Search Results - "Skowronek, Krzysztof J"

The mitotic kinesin-14 Ncd drives directional microtubule-microtubule sliding by Diez, Stefan, Fink, Gero, Hajdo, Lukasz, Skowronek, Krzysztof J, Reuther, Cordula, Kasprzak, Andrzej A

“…During mitosis and meiosis, the bipolar spindle facilitates chromosome segregation through microtubule sliding as well as microtubule growth and shrinkage…”
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Sequence-specific cleavage of dsRNA by Mini-III RNase by Głów, Dawid, Pianka, Dariusz, Sulej, Agata A, Kozłowski, Łukasz P, Czarnecka, Justyna, Chojnowski, Grzegorz, Skowronek, Krzysztof J, Bujnicki, Janusz M

“…Ribonucleases (RNases) play a critical role in RNA processing and degradation by hydrolyzing phosphodiester bonds (exo- or endonucleolytically). Many RNases…”
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Delineation of structural domains and identification of functionally important residues in DNA repair enzyme exonuclease VII by Poleszak, Katarzyna, Kaminska, Katarzyna H, Dunin-Horkawicz, Stanislaw, Lupas, Andrei, Skowronek, Krzysztof J, Bujnicki, Janusz M

“…Exonuclease VII (ExoVII) is a bacterial nuclease involved in DNA repair and recombination that hydrolyses single-stranded DNA. ExoVII is composed of two…”
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Interactions between Subunits in Heterodimeric Ncd Molecules by Kocik, Elzbieta, Skowronek, Krzysztof J., Kasprzak, Andrzej A.

“…The nonprocessive minus-end-directed kinesin-14 Ncd is involved in the organization of the microtubule (MT) network during mitosis. Only one of the two motor…”
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Direct interaction between prion protein and tubulin by Nieznanski, Krzysztof, Nieznanska, Hanna, Skowronek, Krzysztof J., Osiecka, Katarzyna M., Stepkowski, Dariusz

“…Recently published data show that the prion protein in its cellular form (PrP C) is a component of multimolecular complexes. In this report, zero-length…”
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Identification of a new subfamily of HNH nucleases and experimental characterization of a representative member, HphI restriction endonuclease by Cymerman, Iwona A., Obarska, Agnieszka, Skowronek, Krzysztof J., Lubys, Arvydas, Bujnicki, Janusz M.

“…The restriction endonuclease (REase) R. HphI is a Type IIS enzyme that recognizes the asymmetric target DNA sequence 5′‐GGTGA‐3′ and in the presence of Mg2+…”
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Crystal Structure and Directed Evolution of Specificity of NlaIV Restriction Endonuclease by Czapinska, Honorata, Siwek, Wojciech, Szczepanowski, Roman H., Bujnicki, Janusz M., Bochtler, Matthias, Skowronek, Krzysztof J.

“…Specificity engineering is challenging and particularly difficult for enzymes that have the catalytic machinery and specificity determinants in close…”
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Sequence-specific cleavage of the RNA strand in DNA-RNA hybrids by the fusion of ribonuclease H with a zinc finger by Sulej, Agata A, Tuszynska, Irina, Skowronek, Krzysztof J, Nowotny, Marcin, Bujnicki, Janusz M

“…Ribonucleases (RNases) are valuable tools applied in the analysis of RNA sequence, structure and function. Their substrate specificity is limited to…”
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Identification of protein structural elements responsible for the diversity of sequence preferences among Mini-III RNases by Głów, Dawid, Kurkowska, Małgorzata, Czarnecka, Justyna, Szczepaniak, Krzysztof, Pianka, Dariusz, Kappert, Verena, Bujnicki, Janusz M., Skowronek, Krzysztof J.

“…Many known endoribonucleases select their substrates based on the presence of one or a few specific nucleotides at or near the cleavage site. In some cases,…”
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Tau inhibits tubulin oligomerization induced by prion protein by Osiecka, Katarzyna M., Nieznanska, Hanna, Skowronek, Krzysztof J., Jozwiak, Jolanta, Nieznanski, Krzysztof

“…In previous studies we have demonstrated that prion protein (PrP) interacts with tubulin and disrupts microtubular cytoskeleton by inducing tubulin…”
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Prion protein region 23-32 interacts with tubulin and inhibits microtubule assembly by Osiecka, Katarzyna M., Nieznanska, Hanna, Skowronek, Krzysztof J., Karolczak, Justyna, Schneider, Gabriela, Nieznanski, Krzysztof

“…In previous studies we have demonstrated that prion protein (PrP) binds directly to tubulin and this interaction leads to the inhibition of microtubule…”
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Sequence-specific endoribonucleases by Głów, Dawid, Nowacka, Martyna, Skowronek, Krzysztof J, Bujnicki, Janusz M

“…Ribonucleases are nucleolytic enzymes that commonly occur in living organisms and act by cleaving RNA molecules. These enzymes are involved in basic cellular…”
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Theoretical model of restriction endonuclease HpaI in complex with DNA, predicted by fold recognition and validated by site-directed mutagenesis by Skowronek, Krzysztof J., Kosinski, Jan, Bujnicki, Janusz M.

“…Type II restriction enzymes are commercially important deoxyribonucleases and very attractive targets for protein engineering of new specificities. At the same…”
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Subunits interactions in kinesin motors by Skowronek, Krzysztof J., Kocik, Elzbieta, Kasprzak, Andrzej A.

“…Kinesins form a large and diverse superfamily of proteins involved in numerous important cellular processes. The majority of them are molecular motors moving…”
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Inference of relationships in the ‘twilight zone’ of homology using a combination of bioinformatics and site-directed mutagenesis: a case study of restriction endonucleases Bsp6I and PvuII by Pawlak, Sebastian D., Radlinska, Monika, Chmiel, Agnieszka A., Bujnicki, Janusz M., Skowronek, Krzysztof J.

“…Thus far, identification of functionally important residues in Type II restriction endonucleases (REases) has been difficult using conventional methods. Even…”
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A theoretical model of restriction endonuclease NlaIV in complex with DNA, predicted by fold recognition and validated by site-directed mutagenesis and circular dichroism spectroscopy by Chmiel, Agnieszka A., Radlinska, Monika, Pawlak, Sebastian D., Krowarsch, Daniel, Bujnicki, Janusz M., Skowronek, Krzysztof J.

“…Restriction enzymes (REases) are commercial reagents commonly used in DNA manipulations and mapping. They are regarded as very attractive models for studying…”
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A homology model of restriction endonuclease SfiI in complex with DNA by Chmiel, Agnieszka A, Bujnicki, Janusz M, Skowronek, Krzysztof J

“…Restriction enzymes (REases) are commercial reagents commonly used in recombinant DNA technologies. They are attractive models for studying protein-DNA…”
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Laboratory scale structural genomics by Segelke, Brent W, Schafer, Johana, Coleman, Matthew A, Lekin, Tim P, Toppani, Dominique, Skowronek, Krzysztof J, Kantardjieff, Katherine A, Rupp, Bernhard

“…At Lawrence Livermore National Laboratory, the development of the TB structural genomics consortium crystallization facility has paralleled several local…”
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