Search Results - "Smillie, Lawrence B."

Structures of the troponin C regulatory domains in the apo and calcium-saturated states by Gagné, Stéphane M, Tsuda, Sakae, Li, Monica X, Smillie, Lawrence B, Sykes, Brian D

“…Regulation of contraction in skeletal muscle occurs through calcium binding to the protein troponin C. The solution structures of the regulatory domain of apo…”
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Single Mutation (A162H) in Human Cardiac Troponin I Corrects Acid pH Sensitivity of Ca2+-regulated Actomyosin S1 ATPase by Dargis, Roland, Pearlstone, Joyce R, Barrette-Ng, Isabelle, Edwards, Helena, Smillie, Lawrence B

“…In contrast to skeletal muscle, the efficiency of the contractile apparatus of cardiac tissue has long been known to be severely compromised by acid pH as in…”
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Determinants of relaxation rate in rabbit skinned skeletal muscle fibres by Luo, Ye, Davis, Jonathan P., Smillie, Lawrence B., Rall, Jack A.

“…The influence of Ca 2+ -activated force, the rate of dissociation of Ca 2+ from troponin C (TnC) and decreased crossbridge detachment rate on the time course…”
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Engineering Competitive Magnesium Binding into the First EF-hand of Skeletal Troponin C by Davis, Jonathan P., Rall, Jack A., Reiser, Peter J., Smillie, Lawrence B., Tikunova, Svetlana B.

“…The goal of this study was to examine the mechanism of magnesium binding to the regulatory domain of skeletal troponin C (TnC). The fluorescence of Trp29,…”
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role of tropomyosin isoforms and phosphorylation in force generation in thin-filament reconstituted bovine cardiac muscle fibres by Lu, Xiaoying, Heeley, David H, Smillie, Lawrence B, Kawai, Masataka

“…The thin filament extraction and reconstitution protocol was used to investigate the functional roles of tropomyosin (Tm) isoforms and phosphorylation in…”
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Calcium Binding to the Regulatory N-Domain of Skeletal Muscle Troponin C Occurs in a Stepwise Manner by Li, Monica X, Gagne, Stephane M, Tsuda, Sakae, Kay, Cyril M, Smillie, Lawrence B, Sykes, Brian D

“…Ca2+ binding to a recombinant regulatory N-domain (residues 1-90) of chicken troponin C (NTnC) has been investigated with the use of heteronuclear…”
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Structural details of a calcium-induced molecular switch: X-ray crystallographic analysis of the calcium-saturated N-terminal domain of troponin C at 1.75 Å resolution by Strynadka, Natalie C.J., Cherney, Maia, Sielecki, Anita R., Li, Monica X., Smillie, Lawrence B., James, Michael N.G.

“…We have solved and refined the crystal and molecular structures of the calcium-saturated N-terminal domain of troponin C (TnC) to 1.75 Å resolution. This has…”
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Quantification of the calcium‐induced secondary structural changes in the regulatory domain of troponin‐C by Gagné, Stéphane M., Tsuda, Sakae, Li, Monica X., Chandra, Murali, Smillie, Lawrence B., Sykes, Brian D.

“…The backbone resonance assignments have been completed for the apo (1H and 15N) and calcium‐loaded (1H, 15N, and 13C) regulatory N‐domain of chicken skeletal…”
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Troponin I Binds Polycystin-l and Inhibits Its Calcium-Induced Channel Activation by Li, Qiang, Liu, Yan, Shen, Patrick Y, Dai, Xiao-Qing, Wang, Shaohua, Smillie, Lawrence B, Sandford, Richard, Chen, Xing-Zhen

“…Polycystin-l (PCL) is an isoform of polycystin-2, the product of the second gene associated with autosomal dominant polycystic kidney disease, and functions as…”
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Kinetic studies of calcium and cardiac troponin I peptide binding to human cardiac troponin C using NMR spectroscopy by Li, Monica X, Saude, Erik J, Wang, Xu, Pearlstone, Joyce R, Smillie, Lawrence B, Sykes, Brian D

“…Ca2+ and human cardiac troponin I (cTnI) peptide binding to human cardiac troponin C (cTnC) have been investigated with the use of 2D [1H,15N] HSQC NMR…”
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Interactions of Structural C and Regulatory N Domains of Troponin C with Repeated Sequence Motifs in Troponin I by Pearlstone, Joyce R, Sykes, Brian D, Smillie, Lawrence B

“…The actomyosin ATPase inhibitory protein troponin I (TnI) plays a central regulatory role in skeletal and cardiac muscle contraction and relaxation through its…”
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Role of Hydration in the Closed-to-Open Transition Involved in Ca2+ Binding by Troponin C by Suarez, Marisa C, Machado, Cosme José V, Lima, Luís Maurício T. R, Smillie, Lawrence B, Pearlstone, Joyce R, Silva, Jerson L, Sorenson, Martha M, Foguel, Débora

“…Troponin C (TnC) is the Ca2+-binding subunit of the troponin complex of vertebrate skeletal muscle. It consists of two structurally homologous domains, N and…”
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Defining the Region of Troponin-I that Binds to Troponin-C by M, Ryan T, Tripet, Brian P, Pearlstone, Joyce R, Smillie, Lawrence B, Sykes, Brian D

“…The kinetics and energetics of the binding of three troponin-I peptides, corresponding to regions 96−131 (TnI96 - 131), 96−139 (TnI96 - 139), and 96−148 (TnI96…”
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Structure and Interaction Site of the Regulatory Domain of Troponin-C When Complexed with the 96−148 Region of Troponin-I by McKay, Ryan T, Pearlstone, Joyce R, Corson, David C, Gagné, Stéphane M, Smillie, Lawrence B, Sykes, Brian D

“…The structure of the regulatory domain of chicken skeletal troponin-C (residues 1−90) when complexed with the major inhibitory region (residues 96−148) of…”
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Skeletal muscle troponin C. Expression and purification of the recombinant intact protein and its isolated N- and C-domain fragments by Pearlstone, Joyce R, Smillie, Lawrence B

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NMR Studies of Ca2+ Binding to the Regulatory Domains of Cardiac and E41A Skeletal Muscle Troponin C Reveal the Importance of Site I to Energetics of the Induced Structural Changes by Li, Monica X, Gagné, Stéphane M, Spyracopoulos, Leo, Kloks, Cathelijne P. A. M, Audette, Gerald, Chandra, Murali, Solaro, R. John, Smillie, Lawrence B, Sykes, Brian D

“…Ca2+ binding to the N-domain of skeletal muscle troponin C (sNTnC) induces an “opening” of the structure [Gagné, S. M., et al. (1995) Nat. Struct. Biol. 2,…”
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Evidence for Two-Site Binding of Troponin I Inhibitory Peptides to the N and C Domains of Troponin C by Pearlstone, Joyce R, Smillie, Lawrence B

“…The interactions of two troponin I peptides, Ip1 (residues 96-116) and Ip2 (residues 104-116), with spectral probe mutants F29W and F105W of intact troponin C…”
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Polymerizability of rabbit skeletal tropomyosin: effects of enzymic and chemical modifications by Johnson, Peter, Smillie, Lawrence B

“…Polymerizability of tropomyosin was unaffected by the removal of the three terminal residues 282, 283, and 284 using carboxypeptidase A. However, when residue…”
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Calcium-Induced Dimerization of Troponin C: Mode of Interaction and Use of Trifluoroethanol as a Denaturant of Quaternary Structure by Slupsky, Carolyn M, Kay, Cyril M, Reinach, Fernando C, Smillie, Lawrence B, Sykes, Brian D

“…Protein aggregation can be a problem, especially as a large number of proteins become available for structural studies at fairly high concentrations using…”
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Biological Function and Site II Ca2+-induced Opening of the Regulatory Domain of Skeletal Troponin C Are Impaired by Invariant Site I or II Glu Mutations by Pearlstone, Joyce R., Chandra, Murali, Sorenson, Martha M., Smillie, Lawrence B.

“…To investigate the roles of site I and II invariant Glu residues 41 and 77 in the functional properties and calcium-induced structural opening of skeletal…”
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