Search Results - "Smith, Addison K"

Parameterization of Unnatural Amino Acids with Azido and Alkynyl R‑Groups for Use in Molecular Simulations by Smith, Addison K, Wilkerson, Joshua W, Knotts, Thomas A

“…Recent new methods to functionalize proteins at specific amino acid locations use unnatural amino acids that contain azido and alkynyl groups. This capability…”
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Assessing site-specific PEGylation of TEM-1 β-lactamase with cell-free protein synthesis and coarse-grained simulation by Zhao, Emily Long, Soltani, Mehran, Smith, Addison K., Hunt, J. Porter, Knotts, Thomas A., Bundy, Bradley C.

“…PEGylation is a broadly used strategy to enhance the pharmacokinetic properties of therapeutic proteins. It is well established that the location and extent of…”
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The Effects of p‑Azidophenylalanine Incorporation on Protein Structure and Stability by Wilkerson, Joshua W, Smith, Addison K, Wilding, Kristen M, Bundy, Bradley C, Knotts, Thomas A

“…Functionalization is often needed to harness the power of proteins for beneficial use but can cause losses to stability and/or activity. State of the art…”
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Assessing the predictive capabilities of design heuristics and coarse‐grain simulation toward understanding and optimizing site‐specific covalent immobilization of β‐lactamase by Soltani, Mehran, Hunt, John Porter, Smith, Addison K., Zhao, Emily Long, Knotts, Thomas A., Bundy, Bradley C.

“…For industrial applications, covalent immobilization of enzymes provides minimum leakage, recoverability, reusability, and high stability. Yet, the suitability…”
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The Locational Impact of Site-Specific PEGylation: Streamlined Screening with Cell-Free Protein Expression and Coarse-Grain Simulation by Wilding, Kristen M, Smith, Addison K, Wilkerson, Joshua W, Bush, Derek B, Knotts, Thomas A, Bundy, Bradley C

“…Although polyethylene glycol (PEG) is commonly used to improve protein stability and therapeutic efficacy, the optimal location for attaching PEG onto proteins…”
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