Search Results - "Slorach, Euan M"

Mapping the genomic landscape of CRISPR–Cas9 cleavage by Cameron, Peter, Fuller, Chris K, Donohoue, Paul D, Jones, Brittnee N, Thompson, Matthew S, Carter, Matthew M, Gradia, Scott, Vidal, Bastien, Garner, Elizabeth, Slorach, Euan M, Lau, Elaine, Banh, Lynda M, Lied, Alexandra M, Edwards, Leslie S, Settle, Alexander H, Capurso, Daniel, Llaca, Victor, Deschamps, Stéphane, Cigan, Mark, Young, Joshua K, May, Andrew P

“…SITE-Seq probes Cas9 cleavage sites in vitro and returns a comprehensive list of off-target sites at different Cas9–sgRNA concentrations. RNA-guided…”
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GATA-3 Maintains the Differentiation of the Luminal Cell Fate in the Mammary Gland by Kouros-Mehr, Hosein, Slorach, Euan M., Sternlicht, Mark D., Werb, Zena

“…The GATA family of transcription factors plays fundamental roles in cell-fate specification. However, it is unclear if these genes are necessary for the…”
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Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality by Briner, Alexandra E., Donohoue, Paul D., Gomaa, Ahmed A., Selle, Kurt, Slorach, Euan M., Nye, Christopher H., Haurwitz, Rachel E., Beisel, Chase L., May, Andrew P., Barrangou, Rodolphe

“…The RNA-guided Cas9 endonuclease specifically targets and cleaves DNA in a sequence-dependent manner and has been widely used for programmable genome editing…”
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GATA-3 Links Tumor Differentiation and Dissemination in a Luminal Breast Cancer Model by Kouros-Mehr, Hosein, Bechis, Seth K., Slorach, Euan M., Littlepage, Laurie E., Egeblad, Mikala, Ewald, Andrew J., Pai, Sung-Yun, Ho, I-Cheng, Werb, Zena

“…How breast cancers are able to disseminate and metastasize is poorly understood. Using a hyperplasia transplant system, we show that tumor dissemination and…”
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The Transcriptional Repressor ZNF503/Zeppo2 Promotes Mammary Epithelial Cell Proliferation and Enhances Cell Invasion by Shahi, Payam, Slorach, Euan M., Wang, Chih-Yang, Chou, Jonathan, Lu, Angela, Ruderisch, Aline, Werb, Zena

“…The NET (nocA, Nlz, elB, TLP-1) subfamily of zinc finger proteins is an important mediator during developmental processes. The evolutionary conserved zinc…”
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Zeppo1 is a novel metastasis promoter that represses E-cadherin expression and regulates p120-catenin isoform expression and localization by Slorach, Euan M, Chou, Jonathan, Werb, Zena

“…Amplification of 8p11-12 in human breast cancers is associated with increased proliferation and tumor grade and reduced metastasis-free patient survival. We…”
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Harnessing type I CRISPR–Cas systems for genome engineering in human cells by Cameron, Peter, Coons, Mary M., Klompe, Sanne E., Lied, Alexandra M., Smith, Stephen C., Vidal, Bastien, Donohoue, Paul D., Rotstein, Tomer, Kohrs, Bryan W., Nyer, David B., Kennedy, Rachel, Banh, Lynda M., Williams, Carolyn, Toh, Mckenzi S., Irby, Matthew J., Edwards, Leslie S., Lin, Chun-Han, Owen, Arthur L. G., Künne, Tim, van der Oost, John, Brouns, Stan J. J., Slorach, Euan M., Fuller, Chris K., Gradia, Scott, Kanner, Steven B., May, Andrew P., Sternberg, Samuel H.

“…Type I CRISPR–Cas systems are the most abundant adaptive immune systems in bacteria and archaea 1 , 2 . Target interference relies on a multi-subunit,…”
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Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells by Donohoue, Paul D., Pacesa, Martin, Lau, Elaine, Vidal, Bastien, Irby, Matthew J., Nyer, David B., Rotstein, Tomer, Banh, Lynda, Toh, Mckenzi S., Gibson, Jason, Kohrs, Bryan, Baek, Kevin, Owen, Arthur L.G., Slorach, Euan M., van Overbeek, Megan, Fuller, Christopher K., May, Andrew P., Jinek, Martin, Cameron, Peter

“…The off-target activity of the CRISPR-associated nuclease Cas9 is a potential concern for therapeutic genome editing applications. Although high-fidelity Cas9…”
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ZNF503/Zpo2 drives aggressive breast cancer progression by down-regulation of GATA3 expression by Shahi, Payam, Wang, Chih-Yang, Lawson, Devon A., Slorach, Euan M., Lu, Angela, Yu, Ying, Lai, Ming-Derg, Velozo, Hugo Gonzalez, Werb, Zena

“…The transcription factor GATA3 is the master regulator that drives mammary luminal epithelial cell differentiation and maintains mammary gland homeostasis…”
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Author Correction: Mapping the genomic landscape of CRISPR–Cas9 cleavage by Cameron, Peter, Fuller, Chris K., Donohoue, Paul D., Jones, Brittnee N., Thompson, Matthew S., Carter, Matthew M., Gradia, Scott, Vidal, Bastien, Garner, Elizabeth, Slorach, Euan M., Lau, Elaine, Banh, Lynda M., Lied, Alexandra M., Edwards, Leslie S., Settle, Alexander H., Capurso, Daniel, Llaca, Victor, Deschamps, Stéphane, Cigan, Mark, Young, Joshua K., May, Andrew P.

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ZNF50 3/ Zpo2 drives aggressive breast cancer progression by down-regulation of GATA3 expression by Shahi, Payam, Wang, Chih-Yang, Lawson, Devon A, Slorach, Euan M, Lu, Angela, Yu, Ying, Lai, Ming-Derg, Gonzalez Velozo, Hugo, Werb, Zena

“…The transcription factor GATA3 is the master regulator that drives mammary luminal epithelial cell differentiation and maintains mammary gland homeostasis…”
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Epithelial Morphogenesis: Netrin Comes to a Sticky and Terminal End by Slorach, Euan M., Werb, Zena

“…Netrin and its receptor Neogenin are thought to be regulators of axonal guidance in the nervous system. A recent report suggests they also play a role in…”
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Abstract 2475: Zpo2 promotes aggressive breast cancer development through downregulation of GATA3 by Shahi, Payam, Slorach, Euan M., Chou, Jonathan, Lawson, Devon, Yu, Ying, Werb, Zena

“…Abstract Metastatic breast cancer is the major cause of poor prognosis and death in women. The transcription factor GATA3 is a key mediator of luminal cell…”
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Highly Specific Cas9 and Cas12a Engineering of Human T Cells for Generation of Novel Allogeneic Cell Therapies by Donohoue, Paul, Pacesa, Martin, Lau, Elaine, Vidal, Bastien, Irby, Matthew J, Nyer, David B, Rotstein, Tomer, Loncaric, Mikey, Ciminelli, Emma, Mause, Erica Vander, Munoz, Tony, Banh, Lynda, Toh, McKenzi S, Churchward, Glen, Gonzalez, Rachel, Gibson, Jason, Kohrs, Bryan, Baek, Kevin, Owen, Arthur L.G., Slorach, Euan M, van Overbeek, Megan, May, Andrew P, Garner, Elizabeth, Jinek, Martin, Cameron, Peter

“…CRISPR-based genome editing of primary human T cells has the potential to revolutionize disease-modifying therapies. However, substantial improvements in…”
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A mouse model of intestinal stem cell function and regeneration by Slorach, E M, Campbell, F C, Dorin, J R

“…We present here an in vivo mouse model for intestinal stem cell function and differentiation that uses postnatal intestinal epithelial cell aggregates to…”
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Intestinal epithelial stem cells as targets for cf gene therapy by Slorach, Euan M

“…As a first step towards mutation correction by homologous recombination I have constructed a targeting vector which contains wild type Cftr genomic DNA…”
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Human repeat-mediated integration of selectable markers into somatic cell hybrids by Watson, J E, Slorach, E M, Maule, J, Lawson, D, Porteous, D J, Brookes, A J

“…We describe a strategy to introduce preferentially the dominant selectable marker neoR into the human chromosome within a monochromosome hybrid cell line…”
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Catch-linker + PCR labeling: a simple method to generate fluorescence in situ hybridization probes from yeast artificial chromosomes by Shibasaki, Y, Maule, J C, Devon, R S, Slorach, E M, Gosden, J R, Porteous, D J, Brookes, A J

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A Contiguous Clone Map over 3 Mb on the Long Arm of Chromosome 11 across a Balanced Translocation Associated with Schizophrenia by Evans, Kathryn L., Brown, John, Shibasaki, Yoshiro, Devon, Rebecca S., He, Lin, Arveiler, Benoit, Christie, Sheila, Maule, John C., Baillie, David, Slorach, Euan M., Anderson, Susan M., Gosden, John R., Petit, Joelle, Weith, Andreas, Gosden, Christine M., Blackwood, Douglas H.R., St. Clair, David M., Muir, Walter J., Brookes, Anthony J., Porteous, David J.

“…Forty-nine clones derived by microdissection of a schizophrenia-associated t(1;11)(q42.1;q14.3) breakpoint region have been assigned by somatic cell hybrid…”
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Regional localisation of 19 brain expressed sequence tags to human chromosome 11 using PCR amplification of somatic cell hybrid DNAs by Slorach, E M, Polymeropoulos, M H, Evans, K L, Seawright, A, Fletcher, J M, Porteous, D J, Brookes, A J

“…Expressed sequence tags (ESTs) provide an efficient route to the identification of genes involved in normal development and in disease. PCR amplification of…”
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