Application of Advanced Non-Linear Spectral Decomposition and Regression Methods for Spectroscopic Analysis of Targeted and Non-Targeted Irradiation Effects in an In-Vitro Model

Irradiation of the tumour site during treatment for cancer with external-beam ionising radiation results in a complex and dynamic series of effects in both the tumour itself and the normal tissue which surrounds it. The development of a spectral model of the effect of each exposure and interaction m...

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Bibliographic Details
Published in:	International journal of molecular sciences Vol. 23; no. 21; p. 12986
Main Authors:	Slattery, Ciara, Nguyen, Khanh, Shields, Laura, Vega-Carrascal, Isabel, Singleton, Sean, Lyng, Fiona M., McClean, Brendan, Meade, Aidan D.
Format:	Journal Article
Language:	English
Published:	Basel MDPI AG 01-11-2022 MDPI
Subjects:	Cancer therapies Cell culture Cell differentiation Cell fate Communication Data acquisition Decomposition DNA damage Dosimetry Exposure extreme gradient boosting regression (XGBR) Fourier transform infrared microspectroscopy (FTIRM) Gamma rays Infrared analysis Infrared imaging Ionizing radiation out of field effects principal components analysis (PCA) Prostate cancer Radiation dosage Radiation effects Radiation therapy Spectroscopic analysis Spectrum analysis support vector machine (SVM) t-stochastic neighbourhood embedding (t-SNE) Tumors X-rays
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Summary:	Irradiation of the tumour site during treatment for cancer with external-beam ionising radiation results in a complex and dynamic series of effects in both the tumour itself and the normal tissue which surrounds it. The development of a spectral model of the effect of each exposure and interaction mode between these tissues would enable label free assessment of the effect of radiotherapeutic treatment in practice. In this study Fourier transform Infrared microspectroscopic imaging was employed to analyse an in-vitro model of radiotherapeutic treatment for prostate cancer, in which a normal cell line (PNT1A) was exposed to low-dose X-ray radiation from the scattered treatment beam, and also to irradiated cell culture medium (ICCM) from a cancer cell line exposed to a treatment relevant dose (2 Gy). Various exposure modes were studied and reference was made to previously acquired data on cellular survival and DNA double strand break damage. Spectral analysis with manifold methods, linear spectral fitting, non-linear classification and non-linear regression approaches were found to accurately segregate spectra on irradiation type and provide a comprehensive set of spectral markers which differentiate on irradiation mode and cell fate. The study demonstrates that high dose irradiation, low-dose scatter irradiation and radiation-induced bystander exposure (RIBE) signalling each produce differential effects on the cell which are observable through spectroscopic analysis.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms232112986