Colony establishment and maintenance of the eriophyid wheat curl mite Aceria tosichella for controlled transmission studies on a new virus-like pathogen

High plains disease (HPD) is of serious economic concern for wheat and corn production, but little is known about the virus-like causal agent. In the field, HPD is often associated with Wheat streak mosaic virus (WSMV) and both pathogens are transmitted by the same eriophyid wheat curl mite, Aceria...

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Bibliographic Details
Published in:	Journal of virological methods Vol. 108; no. 1; pp. 133 - 137
Main Authors:	Skare, J.M., Wijkamp, I., Rezende, J., Michels, G., Rush, C., Scholthof, K.-B.G., Scholthof, H.B.
Format:	Journal Article
Language:	English
Published:	London Elsevier B.V 01-03-2003 Amsterdam Elsevier New York, NY
Subjects:	Animals Arachnid Vectors - pathogenicity Arachnid Vectors - virology Biological and medical sciences Colony establishment and maintenance Eriophyid mites Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control HPD Immuno-detection Microbiology Mites - pathogenicity Mites - virology Mosaic Viruses - pathogenicity Phytopathology. Animal pests. Plant and forest protection Plant Diseases - parasitology Plant Diseases - virology Plant Viruses - pathogenicity Plant viruses and viroids Techniques used in virology Transmission Triticum - parasitology Triticum - virology Virology Virology - methods Eriophyid mites Immuno-detection Colony establishment and maintenance HPD Transmission Monocotyledones Plant pathogen Cereal crop Immunological method Acari Gramineae Angiospermae Presumed viral disease Vector Infection Acariformes Arachnida Eriophyidae Actinedida Arthropoda Colony Spermatophyta Invertebrata Detection Triticum aestivum
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Summary:	High plains disease (HPD) is of serious economic concern for wheat and corn production, but little is known about the virus-like causal agent. In the field, HPD is often associated with Wheat streak mosaic virus (WSMV) and both pathogens are transmitted by the same eriophyid wheat curl mite, Aceria tosichella Keifer. The objective of this study was to develop methods for establishing and maintaining HPD-transmitting wheat curl mite colonies for their use in studies on HPD. Towards this goal, mite colonies from a mixed infection source were separated into colonies either (i) not viruliferous; (ii) only transmitting WSMV; or (iii) only transmitting HPD. Maintenance of these colonies required strictly separated incubator facilities and adaptation of mite-suitable transfer techniques to permit frequent passages of mites to healthy plants. The established colonies provided reliable sources of infective material to study the progression of HPD and/or WSMV in plants using sensitive immuno-detection assays. In conclusion, we have developed reliable methods with a poorly studied arthropod vector to examine the biology and properties of a new virus-like disease.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0166-0934 1879-0984
DOI:	10.1016/S0166-0934(02)00257-4