Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of Polyscias filicifolia was developed. The phenolic compound content in P. filicifolia has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses...

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Bibliographic Details
Published in:	Acta Societatis Botanicorum Poloniae Vol. 87; no. 2; p. 1
Main Authors:	Sliwinska, Anita Agnieszka, Syklowska-Baranek, Katarzyna, Kosmider, Anita, Granica, Sebastian, Miszczak, Karolina, Nowicka, Grazyna, Kasztelan, Adrian, Podsadni, Piotr, Turlo, Jadwiga, Pietrosiuk, Agnieszka
Format:	Journal Article
Language:	English
Published:	Polskie Towarzystwo Botaniczne 22-06-2018 Polish Botanical Society
Subjects:	A549 cells Analysis antioxidant capacity Antioxidants (Nutrients) Araliaceae Botanical research cytotoxic activity HaCaT Phenol (Compound) Plant extracts polyphenols Polyscias filicifolia
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Summary:	In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of Polyscias filicifolia was developed. The phenolic compound content in P. filicifolia has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses of 50, 100, or 200 [micro]M. HPLC-UV-VIS and LC-MS techniques were used for the determination of chlorogenic, caffeic, and ferulic acids. The total phenolics and flavonoids were quantified, and the antioxidant capacity of plant extracts was determined using DPPH and ABTS methods. Finally, the cytotoxic activity of P. filicifolia extracts in normal (HaCaT) and cancer (A549) cells was investigated. Further, the effect of the extracts on cisplatin cytotoxicity was assessed. The elicitors significantly enhanced phenolic production compared to that in untreated shoots and leaves of intact plants. Chlorogenic acid was the most abundant compound with the highest yield of 5.03 [+ or -]0.25 mg/g DW after treatment with 50 [micro]M SA. The total flavonoid and phenolic content was significantly and dose-dependently influenced by JM. The highest antioxidant capacity was noted in extracts derived from shoots grown on media supplemented with 50 [micro]M SA and 200 [micro]M JM; these doses were used for further cytotoxic activity investigations. The extracts from JM or SA treatments reduced cancer cell viability and increased their mortality, whereas the extract from JM treatment exhibited protective effect on normal cells. Moreover, the comparison of cytotoxic properties of plant extracts and cisplatin indicated that plant phenolic compounds in combination with anticancer drugs could reduce the detrimental effect of the latter on human cells. Keywords Polyscias filicifolia; polyphenols; antioxidant capacity; cytotoxic activity; HaCaT; A549 cells
ISSN:	0001-6977 2083-9480
DOI:	10.5586/asbp.3586