Effect of chylomicron remnants on cholesterol metabolism in cultured rabbit hepatocytes: Very low density lipoprotein and bile acid production

Effect of chylomicron remnants on cholesterol metabolism in cultured rabbit hepatocytes: Very low density lipoprotein and bile acid production

The interrelationship between very low density lipoprotein (VLDL) secretion and bile acid production was studied in primary culture of rabbit hepatocytes. Chylomicron remnants (CR) were added to the cultures to study their effect on VLDL secretion and bile acid production. After 24 hr preincubation...

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Bibliographic Details
Published in:Lipids Vol. 26; no. 10; pp. 799 - 805
Main Authors: Kosykh, Vladimir A., Novikov, Dmitri K., Trakht, Iliya N., Podrez, Eugeni A., Victorov, Alexander V., Repin, Vadim S., Smirnov, Viadimir N.
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer‐Verlag 01-10-1991
Springer
Subjects:
Acetates - metabolism
Analytical, structural and metabolic biochemistry
Animals
Apolipoprotein B-48
Apolipoproteins B - metabolism
Apolipoproteins C - metabolism
Apolipoproteins E - metabolism
Bile Acids and Salts - metabolism
Biological and medical sciences
Cells, Cultured - metabolism
Cholesterol - metabolism
Chylomicrons - chemistry
Chylomicrons - pharmacology
Fundamental and applied biological sciences. Psychology
Lipoproteins, VLDL - metabolism
Liver - metabolism
Oleic Acids - metabolism
Other biological molecules
Rabbits
Lipoprotein VLDL
Liver
Rabbit
Lipids
Lipoprotein
Lagomorpha
Metabolism
Cholesterol
Proteins
Vertebrata
Mammalia
Chylomicron
Hepatocyte
Bile acid
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Summary:The interrelationship between very low density lipoprotein (VLDL) secretion and bile acid production was studied in primary culture of rabbit hepatocytes. Chylomicron remnants (CR) were added to the cultures to study their effect on VLDL secretion and bile acid production. After 24 hr preincubation of cells with CR (10–50 μg protein/mL), intercellular neutral lipid content was increased 1.5–4‐fold in a dose‐dependent manner. Neutral lipid accumulation was accompanied by a 70–90% reduction of [14C]acetate incorporation into cholesterol, while no stimulation of [14C]oleate incorporation into cholesteryl esters was observed. Incubation of cells with CR increased secretion of free cholesterol, triacylglycerol and apoproteins B and E in VLDL. Stimulation of VLDL cholesterol secretion was accompanied by a reduction of taurocholic acid synthesis. These data demonstrate the existence of an inverse relationship between secretion of VLDL cholesterol and bile acid production under conditions of effective uptake of triacylglycerol‐rich CR by hepatocytes.
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ISSN:0024-4201
1558-9307
DOI:10.1007/BF02536161