Search Results - "Smales, C Mark"

Strategies to control therapeutic antibody glycosylation during bioprocessing: Synthesis and separation by Edwards, Elizabeth, Livanos, Maria, Krueger, Anja, Dell, Anne, Haslam, Stuart M., Mark Smales, C., Bracewell, Daniel G.

“…Glycosylation can be a critical quality attribute in biologic manufacturing. In particular, it has implications on the half‐life, immunogenicity, and…”
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Analytics of host cell proteins (HCPs): lessons from biopharmaceutical mAb analysis for Gene therapy products by Bracewell, Daniel G, Smith, Victoria, Delahaye, Mike, Smales, C Mark

“…[Display omitted] •Host cell protein analysis in biopharma informs analysis in gene therapy products.•Developments in mass spectrometry HCP analysis of gene…”
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Isolation and characterisation of exosomes from Chinese hamster ovary (CHO) cells by Skrika-Alexopoulos, Eleftheria, Mark Smales, C

“…Exosomes have previously been isolated from Chinese hamster ovary (CHO) cells and their anti-apoptotic properties reported. However, to further facilitate the…”
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Control of translation elongation in health and disease by Knight, John R P, Garland, Gavin, Pöyry, Tuija, Mead, Emma, Vlahov, Nikola, Sfakianos, Aristeidis, Grosso, Stefano, De-Lima-Hedayioglu, Fabio, Mallucci, Giovanna R, von der Haar, Tobias, Smales, C Mark, Sansom, Owen J, Willis, Anne E

“…Regulation of protein synthesis makes a major contribution to post-transcriptional control pathways. During disease, or under stress, cells initiate processes…”
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Control and regulation of the cellular responses to cold shock: the responses in yeast and mammalian systems by Al-Fageeh, Mohamed B, Smales, C Mark

“…Although the cold-shock response has now been studied in a number of different organisms for several decades, it is only in the last few years that we have…”
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Evaluating data integrity in ribosome footprinting datasets through modelled polysome profiles by Hedayioglu, Fabio, Mead, Emma J, O'Connor, Patrick B F, Skiotys, Matas, Sansom, Owen J, Mallucci, Giovanna R, Willis, Anne E, Baranov, Pavel V, Smales, C Mark, von der Haar, Tobias

“…The assessment of transcriptome-wide ribosome binding to mRNAs is useful for studying the dynamic regulation of protein synthesis. Two methods frequently…”
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Biological insights into the expression of translation initiation factors from recombinant CHOK1SV cell lines and their relationship to enhanced productivity by Mead, Emma J, Masterton, Rosalyn J, Feary, Marc, Obrezanova, Olga, Zhang, Lin, Young, Robert, Smales, C Mark

“…Translation initiation is on the critical pathway for the production of monoclonal antibodies (mAbs) by mammalian cells. Formation of a closed loop structure…”
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Glycosylation of Trypanosoma cruzi TcI antigen reveals recognition by chagasic sera by Murphy, Niamh, Rooney, Barrie, Bhattacharyya, Tapan, Triana-Chavez, Omar, Krueger, Anja, Haslam, Stuart M., O’Rourke, Victoria, Pańczuk, Magdalena, Tsang, Jemima, Bickford-Smith, Jack, Gilman, Robert H., Tetteh, Kevin, Drakeley, Chris, Smales, C. Mark, Miles, Michael A.

“…Chagas disease is considered the most important parasitic disease in Latin America. The protozoan agent, Trypanosoma cruzi , comprises six genetic lineages,…”
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Developments in the production of mucosal antibodies in plants by Vasilev, Nikolay, Smales, C. Mark, Schillberg, Stefan, Fischer, Rainer, Schiermeyer, Andreas

“…Recombinant mucosal antibodies represent attractive target molecules for the development of next generation biopharmaceuticals for passive immunization against…”
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Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation by Knight, John R P, Bastide, Amandine, Roobol, Anne, Roobol, Jo, Jackson, Thomas J, Utami, Wahyu, Barrett, David A, Smales, C Mark, Willis, Anne E

“…Cells respond to external stress conditions by controlling gene expression, a process which occurs rapidly via post-transcriptional regulation at the level of…”
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Characterization of AAV vectors: A review of analytical techniques and critical quality attributes by Kontogiannis, Theodoros, Braybrook, Julian, McElroy, Christopher, Foy, Carole, Whale, Alexandra S., Quaglia, Milena, Smales, C Mark

“…Standardized evaluation of adeno-associated virus (AAV) vector products for biotherapeutic application is essential to ensure the safety and efficacy of gene…”
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Cold-inducible RNA binding protein (CIRP) expression is modulated by alternative mRNAs by Al-Fageeh, Mohamed B, Smales, C Mark

“…Cold-inducible RNA binding protein (CIRP) is a mammalian protein whose expression is up-regulated in response to mild hypothermia. Although the exact function…”
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Cooling-induced SUMOylation of EXOSC10 down-regulates ribosome biogenesis by Knight, John R P, Bastide, Amandine, Peretti, Diego, Roobol, Anne, Roobol, Jo, Mallucci, Giovanna R, Smales, C Mark, Willis, Anne E

“…The RNA exosome is essential for 3' processing of functional RNA species and degradation of aberrant RNAs in eukaryotic cells. Recent reports have defined the…”
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Editorial: Characterization of biotherapeutic products by Rathore, Anurag S., Smales, C. Mark, Guttman, Andras

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Expression of Trypanosoma brucei gambiense Antigens in Leishmania tarentolae. Potential for Use in Rapid Serodiagnostic Tests (RDTs) by Rooney, Barrie, Piening, Turid, Büscher, Philippe, Rogé, Stijn, Smales, C Mark

“…The development of rapid serodiagnostic tests for sleeping sickness and other diseases caused by kinetoplastids relies on the affordable production of…”
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ATR (ataxia telangiectasia mutated- and Rad3-related kinase) is activated by mild hypothermia in mammalian cells and subsequently activates p53 by Roobol, Anne, Roobol, Jo, Carden, Martin J, Bastide, Amandine, Willis, Anne E, Dunn, Warwick B, Goodacre, Royston, Smales, C Mark

“…In vitro cultured mammalian cells respond to mild hypothermia (27-33 °C) by attenuating cellular processes and slowing and arresting the cell cycle. The…”
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Translation of in vitro -transcribed RNA therapeutics by von der Haar, Tobias, Mulroney, Thomas E, Hedayioglu, Fabio, Kurusamy, Sathishkumar, Rust, Maria, Lilley, Kathryn S, Thaventhiran, James E, Willis, Anne E, Smales, C Mark

“…transcribed, modified messenger RNAs (IVTmRNAs) have been used to vaccinate billions of individuals against the SARS-CoV-2 virus, and are currently being…”
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Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis by Parsons, Joshua B., Dinesh, Sriramulu D., Deery, Evelyne, Leech, Helen K., Brindley, Amanda A., Heldt, Dana, Frank, Steffanie, Smales, C. Mark, Lünsdorf, Heinrich, Rambach, Alain, Gass, Mhairi H., Bleloch, Andrew, McClean, Kirsty J., Munro, Andrew W., Rigby, Stephen E.J., Warren, Martin J., Prentice, Michael B.

“…Many heterotrophic bacteria have the ability to make polyhedral structures containing metabolic enzymes that are bounded by a unilamellar protein shell…”
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Alternative Promoters Regulate Cold Inducible RNA-Binding (CIRP) Gene Expression and Enhance Transgene Expression in Mammalian Cells by Al-Fageeh, Mohamed B, Smales, C. Mark

“…The use of a temperature shift cultivation to enhance recombinant protein yield is widely utilised in the bioprocessing industry. The responses of mammalian…”
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Defining lncRNAs Correlated with CHO Cell Growth and IgG Productivity by RNA-Seq by Vito, Davide, Eriksen, Jens Christian, Skjødt, Christian, Weilguny, Dietmar, Rasmussen, Søren K., Smales, C. Mark

“…How the long non-coding RNA (lncRNA) genome in recombinant protein producing Chinese hamster ovary (CHO) cell lines relates to phenotype is not well described…”
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