Search Results - "Skerra, A."

Anticalins: Exploiting a non-Ig scaffold with hypervariable loops for the engineering of binding proteins by Richter, A., Eggenstein, E., Skerra, A.

“…Antibodies, which can recognize a plethora of possible antigens, have been considered as a paradigm of protein engineering performed by nature itself…”
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PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins by Schlapschy, Martin, Binder, Uli, Börger, Claudia, Theobald, Ina, Wachinger, Klaus, Kisling, Sigrid, Haller, Dirk, Skerra, Arne

“…A major limitation of biopharmaceutical proteins is their fast clearance from circulation via kidney filtration, which strongly hampers efficacy both in animal…”
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Efficient secretory production of proline/alanine/serine (PAS) biopolymers in Corynebacterium glutamicum yielding a monodisperse biological alternative to polyethylene glycol (PEG) by Friedrich, L, Kikuchi, Y, Matsuda, Y, Binder, U, Skerra, A

“…Abstract Background PAS biopolymers are recombinant polypeptides comprising the small uncharged l -amino acids Pro, Ala and/or Ser which resemble the widely…”
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Molecular recognition of structurally disordered Pro/Ala-rich sequences (PAS) by antibodies involves an Ala residue at the hot spot of the epitope by Schilz, J., Binder, U., Friedrich, L., Gebauer, M., Lutz, C., Schlapschy, M., Schiefner, A., Skerra, A.

“…[Display omitted] •Generation of MAbs against intrinsically disordered Pro/Ala-rich sequences (PAS).•Elucidation of four crystal structures in complex with…”
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Structural plasticity in the loop region of engineered lipocalins with novel ligand specificities, so-called Anticalins by Achatz, S., Jarasch, A., Skerra, A.

“…[Display omitted] •The lipocalins exhibit four structurally variable loops at one end of a β-barrel.•Binding sites for diverse ligands occur in the natural…”
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Anti-fibrotic Effects of CXCR4-Targeting i-body AD-114 in Preclinical Models of Pulmonary Fibrosis by Griffiths, K., Habiel, D. M., Jaffar, J., Binder, U., Darby, W. G., Hosking, C. G., Skerra, A., Westall, G. P., Hogaboam, C. M., Foley, M.

“…Idiopathic pulmonary fibrosis (IPF) is a chronic fibrotic lung disease that is prevalent in individuals >50 years of age, with a median survival of 3–5 years…”
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Lipocalins in drug discovery: From natural ligand-binding proteins to ‘anticalins’ by Schlehuber, Steffen, Skerra, Arne

“…Lipocalins, an abundant family of ligand-binding and -transport proteins, have attracted attention as potential drugs, either based on their natural binding…”
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engineered lipocalin specific for CTLA-4 reveals a combining site with structural and conformational features similar to antibodies by Schönfeld, D, Matschiner, G, Chatwell, L, Trentmann, S, Gille, H, Hülsmeyer, M, Brown, N, Kaye, P.M, Schlehuber, S, Hohlbaum, A.M, Skerra, A

“…Biomolecular reagents that enable the specific molecular recognition of proteins play a crucial role in basic research as well as medicine. Up to now,…”
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Structure-guided engineering of Anticalins with improved binding behavior and biochemical characteristics for application in radio-immuno imaging and/or therapy by Eggenstein, E., Eichinger, A., Kim, H.-J., Skerra, A.

“…Modern strategies in radio-immuno therapy and in vivo imaging require robust, small, and specific ligand-binding proteins. In this context we have previously…”
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Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification by Voss, S, Skerra, A

“…The Strep-tag, an artificial peptide ligand of streptavidin, has gained use as an affinity handle for the purification and detection of recombinant fusion…”
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Regeneration of Lesioned Corticospinal Tract Fibers in the Adult Rat Induced by a Recombinant, Humanized IN-1 Antibody Fragment by Brosamle, Christian, Huber, Andrea B, Fiedler, Markus, Skerra, Arne, Schwab, Martin E

“…Axons in the CNS of higher vertebrates generally fail to regenerate after injury. This lack of regeneration is crucially influenced by neurite growth…”
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Binding proteins from alternative scaffolds by Nygren, Per-Åke, Skerra, Arne

“…The use of so-called protein scaffolds for the generation of novel binding proteins via combinatorial engineering has recently emerged as a powerful…”
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Duocalins: engineered ligand-binding proteins with dual specificity derived from the lipocalin fold by Schlehuber, S, Skerra, A

“…Anticalins comprise a novel class of receptor proteins with predetermined ligand specificities which were engineered using the lipocalin fold. Attractive…”
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Ultrafast Electron Transfer in the Complex between Fluorescein and a Cognate Engineered Lipocalin Protein, a So-Called Anticalin by Götz, M, Hess, S, Beste, G, Skerra, A, Michel-Beyerle, M. E

“…Anticalins are a novel class of engineered ligand-binding proteins with tailored specificities derived from the lipocalin scaffold. The anticalin FluA…”
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An engineered IN-1 Fab fragment with improved affinity for the Nogo-A axonal growth inhibitor permits immunochemical detection and shows enhanced neutralizing activity by Fiedler, M., Horn, C., Bandtlow, C., Schwab, M.E., Skerra, A.

“…The myelin axonal growth inhibitor NI-220/250 (Nogo-A) has attracted considerable attention in elucidating the mechanisms that account for the lack of…”
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Glutathione S‐transferase can be used as a C‐terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli by Tudyka, Tatjana, Skerra, Arne

“…Glutathione S‐transferase (GST) from Schistosoma japonicum, which is widely used for the production of fusion proteins in the cytoplasm of Escherichia coli,…”
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Functional humanization of an anti-CD30 Fab fragment for the immunotherapy of Hodgkin's lymphoma using an in vitro evolution approach by Schlapschy, Martin, Gruber, Helga, Gresch, Oliver, Schäfer, Claudia, Renner, Christoph, Pfreundschuh, Michael, Skerra, Arne

“…CD30, the so-called Reed–Sternberg antigen, constitutes a promising cell-specific target for the treatment of Hodgkin's lymphoma. Starting from the previously…”
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The periplasmic E. coli chaperone Skp is a trimer in solution: biophysical and preliminary crystallographic characterization by Schlapschy, Martin, Dommel, Monica K, Hadian, Kamyar, Fogarasi, Marton, Korndörfer, Ingo P, Skerra, Arne

“…The 'seventeen kilodalton protein' Skp confers transient solubility on outer membrane proteins during biogenesis in Gram-negative bacteria. Here we report a…”
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Efficient secretory production of proline/alanine/serine by Friedrich, L, Kikuchi, Y, Matsuda, Y, Binder, U, Skerra, A

“…PAS biopolymers are recombinant polypeptides comprising the small uncharged l-amino acids Pro, Ala and/or Ser which resemble the widely used poly-ethylene…”
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Bifunctional and multimeric complexes of streptavidin fused to single chain antibodies (scFv) by Dübel, S., Breitling, F., Kontermann, R., Schmidt, T., Skerra, A., Little, M.

“…Multivalent and multispecific antibodies with defined stoichiometry could provide valuable tools for biological and medical research and for the diagnosis and…”
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