Search Results - "Siwkowski, A."

Reducing glycosphingolipid biosynthesis in airway cells partially ameliorates disease manifestations in a mouse model of asthma by Karman, Jozsef, Tedstone, Jennifer L., Gumlaw, Nathan K., Zhu, Yunxiang, Yew, Nelson, Siegel, Craig, Guo, Shuling, Siwkowski, Andrew, Ruzek, Melanie, Jiang, Canwen, Cheng, Seng H.

“…Lipid rafts reportedly play an important role in modulating the activation of mast cells and granulocytes, the primary effector cells of airway…”
Get full text

Design, Synthesis And Evaluation Of Constrained Methoxyethyl (cMOE) and Constrained Ethyl (cEt) Nucleoside Analogs by Seth, Punit P., Siwkowski, Andrew, Allerson, Charles R., Vasquez, Guillermo, Lee, Sam, Prakash, Thazha P., Kinberger, Garth, Migawa, Michael T., Gaus, Hans, Bhat, Balkrishen, Swayze, Eric E.

“…Abstract Antisense drug discovery technology is a powerful method to modulate gene expression in animals and represents a novel therapeutic platform.1 We have…”
Get full text

Antisense oligonucleotide therapy for TTR amyloidosis by Benson, M. D., Pandey, S., Witchell, D., Jazayeri, A., Siwkowski, A., Monia, B., Kluve-Beckerman, B.

Get full text

T7 transcript length determination using enzymatic RNA sequencing by Siwkowski, A

Get more information

Identification and functional validation of PNAs that inhibit murine CD40 expression by redirection of splicing by Siwkowski, Andrew M., Malik, Leila, Esau, Christine C., Maier, Martin A., Wancewicz, Edward V., Albertshofer, Klaus, Monia, Brett P., Bennett, C. Frank, Eldrup, Anne B.

“…Cognate recognition between the CD40 receptor and its ligand, CD154, is thought to play a central role in the initiation and propagation of immune responses…”
Get full text

Structure−Activity Relationship Study on a Simple Cationic Peptide Motif for Cellular Delivery of Antisense Peptide Nucleic Acid by Albertshofer, Klaus, Siwkowski, Andrew M, Wancewicz, Edward V, Esau, Christine C, Watanabe, Tanya, Nishihara, Kenji C, Kinberger, Garth A, Malik, Leila, Eldrup, Anne B, Manoharan, Muthiah, Geary, Richard S, Monia, Brett P, Swayze, Eric E, Griffey, Richard H, Bennett, C. Frank, Maier, Martin A

“…Improving cellular uptake and biodistribution remains one of the major obstacles for a successful and broad application of peptide nucleic acids (PNAs) as…”
Get full text

Evaluation of Basic Amphipathic Peptides for Cellular Delivery of Antisense Peptide Nucleic Acids by Maier, Martin A., Esau, Christine C., Siwkowski, Andrew M., Wancewicz, Edward V., Albertshofer, Klaus, Kinberger, Garth A., Kadaba, Neena S., Watanabe, Tanya, Manoharan, Muthiah, Bennett, C. Frank, Griffey, Richard H., Swayze, Eric E.

“…Cellular permeation peptides have been used successfully for the delivery of a variety of cargoes across cellular membranes, including large hydrophilic…”
Get full text

Oligonucleotide-europium complex conjugate designed to cleave the 5′ cap structure of the ICAM-1 transcript potentiates antisense activity in cells by Baker, Brenda F., Lot, Sidney S., Kringel, Jim, Cheng-Flournoy, Shin, Villiet, Pierre, Sasmor, Henri M., Siwkowski, Andrew M., Chappell, Lara L., Morrow, Janet R.

“…The 5′ cap structure of mRNA is a N7 methylated guanosine residue that is linked by a 5′-5′ triphosphate linkage to the 5′-terminus of cellular and viral RNAs…”
Get full text

Determination of catalytic parameters for hairpin ribozymes by DeYoung, M B, Siwkowski, A, Hampel, A

Get more information

The sCYMV1 hairpin ribozyme : targeting rules and cleavage of heterologous RNA by LIAN, Y, DE YOUNG, M. B, SIWKOWSKI, A, HAMPEL, A, RAPPAPORT, J

“…The catalytic center of the RNA from the negative strand of the satellite RNA of chicory yellow mottle virus type 1 (sCYMV1) is in the hairpin ribozyme family,…”
Get full text

Design of the hairpin ribozyme for targeting specific RNA sequences by Hampel, A, DeYoung, M B, Galasinski, S, Siwkowski, A

“…The following steps should be taken when designing the hairpin ribozyme to cleave a specific target sequence: 1. Select a target sequence containing BN*GUC…”
Get more information

Mutagenesis and modeling of the hairpin ribozyme family by Siwkowski, A, DeYoung, M B, Anderson, P, Hampel, A

Get more information

Discovery and Analysis of Antisense Oligonucleotide Activity in Cell Culture by Baker, Brenda F., Condon, Thomas P., Koller, Erich, McKay, Robert A., Siwkowski, Andrew M., Vickers, Timothy A., Monia, Brett P.

“…In the past decade antisense oligonucleotides (ASOs) have proven to be a useful tool for dissection of gene function in molecular cell biology (Koller, E.,…”
Get full text

Catalytic Properties of Hairpin Ribozymes Derived from Chicory Yellow Mottle Virus and Arabis Mosaic Virus Satellite RNAs by DeYoung, Mary Beth, Siwkowski, Andrew M, Lian, Ying, Hampel, Arnold

“…Regions of the negative strands of the satellite RNAs of chicory yellow mottle virus (sCYMV1) and arabis mosaic virus (sArMV) have similarity in sequence and…”
Get full text

Antisense oligonucleotide blockade of tumor necrosis factor-alpha in two murine models of colitis by Myers, Kathleen J, Murthy, Sreekant, Flanigan, Anne, Witchell, Donna R, Butler, Madeline, Murray, Susan, Siwkowski, Andrew, Goodfellow, Deborah, Madsen, Karen, Baker, Brenda

“…Tumor necrosis factor-alpha (TNF-alpha) is a key cytokine involved in the pathogenesis of inflammatory bowel disease. We have developed a second-generation…”
Get more information

Mutational Analysis of Loops 1 and 5 of the Hairpin Ribozyme by Shippy, Richard, Siwkowski, Andrew, Hampel, Arnold

“…A comprehensive analysis of base preferences for all positions in loops 1 and 5 of the hairpin ribozyme−substrate complex was carried out using a cis-ribozyme…”
Get full text

Analysis of Hairpin Ribozyme Base Mutations in Loops 2 and 4 and Their Effects on cis-Cleavage in Vitro by Siwkowski, Andrew, Shippy, Richard, Hampel, Arnold

“…In order to determine base requirements in loops 2 and 4 of the hairpin ribozyme, a comprehensive mutational analysis of the wild type sequence was done. Each…”
Get full text

Inhibition of HPV-16 E6/E7 Immortalization of Normal Keratinocytes by Hairpin Ribozymes by Alvarez-Salas, Luis M., Cullinan, Amy E., Siwkowski, Andrew, Hampel, Arnold, DiPaolo, Joseph A.

“…HPV-16 E6 and E7 genes are required to efficiently immortalize a broad spectrum of cell types including cervical keratinocytes. Therefore, the E6/E7 genes can…”
Get full text

Alteration of hairpin ribozyme specificity utilizing PCR by DeGrandis, P, Hampel, A, Galasinski, S, Borneman, J, Siwkowski, A, Altschuler, M

“…We have developed a method by which a researcher can quickly alter the specificity of a trans hairpin ribozyme. Utilizing this PCR method, two…”
Get more information

Screening for important base identities in the hairpin ribozyme by in vitro selection for cleavage by Siwkowski, A, Humphrey, M, De-Young, M B, Hampel, A

“…Random mutagenesis followed by an in vitro selection procedure was shown to be capable of identifying important bases of the hairpin ribozyme for cleavage of…”
Get full text