steel, Jason, Wagner, Lukas, Weaver, Tom, Wellenreuther, Ruth, Yang, Shuwei, Vidal, Marc, Gerhard, Daniela S, LaBaer, Joshua, Temple, Gary, Hill, David E

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A Single Amide Linkage in the Passenger Strand Suppresses Its Activity and Enhances Guide Strand Targeting of siRNAs by Hardcastle, Travis, Novosjolova, Irina, Kotikam, Venubabu, Cheruiyot, Samwel K, Mutisya, Daniel, Kennedy, Scott D, Egli, Martin, Kelley, Melissa L, Smith, Anja van Brabant, Rozners, Eriks

“…Potential in vivo applications of RNA interference (RNAi) require suppression of various off-target activities. Herein, we report that replacement of a single…”
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A Facile, Transfection-Free Approach to siRNA Delivery in In Vitro 3D Spheroid Models by Riching, Andrew S, Malloy, Allyson, Anderson, Emily M, Sheard, Jonathan, Mikkonen, Piia, van Brabant Smith, Anja, Strezoska, Zaklina, Levenga, Josien

“…Cell culture has long been essential for preclinical modeling of human development and disease. However, conventional two-dimensional (2D) cell culture fails…”
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ErCas12a CRISPR-MAD7 for Model Generation in Human Cells, Mice, and Rats by Liu, Zhenyi, Schiel, John A, Maksimova, Elena, Strezoska, Žaklina, Zhao, Guojun, Anderson, Emily M, Wu, Yumei, Warren, Joe, Bartels, Angela, van Brabant Smith, Anja, Lowe, Chris E, Forbes, Kevin P

“…MAD7 is an engineered class 2 type V-A CRISPR-Cas (Cas12a/Cpf1) system isolated from Analogous to Cas9, it is an RNA-guided nuclease with demonstrated gene…”
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The Power Decoder Simulator for the Evaluation of Pooled shRNA Screen Performance by Stombaugh, Jesse, Licon, Abel, Strezoska, Žaklina, Stahl, Joshua, Anderson, Sarah Bael, Banos, Michael, van Brabant Smith, Anja, Birmingham, Amanda, Vermeulen, Annaleen

“…RNA interference screening using pooled, short hairpin RNA (shRNA) is a powerful, high-throughput tool for determining the biological relevance of genes for a…”
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Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing by Kelley, Melissa L., Strezoska, Žaklina, He, Kaizhang, Vermeulen, Annaleen, Smith, Anja van Brabant

“…•CRISPR-Cas9 with synthetic crRNA:tracrRNA is an efficient system for gene editing.•Chemically synthesized guide RNAs allow for incorporation of chemical…”
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CRISPR-mediated transcriptional activation with synthetic guide RNA by Strezoska, Žaklina, Dickerson, Sarah Michelle, Maksimova, Elena, Chou, Eldon, Gross, Maren Mayer, Hemphill, Kevin, Hardcastle, Travis, Perkett, Matthew, Stombaugh, Jesse, Miller, Galen W., Anderson, Emily M., Vermeulen, Annaleen, Smith, Anja van Brabant

“…•First use of synthetic crRNA for transcriptional activation using several CRISPRa systems.•crRNA can be used with canonical tracrRNA for the VPR CRISPRa…”
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High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries by Strezoska, Žaklina, Perkett, Matthew R., Chou, Eldon T., Maksimova, Elena, Anderson, Emily M., McClelland, Shawn, Kelley, Melissa L., Vermeulen, Annaleen, Smith, Anja van Brabant

“…•First use of arrayed, synthetic crRNAs in a multiparametric gene knockout screen.•High-content analysis was used to identify hits in a cell cycle reporter…”
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A Novel CRISPR Interference Effector Enabling Functional Gene Characterization with Synthetic Guide RNAs by Mills, Clarence, Riching, Andrew, Keller, Ashleigh, Stombaugh, Jesse, Haupt, Amanda, Maksimova, Elena, Dickerson, Sarah M, Anderson, Emily, Hemphill, Kevin, Ebmeier, Chris, Schiel, John A, Levenga, Josien, Perkett, Matthew, Smith, Anja van Brabant, Strezoska, Zaklina

“…While CRISPR interference (CRISPRi) systems have been widely implemented in pooled lentiviral screening, there has been limited use with synthetic guide RNAs…”
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Increasing gene editing efficiencies in eukaryotic cell lines by selection of appropriate CRISPR-Cas9 reagents by Kelley, Melissa L, Strezoska, Zaklina, Machado, Hidevaldo, Anderson, Emily, Mayer, Maren, Vermeulen, Annaleen, McClelland, Shawn, van Brabant Smith, Anja

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