Pathotype-specific QTL for stem rust resistance in Lolium perenne

A genetic map populated with RAD and SSR markers was created from F1 progeny of a stem rust-susceptible and stem rust-resistant parent of perennial ryegrass ( Lolium perenne ). The map supplements a previous map of this population by having markers in common with several other Lolium spp. maps inclu...

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Bibliographic Details
Published in:	Theoretical and applied genetics Vol. 126; no. 5; pp. 1213 - 1225
Main Authors:	Pfender, W. F., Slabaugh, M. E.
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer-Verlag 01-05-2013 Springer Nature B.V
Subjects:	Agriculture Basidiomycota - genetics Basidiomycota - immunology Basidiomycota - pathogenicity Biochemistry Biomedical and Life Sciences Biotechnology Chromosome Mapping Chromosomes, Plant Disease Resistance - genetics DNA, Plant - genetics Genes, Plant Genetic Linkage Genetic Markers Immunity, Innate - genetics Life Sciences Lolium Lolium - genetics Lolium - immunology Lolium - microbiology Lolium perenne Original Paper Pathogens Phenotype Plant Biochemistry Plant Breeding/Biotechnology Plant Diseases - genetics Plant Diseases - microbiology Plant Genetics and Genomics Plant Stems - genetics Plant Stems - immunology Plant Stems - microbiology Puccinia graminis Quantitative Trait Loci Soil sciences Virulence United States > US Perennial Ryegrass Rust Resistance Stem Rust Quantitative Trait Locus Quantitative Trait Locus Analysis
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Summary:	A genetic map populated with RAD and SSR markers was created from F1 progeny of a stem rust-susceptible and stem rust-resistant parent of perennial ryegrass ( Lolium perenne ). The map supplements a previous map of this population by having markers in common with several other Lolium spp. maps including EST-SSR anchor markers from a consensus map published by other researchers. A QTL analysis was conducted with disease severity and infection type data obtained by controlled inoculation of the population with each of two previously characterized pathotypes of Puccinia graminis subsp. graminicola that differ in virulence to different host plant genotypes in the F1 population. Each pathotype activated a specific QTL on one linkage group (LG): qLpPg1 on LG7 for pathotype 101, or qLpPg2 on LG1 for pathotype 106. Both pathotypes also activated a third QTL in common, qLpPg3 on LG6. Anchor markers, present on a consensus map, were located in proximity to each of the three QTL. These QTL had been detected also in previous experiments in which a genetically heterogeneous inoculum of the stem rust pathogen activated all three QTL together. The results of this and a previous study are consistent with the involvement of the pathotype-specific QTL in pathogen recognition and the pathotype-nonspecific QTL in a generalized resistance response. By aligning the markers common to other published reports, it appears that two and possibly all three of the stem rust QTL reported here are in the same general genomic regions containing some of the L. perenne QTL reported to be activated in response to the crown rust pathogen ( P. coronata ).
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0040-5752 1432-2242
DOI:	10.1007/s00122-013-2048-2