Search Results - "Smallwood, Sébastien A"

Dynamic CpG island methylation landscape in oocytes and preimplantation embryos by Kelsey, Gavin, Smallwood, Sébastien A, Tomizawa, Shin-ichi, Krueger, Felix, Ruf, Nico, Carli, Natasha, Segonds-Pichon, Anne, Sato, Shun, Hata, Kenichiro, Andrews, Simon R

“…Elucidating how and to what extent CpG islands (CGIs) are methylated in germ cells is essential to understand genomic imprinting and epigenetic reprogramming…”
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A genome-scale map of DNA methylation turnover identifies site-specific dependencies of DNMT and TET activity by Ginno, Paul Adrian, Gaidatzis, Dimos, Feldmann, Angelika, Hoerner, Leslie, Imanci, Dilek, Burger, Lukas, Zilbermann, Frederic, Peters, Antoine H. F. M., Edenhofer, Frank, Smallwood, Sébastien A., Krebs, Arnaud R., Schübeler, Dirk

“…DNA methylation is considered a stable epigenetic mark, yet methylation patterns can vary during differentiation and in diseases such as cancer. Local levels…”
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BANP opens chromatin and activates CpG-island-regulated genes by Grand, Ralph S., Burger, Lukas, Gräwe, Cathrin, Michael, Alicia K., Isbel, Luke, Hess, Daniel, Hoerner, Leslie, Iesmantavicius, Vytautas, Durdu, Sevi, Pregnolato, Marco, Krebs, Arnaud R., Smallwood, Sébastien A., Thomä, Nicolas, Vermeulen, Michiel, Schübeler, Dirk

“…The majority of gene transcripts generated by RNA polymerase II in mammalian genomes initiate at CpG island (CGI) promoters 1 , 2 , yet our understanding of…”
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Genome-wide Bisulfite Sequencing in Zygotes Identifies Demethylation Targets and Maps the Contribution of TET3 Oxidation by Peat, Julian R., Dean, Wendy, Clark, Stephen J., Krueger, Felix, Smallwood, Sébastien A., Ficz, Gabriella, Kim, Jong Kyoung, Marioni, John C., Hore, Timothy A., Reik, Wolf

“…Fertilization triggers global erasure of paternal 5-methylcytosine as part of epigenetic reprogramming during the transition from gametic specialization…”
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Cooperation between HDAC3 and DAX1 mediates lineage restriction of embryonic stem cells by Olivieri, Daniel, Castelli, Eleonora, Kawamura, Yumiko K, Papasaikas, Panagiotis, Lukonin, Ilya, Rittirsch, Melanie, Hess, Daniel, Smallwood, Sébastien A, Stadler, Michael B, Peters, Antoine H F M, Betschinger, Joerg

“…Mouse embryonic stem cells (mESCs) are biased toward producing embryonic rather than extraembryonic endoderm fates. Here, we identify the mechanism of this…”
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Genome-wide analysis of DNA methylation in low cell numbers by reduced representation bisulfite sequencing by Smallwood, Sébastien A, Kelsey, Gavin

“…Development of high-throughput sequencing technologies now enables genome-wide analysis of DNA methylation of mammalian cells and tissues. Here, we present a…”
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De novo DNA methylation: a germ cell perspective by Smallwood, Sébastien A, Kelsey, Gavin

“…DNA methylation is a fundamentally important epigenetic modification of the mammalian genome that has widespread influences on gene expression. During…”
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Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity by Smallwood, Sébastien A, Lee, Heather J, Angermueller, Christof, Krueger, Felix, Saadeh, Heba, Peat, Julian, Andrews, Simon R, Stegle, Oliver, Reik, Wolf, Kelsey, Gavin

“…Single-cell bisulfite sequencing (scBS-seq) allows robust DNA methylation analysis in rare cells and heterogeneous populations. We report a single-cell…”
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Single-cell epigenomics: powerful new methods for understanding gene regulation and cell identity by Clark, Stephen J, Lee, Heather J, Smallwood, Sébastien A, Kelsey, Gavin, Reik, Wolf

“…Emerging single-cell epigenomic methods are being developed with the exciting potential to transform our knowledge of gene regulation. Here we review available…”
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Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity by Angermueller, Christof, Clark, Stephen J, Lee, Heather J, Macaulay, Iain C, Teng, Mabel J, Hu, Tim Xiaoming, Krueger, Felix, Smallwood, Sébastien A, Ponting, Chris P, Voet, Thierry, Kelsey, Gavin, Stegle, Oliver, Reik, Wolf

“…Parallel, genome-wide methylation and transcriptional profiling from a single cell (scM&T-seq) enables the discovery of associations between transcriptional…”
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Dynamic changes in histone modifications precede de novo DNA methylation in oocytes by Stewart, Kathleen R, Veselovska, Lenka, Kim, Jeesun, Huang, Jiahao, Saadeh, Heba, Tomizawa, Shin-ichi, Smallwood, Sébastien A, Chen, Taiping, Kelsey, Gavin

“…Erasure and subsequent reinstatement of DNA methylation in the germline, especially at imprinted CpG islands (CGIs), is crucial to embryogenesis in mammals…”
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Genome-wide base-resolution mapping of DNA methylation in single cells using single-cell bisulfite sequencing (scBS-seq) by Clark, Stephen J, Smallwood, Sébastien A, Lee, Heather J, Krueger, Felix, Reik, Wolf, Kelsey, Gavin

“…Single-cell bisulfite sequencing (scBS-seq) is a cost-effective method for surveying CpG methylation across genomes of single cells. It gives higher coverage…”
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The BTB-domain transcription factor ZBTB2 recruits chromatin remodelers and a histone chaperone during the exit from pluripotency by Olivieri, Daniel, Paramanathan, Sujani, Bardet, Anaïs F., Hess, Daniel, Smallwood, Sébastien A., Elling, Ulrich, Betschinger, Joerg

“…Transcription factors (TFs) harboring broad-complex, tramtrack, and bric-a-brac (BTB) domains play important roles in development and disease. These BTB…”
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Continuous Histone Replacement by Hira Is Essential for Normal Transcriptional Regulation and De Novo DNA Methylation during Mouse Oogenesis by Nashun, Buhe, Hill, Peter W.S., Smallwood, Sebastien A., Dharmalingam, Gopuraja, Amouroux, Rachel, Clark, Stephen J., Sharma, Vineet, Ndjetehe, Elodie, Pelczar, Pawel, Festenstein, Richard J., Kelsey, Gavin, Hajkova, Petra

“…The integrity of chromatin, which provides a dynamic template for all DNA-related processes in eukaryotes, is maintained through replication-dependent and…”
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Transcription and chromatin determinants of de novo DNA methylation timing in oocytes by Gahurova, Lenka, Tomizawa, Shin-Ichi, Smallwood, Sébastien A, Stewart-Morgan, Kathleen R, Saadeh, Heba, Kim, Jeesun, Andrews, Simon R, Chen, Taiping, Kelsey, Gavin

“…Gametogenesis in mammals entails profound re-patterning of the epigenome. In the female germline, DNA methylation is acquired late in oogenesis from an…”
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Deep sequencing and de novo assembly of the mouse oocyte transcriptome define the contribution of transcription to the DNA methylation landscape by Veselovska, Lenka, Smallwood, Sebastien A, Saadeh, Heba, Stewart, Kathleen R, Krueger, Felix, Maupetit-Méhouas, Stéphanie, Arnaud, Philippe, Tomizawa, Shin-Ichi, Andrews, Simon, Kelsey, Gavin

“…Previously, a role was demonstrated for transcription in the acquisition of DNA methylation at imprinted control regions in oocytes. Definition of the oocyte…”
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Transcription is required for establishment of germline methylation marks at imprinted genes by Chotalia, Mita, Smallwood, Sebastien A, Ruf, Nico, Dawson, Claire, Lucifero, Diana, Frontera, Marga, James, Katherine, Dean, Wendy, Kelsey, Gavin

“…Genomic imprinting requires the differential marking by DNA methylation of genes in male and female gametes. In the female germline, acquisition of methylation…”
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Low Input Whole-Genome Bisulfite Sequencing Using a Post-Bisulfite Adapter Tagging Approach by Peat, Julian R, Smallwood, Sébastien A

“…The epigenetic mark 5-methylcytosine confers heritable regulation of gene expression that can be dynamically modulated during transitions in cell fate. With…”
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Genome-Wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Approach by Lee, Heather J, Smallwood, Sébastien A

“…DNA methylation is an epigenetic mark implicated in the regulation of key biological processes. Using high-throughput sequencing technologies and…”
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Genome-Scale Oscillations in DNA Methylation during Exit from Pluripotency by Rulands, Steffen, Lee, Heather J, Clark, Stephen J, Angermueller, Christof, Smallwood, Sébastien A, Krueger, Felix, Mohammed, Hisham, Dean, Wendy, Nichols, Jennifer, Rugg-Gunn, Peter, Kelsey, Gavin, Stegle, Oliver, Simons, Benjamin D, Reik, Wolf

“…Pluripotency is accompanied by the erasure of parental epigenetic memory, with naïve pluripotent cells exhibiting global DNA hypomethylation both in vitro and…”
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