Search Results - "Smit, Nico P. M"

Molecular Diagnostics of Calcineurin-Related Pathologies by MUSSON, Ruben E. A, COBBAERT, Christa M, SMIT, Nico P. M

“…The Ca(2+)-dependent protein phosphatase enzyme calcineurin (Cn) (protein phosphatase 3) is best known for its role as director of the adaptive immune…”
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The Time Has Come for Quantitative Protein Mass Spectrometry Tests That Target Unmet Clinical Needs by Smit, Nico P. M, Ruhaak, L. Renee, Romijn, Fred P. H. T. M, Pieterse, Mervin M, van der Burgt, Yuri E. M, Cobbaert, Christa M

“…Protein mass spectrometry (MS) is an enabling technology that is ideally suited for precision diagnostics. In contrast to immunoassays with indirect readouts,…”
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Development and Provisional Validation of a Multiplex LC-MRM-MS Test for Timely Kidney Injury Detection in Urine by van Duijl, Tirsa T, Ruhaak, L. Renee, Smit, Nico P. M, Pieterse, Mervin M, Romijn, Fred P. H. T. M, Dolezal, Natasja, Drijfhout, Jan Wouter, de Fijter, Johan W, Cobbaert, Christa M

“…Kidney injury is a complication frequently encountered in hospitalized patients. Early detection of kidney injury prior to loss of renal function is an unmet…”
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An LC-MS-based designated comparison method with similar performance to the Lp(a) reference measurement procedure to guide molar Lp(a) standardization by Diederiks, Nina M, Ruhaak, L Renee, Romijn, Fred P H T M, Pieterse, Mervin M, Smit, Nico P M, Cobbaert, Christa M

“…The 2022 consensus statement of the European Atherosclerosis Society (EAS) on lipoprotein(a) (Lp(a)) recognizes the role of Lp(a) as a relevant genetically…”
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Variation in Leukocyte Subset Concentrations Affects Calcineurin Activity Measurement: Implications for Pharmacodynamic Monitoring Strategies by van Rossum, Huub H, Romijn, Fred P.H.T.M, Sellar, Kathryn J, Smit, Nico P.M, van der Boog, Paul J.M, de Fijter, Johan W, van Pelt, Johannes

“…In renal transplantation patients, therapeutic drug monitoring of the calcineurin (CN) inhibitor cyclosporin A (CsA) is mandatory because of the drug's narrow…”
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Quantitative protein mass-spectrometry requires a standardized pre-analytical phase by Smit, Nico P M, Romijn, Fred P H T M, van Ham, Vanessa J J, Reijnders, Esther, Cobbaert, Christa M, Ruhaak, L Renee

“…Quantitative protein mass-spectrometry (QPMS) in blood depends on tryptic digestion of proteins and subsequent measurement of representing peptides. Whether…”
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Evaluation of Interspecimen Trypsin Digestion Efficiency Prior to Multiple Reaction Monitoring-Based Absolute Protein Quantification with Native Protein Calibrators by van den Broek, Irene, Smit, Nico P. M, Romijn, Fred P. H. T. M, van der Laarse, Arnoud, Deelder, André M, van der Burgt, Yuri E. M, Cobbaert, Christa M

“…Implementation of quantitative clinical chemistry proteomics (qCCP) requires targeted proteomics approaches, usually involving bottom-up multiple reaction…”
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MS-based proteomics: a metrological sound and robust alternative for apolipoprotein E phenotyping in a multiplexed test by Ruhaak, L Renee, Smit, Nico P M, Suchiman, H Eka D, Pieterse, Mervin M, Romijn, Fred P H T M, Beekman, Marian, Cobbaert, Christa M

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Detecting molecular forms of antithrombin by LC-MRM-MS: defining the measurands by Ruhaak, L Renee, Romijn, Fred P H T M, Smit, Nico P M, van der Laarse, Arnoud, Pieterse, Mervin M, de Maat, Moniek P M, Haas, Fred J L M, Kluft, Cornelis, Amiral, Jean, Meijer, Piet, Cobbaert, Christa M

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Increased Melanogenesis is a Risk Factor for Oxidative DNA Damage- Study on Cultured Melanocytes and Atypical Nevus Cells by Smit, Nico P. M., Van Nieuwpoort, Frans A., Marrot, Laurent, Out, Coby, Poorthuis, Ben, Van Pelt, Hans, Meunier, Jean-Roch, Pavel, Stan

“…Melanin synthesis is an oxygen‐dependent process that acts as a potential source of reactive oxygen species (ROS) inside pigment‐forming cells. The synthesis…”
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Everolimus and sirolimus antagonize tacrolimus based calcineurin inhibition via competition for FK-binding protein 12 by van Rossum, Huub H., Romijn, Fred P.H.T.M., Smit, Nico P.M., de Fijter, Johan W., van Pelt, Johannes

“…The calcineurin inhibitors cyclosporin A and tacrolimus and the inhibitors of the mTOR, sirolimus and everolimus bind immunophilins that are required for their…”
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Automated Multiplex LC-MS/MS Assay for Quantifying Serum Apolipoproteins A-I, B, C-I, C-II, C-III, and E with Qualitative Apolipoprotein E Phenotyping by van den Broek, Irene, Romijn, Fred P H T M, Nouta, Jan, van der Laarse, Arnoud, Drijfhout, Jan W, Smit, Nico P M, van der Burgt, Yuri E M, Cobbaert, Christa M

“…Direct and calculated measures of lipoprotein fractions for cardiovascular risk assessment suffer from analytical inaccuracy in certain dyslipidemic and…”
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Spectrophotometric assay for calcineurin activity in leukocytes isolated from human blood by Sellar, Kathryn J., van Rossum, Huub H., Romijn, Fred P.H.T.M., Smit, Nico P.M., de Fijter, Johan W., van Pelt, Johannes

“…The calcineurin inhibitors, cyclosporine and tacrolimus, still constitute the cornerstone of immunosuppressive regimen after organ transplantation. An…”
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Mechanism of action of 4‐substituted phenols to induce vitiligo and antimelanoma immunity by Kammeyer, Arthur, Willemsen, Karin J., Ouwerkerk, Wouter, Bakker, Walbert J., Ratsma, Danielle, Pronk, Sebas D., Smit, Nico P. M., Luiten, Rosalie M.

“…Monobenzone is a 4‐substituted phenol that can induce vitiligo and antimelanoma immunity. We investigated the influence of the chemical structure on the…”
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UVA1 radiation inhibits calcineurin through oxidative damage mediated by photosensitization by Musson, Ruben E.A., Hensbergen, Paul J., Westphal, Adrie H., Temmink, Wouter P.M., Deelder, André M., van Pelt, Johannes, Mullenders, Leon H.F., Smit, Nico P.M.

“…The protein phosphatase calcineurin has been gradually revealing itself as the central controller of our immune response, although it is involved in a wide…”
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Robust and Accurate 2-Year Performance of a Quantitative Mass Spectrometry-Based Apolipoprotein Test in a Clinical Chemistry Laboratory by Ruhaak, L Renee, Smit, Nico P M, Romijn, Fred P H T M, Pieterse, Mervin M, van der Laarse, Arnoud, van der Burgt, Yuri E M, Cobbaert, Christa M

“…Analytical method validation was performed according to the Clinical and Laboratory Standards Institute (CLSI) protocols, and the intraassay CVs were 2.3% to…”
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Quantifying Protein Measurands by Peptide Measurements: Where Do Errors Arise? by van den Broek, Irene, Romijn, Fred P.H.T.M, Smit, Nico P.M, van der Laarse, Arnoud, Drijfhout, Jan W, van der Burgt, Yuri E.M, Cobbaert, Christa M

“…Clinically actionable quantification of protein biomarkers by mass spectrometry (MS) requires analytical performance in concordance with quality specifications…”
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Quantification of serum apolipoproteins A-I and B-100 in clinical samples using an automated SISCAPA–MALDI-TOF-MS workflow by van den Broek, Irene, Nouta, Jan, Razavi, Morteza, Yip, Richard, Bladergroen, Marco R., Romijn, Fred P.H.T.M., Smit, Nico P.M., Drews, Oliver, Paape, Rainer, Suckau, Detlev, Deelder, André M., van der Burgt, Yuri E.M., Pearson, Terry W., Anderson, N. Leigh, Cobbaert, Christa M.

“…[Display omitted] •A fully-automated SISCAPA–MALDI-TOF-MS assay was developed for apoA-I and apoB-100.•The assay allowed processing and analysis of 96 samples…”
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Antifibrinolytics attenuate inflammatory gene expression after cardiac surgery by Later, Alexander F.L., MD, Sitniakowsky, Laura S., MSc, van Hilten, Joost A., PhD, Watering, Leo van de, PhD, Brand, Anneke, MD, PhD, Smit, Nico P.M., PhD, Klautz, Robert J.M., MD, PhD

“…Objectives Anti-inflammatory effects of tranexamic acid and aprotinin, used to abate perioperative blood loss, are reported and might be of substantial…”
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Quality Assurance for Multiplex Quantitative Clinical Chemistry Proteomics in Large Clinical Trials by Reijnders, Esther, Romijn, Fred P H T M, Arslan, Figen, Georges, Julien J J, Pieterse, Mervin M, Schipper, Edwin R, Didden-Buitendijk, Sonja, Martherus-Bultman, Machteld C, Smit, Nico P M, Diederiks, Nina M, Treep, Maxim M, Jukema, J Wouter, Cobbaert, Christa M, Ruhaak, L Renee

“…To evaluate the clinical performance and effectiveness of a multiplex apolipoprotein panel in the context of cardiovascular precision diagnostics, clinical…”
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